Detailed information of TA system TA030994

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /SpoIISA(toxin)
• Location: 3307457..3308435
• Replicon: chromosome
• Accession: NZ_AP014864
• Organism: Bacillus thuringiensis serovar tolworthi strain Pasteur Institute Standard strain

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: W8Y388
• Locus tag: KNN_RS16915
• Protein ID: WP_000624977.1
• Coordinates: 3307698..3308435 (-)
• Length: 246 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: M1PKC2
• Locus tag: KNN_RS16910
• Protein ID: WP_000237819.1
• Coordinates: 3307457..3307585 (-)
• Length: 43 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KNN_RS16885	3303439..3304041	-	603	WP_000517271.1	hypothetical protein	-
KNN_RS16890	3304067..3304876	-	810	WP_000678369.1	C39 family peptidase	-
KNN_RS16895	3305122..3306591	-	1470	WP_048656315.1	beta-Ala-His dipeptidase	-
KNN_RS16900	3306803..3307192	+	390	WP_000713869.1	YxeA family protein	-
KNN_RS16905	3307208..3307384	-	177	WP_000852629.1	stage II sporulation protein SB	-
KNN_RS16910	3307457..3307585	-	129	WP_000237819.1	hypothetical protein	Antitoxin
KNN_RS16915	3307698..3308435	-	738	WP_000624977.1	stage II sporulation protein SA	Toxin
KNN_RS16920	3308674..3309411	-	738	WP_000594152.1	2,3-diphosphoglycerate-dependent phosphoglycerate mutase	-
KNN_RS16925	3309578..3310060	-	483	WP_000191888.1	MarR family winged helix-turn-helix transcriptional regulator	-
KNN_RS16930	3310168..3311854	+	1687	Protein_3402	alpha-keto acid decarboxylase family protein	-
KNN_RS16935	3312012..3312794	-	783	WP_000355674.1	class I SAM-dependent methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (10-243)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 246 a.a.
• Molecular weight: 28307.03 Da
• Isoelectric Point: 8.2998

>T30994 WP_000624977.1 NZ_AP014864:c3308435-3307698 [Bacillus thuringiensis serovar tolworthi]
MISNIRIGLFVLAIVFVVLVFFYWKNEELYEEKKQRIRKTWYGLFIISVTVYFMIKGIDLTLWKNLLMFTAMVIFVDIAF
ILTPNISEIWGAKFSDIGKTVQSIKRSLIASKARGEIYTTIIQNVNPAVFGTMEWHTEEEYTKSLNAFLDSYGEKIGAKI
VVFEAAKELNTNFRGIRSQFSIIVPLEHIEQLNEQKAVQVENVGIIPAKIVSDVFIVIDGKKNNLQDRDFENVYNLTIHH
SYFSK

Nucleotide

• Length: 738 bp

>T30994 NZ_AP014864:c3308435-3307698 [Bacillus thuringiensis serovar tolworthi]
ATGATCTCTAACATTCGAATTGGCTTATTTGTTTTAGCGATTGTCTTTGTAGTCCTTGTTTTCTTTTATTGGAAAAATGA
GGAGTTGTACGAGGAGAAAAAGCAACGTATTAGAAAGACTTGGTATGGTTTGTTTATCATATCTGTCACCGTGTATTTCA
TGATAAAAGGAATTGATTTAACCCTCTGGAAAAATCTTTTAATGTTTACTGCAATGGTTATTTTTGTTGATATTGCGTTC
ATTTTAACGCCTAATATTTCAGAAATATGGGGTGCGAAATTTAGCGATATTGGCAAGACAGTTCAATCAATAAAACGATC
ATTAATTGCATCTAAAGCGAGAGGGGAAATATACACGACAATTATTCAAAATGTTAATCCAGCAGTATTCGGTACGATGG
AATGGCATACGGAAGAGGAATATACAAAGAGCTTAAATGCATTTTTAGATTCATATGGGGAAAAGATTGGCGCGAAAATT
GTTGTTTTTGAAGCCGCGAAAGAGTTAAATACAAACTTTCGTGGTATTCGCTCGCAATTTAGTATTATCGTTCCGTTAGA
ACATATCGAGCAATTGAATGAGCAGAAAGCGGTGCAAGTAGAAAATGTCGGGATTATACCAGCAAAAATAGTAAGTGACG
TTTTCATTGTTATTGATGGAAAGAAAAATAACCTGCAAGATCGGGATTTTGAAAATGTATATAATTTAACAATACATCAT
AGTTATTTTAGTAAATAA

Antitoxin

Protein

• Length: 43 a.a.
• Molecular weight: 4953.36 Da
• Isoelectric Point: 3.9798

>AT30994 WP_000237819.1 NZ_AP014864:c3307585-3307457 [Bacillus thuringiensis serovar tolworthi]
VVKDYDNDFFKEGNEDTTDFSLIKGDTLQQVEELEEELKRKS

Nucleotide

• Length: 129 bp

>AT30994 NZ_AP014864:c3307585-3307457 [Bacillus thuringiensis serovar tolworthi]
GTGGTAAAAGATTATGACAATGATTTTTTTAAAGAAGGTAACGAAGATACAACAGATTTCTCTTTAATAAAAGGGGATAC
GTTGCAGCAAGTAGAAGAATTAGAAGAAGAATTAAAAAGAAAATCATAA

Structures

Toxin

• AlphaFold DB: W8Y388

Antitoxin

• AlphaFold DB: M1PKC2