Detailed information of TA system TA030993

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/upstrm_HI1419-dnstrm_HI1420
• Location: 44578..45172
• Replicon: plasmid pKF707
• Accession: NZ_AP014863
• Organism: Pseudomonas furukawaii strain KF707

Toxin (Protein)

• Gene name: relE
• Uniprot ID: L8MJ46
• Locus tag: KF707C_RS28845
• Protein ID: WP_003454279.1
• Coordinates: 44578..44877 (+)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: L8MM80
• Locus tag: KF707C_RS28850
• Protein ID: WP_003454278.1
• Coordinates: 44882..45172 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KF707C_RS28815	40206..40667	-	462	WP_036993412.1	hypothetical protein	-
KF707C_RS28825	41093..41407	-	315	WP_036993410.1	hypothetical protein	-
KF707C_RS29405	41483..41623	-	141	WP_158526761.1	hypothetical protein	-
KF707C_RS28830	41711..42130	-	420	WP_003454280.1	antirestriction protein	-
KF707C_RS28835	43093..43929	-	837	WP_131679688.1	hypothetical protein	-
KF707C_RS28840	44117..44362	-	246	WP_051050753.1	hypothetical protein	-
KF707C_RS28845	44578..44877	+	300	WP_003454279.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KF707C_RS28850	44882..45172	+	291	WP_003454278.1	putative addiction module antidote protein	Antitoxin
KF707C_RS28855	45216..45506	+	291	WP_036993407.1	hypothetical protein	-
KF707C_RS28860	45532..45747	+	216	WP_036993406.1	hypothetical protein	-
KF707C_RS28865	46199..46441	-	243	WP_036990823.1	hypothetical protein	-
KF707C_RS28870	46529..47686	-	1158	WP_003448181.1	ParB/RepB/Spo0J family partition protein	-
KF707C_RS28875	47686..48450	-	765	WP_036990821.1	ParA family protein	-
KF707C_RS28880	48447..48749	-	303	WP_036990819.1	hypothetical protein	-
KF707C_RS28885	48844..49197	-	354	WP_036990817.1	hypothetical protein	-
KF707C_RS28890	49400..50161	-	762	WP_036990825.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..59815	59815

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (5-95)

Antitoxin

• Uncharacterized protein HI1420 (2-93)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11000.67 Da
• Isoelectric Point: 8.8913

>T30993 WP_003454279.1 NZ_AP014863:44578-44877 [Pseudomonas furukawaii]
MNSFISTDAFDNWLASLTDKVGKARILQRLDSAMLGNFGDCEPVGEGVSEMRIHYGPGYRVYFTRQGTVVYLLLLGGDKS
SQKRDIKLAKKLLAELGKE

Nucleotide

• Length: 300 bp

>T30993 NZ_AP014863:44578-44877 [Pseudomonas furukawaii]
ATGAACTCCTTCATTAGCACCGACGCTTTCGACAACTGGCTGGCCAGCCTGACCGACAAGGTTGGAAAGGCCCGCATCCT
GCAGCGACTCGACTCGGCAATGCTCGGCAACTTCGGTGACTGCGAGCCGGTGGGGGAAGGCGTCAGCGAGATGCGAATCC
ATTACGGCCCCGGCTACCGGGTGTACTTCACCCGCCAGGGCACAGTCGTTTATCTGCTTCTGCTCGGCGGCGATAAGTCG
TCCCAGAAGCGCGACATTAAACTGGCCAAGAAACTGCTCGCAGAACTTGGCAAGGAGTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10155.70 Da
• Isoelectric Point: 6.2089

>AT30993 WP_003454278.1 NZ_AP014863:44882-45172 [Pseudomonas furukawaii]
MTTLTPFDVSKHLDDDEVIGAYLSAALEDPNPDAFLLALHNVAKAKGMSQIAKDAGVGRESLYKALAPGAKPRYDTILKL
LHALGLQLVVSPSQAA

Nucleotide

• Length: 291 bp

>AT30993 NZ_AP014863:44882-45172 [Pseudomonas furukawaii]
ATGACCACCCTAACCCCCTTCGATGTATCCAAACACCTCGACGATGATGAGGTGATCGGTGCCTATTTGAGTGCTGCCCT
GGAGGATCCGAATCCCGACGCCTTCCTCCTTGCTCTGCACAACGTGGCGAAGGCTAAAGGCATGTCACAGATCGCGAAGG
ACGCTGGCGTAGGACGCGAAAGTCTCTACAAGGCACTGGCCCCAGGTGCAAAGCCGAGATACGACACCATCCTGAAGCTG
CTGCATGCGCTTGGCCTTCAGTTGGTCGTCAGTCCGAGTCAGGCCGCCTAA

Structures

Toxin

• AlphaFold DB: L8MJ46

Antitoxin

• AlphaFold DB: L8MM80