Detailed information of TA system TA030991

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 5430762..5431387
• Replicon: chromosome
• Accession: NZ_AP014862
• Organism: Pseudomonas furukawaii strain KF707

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: L8MJ87
• Locus tag: KF707C_RS25225
• Protein ID: WP_003452675.1
• Coordinates: 5431205..5431387 (-)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: KF707C_RS25220
• Protein ID: WP_036993140.1
• Coordinates: 5430762..5431175 (-)
• Length: 138 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KF707C_RS25195	5426145..5426612	+	468	WP_003452668.1	LEA type 2 family protein	-
KF707C_RS25200	5426796..5427137	+	342	WP_036993112.1	alkylphosphonate utilization protein	-
KF707C_RS25205	5427334..5428719	+	1386	WP_036993114.1	sodium:solute symporter	-
KF707C_RS25210	5428769..5429722	+	954	WP_003452672.1	agmatinase	-
KF707C_RS25215	5429799..5430716	+	918	WP_003452673.1	LysR family transcriptional regulator	-
KF707C_RS25220	5430762..5431175	-	414	WP_036993140.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
KF707C_RS25225	5431205..5431387	-	183	WP_003452675.1	type II toxin-antitoxin system HicA family toxin	Toxin
KF707C_RS25230	5431521..5432309	+	789	WP_003452676.1	NYN domain-containing protein	-
KF707C_RS25235	5432708..5433241	+	534	WP_003452677.1	transposase	-
KF707C_RS25240	5433306..5434802	-	1497	WP_003452678.1	CoA-acylating methylmalonate-semialdehyde dehydrogenase	-
KF707C_RS25245	5434907..5435194	-	288	WP_003452680.1	cupin domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-128)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6753.95 Da
• Isoelectric Point: 11.3688

>T30991 WP_003452675.1 NZ_AP014862:c5431387-5431205 [Pseudomonas furukawaii]
VNSRQLVELIEADGWFLVRVRGSHHHFKHPSKPGLVTIPHPKKDLLPRTVASILKQAGLK

Nucleotide

• Length: 183 bp

>T30991 NZ_AP014862:c5431387-5431205 [Pseudomonas furukawaii]
GTGAACAGTCGACAACTCGTGGAGCTGATCGAGGCCGATGGATGGTTTCTGGTCAGGGTCAGGGGCAGTCACCACCACTT
CAAGCACCCGAGCAAACCGGGGTTGGTGACTATTCCCCATCCGAAGAAAGACCTGCTGCCCAGAACGGTGGCCAGCATTC
TCAAACAGGCGGGGCTCAAATGA

Antitoxin

Protein

• Length: 138 a.a.
• Molecular weight: 14779.84 Da
• Isoelectric Point: 4.6295

>AT30991 WP_036993140.1 NZ_AP014862:c5431175-5430762 [Pseudomonas furukawaii]
MLFPIAILPGDDGHAWGVEVPDLPGCISAGDDLDDAMAMAHEAIEGHLELLAEEGAPIPVARKLTFHAANPDYTGCTWAL
VDIDVTRYMGKAEKLNITLPGHLLTRIDAYVRSHPEQKSRSGFLAAAALKVLQEEHS

Nucleotide

• Length: 414 bp

>AT30991 NZ_AP014862:c5431175-5430762 [Pseudomonas furukawaii]
ATGCTGTTCCCGATCGCAATACTGCCTGGCGACGATGGGCACGCCTGGGGCGTGGAGGTGCCGGACCTGCCGGGCTGCAT
CTCGGCGGGCGATGACCTGGACGATGCGATGGCCATGGCCCACGAGGCCATCGAGGGGCACCTGGAGTTGCTGGCGGAGG
AGGGGGCGCCGATTCCGGTGGCGAGGAAGCTGACCTTCCATGCGGCGAACCCGGACTACACAGGATGCACCTGGGCGCTG
GTGGATATCGACGTGACGCGCTACATGGGCAAGGCGGAGAAGCTGAACATCACCCTGCCGGGCCACCTGCTCACTCGCAT
CGATGCGTATGTCCGCAGCCATCCGGAGCAGAAGAGCCGCTCGGGGTTCCTGGCTGCGGCGGCGTTGAAGGTGTTGCAGG
AGGAGCATTCGTAG

Structures

Toxin

• AlphaFold DB: L8MJ87