Detailed information of TA system TA030990

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpA-stbD/BrnT_toxin-DnaT
• Location: 5235801..5236475
• Replicon: chromosome
• Accession: NZ_AP014862
• Organism: Pseudomonas furukawaii strain KF707

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: L8MTH5
• Locus tag: KF707C_RS24345
• Protein ID: WP_004422797.1
• Coordinates: 5235801..5236079 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: stbD
• Uniprot ID: -
• Locus tag: KF707C_RS24350
• Protein ID: WP_004422799.1
• Coordinates: 5236200..5236475 (-)
• Length: 92 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KF707C_RS29215	5232042..5232461	-	420	WP_131679701.1	hypothetical protein	-
KF707C_RS24320	5232464..5233759	-	1296	WP_069776297.1	ATP-binding protein	-
KF707C_RS24325	5233855..5234154	-	300	WP_036994308.1	hypothetical protein	-
KF707C_RS24330	5234151..5235020	-	870	WP_004422790.1	restriction endonuclease	-
KF707C_RS24335	5235171..5235515	+	345	WP_036994313.1	hypothetical protein	-
KF707C_RS24340	5235560..5235823	-	264	WP_004422795.1	BrnA antitoxin family protein	-
KF707C_RS24345	5235801..5236079	-	279	WP_004422797.1	BrnT family toxin	Toxin
KF707C_RS24350	5236200..5236475	-	276	WP_004422799.1	hypothetical protein	Antitoxin
KF707C_RS24355	5236790..5237050	-	261	WP_004422800.1	Txe/YoeB family addiction module toxin	-
KF707C_RS24360	5237050..5237322	-	273	WP_004422802.1	type II toxin-antitoxin system prevent-host-death family antitoxin	-
KF707C_RS24365	5237506..5237925	-	420	WP_036994315.1	type II toxin-antitoxin system VapC family toxin	-
KF707C_RS24370	5237922..5238167	-	246	WP_036994317.1	Arc family DNA-binding protein	-
KF707C_RS24375	5238203..5238376	-	174	Protein_4791	integrase	-
KF707C_RS29380	5238403..5238576	-	174	WP_004422807.1	hypothetical protein	-
KF707C_RS24380	5239027..5239245	+	219	WP_036994319.1	hypothetical protein	-
KF707C_RS24385	5239306..5240301	-	996	WP_004422810.1	isocitrate/isopropylmalate dehydrogenase family protein	-
KF707C_RS24390	5240306..5241220	-	915	WP_036994327.1	DMT family transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	5218249..5238382	20133

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-82)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10977.40 Da
• Isoelectric Point: 7.2038

>T30990 WP_004422797.1 NZ_AP014862:c5236079-5235801 [Pseudomonas furukawaii]
MRFEWDEDKNQANIRKHGIDFNDVPDMFQHPRVVLRDDRTDYGEERWISLGWIKALVGVVVYTERRGDVIRIISARKATK
QEAKRYVESIEN

Nucleotide

• Length: 279 bp

>T30990 NZ_AP014862:c5236079-5235801 [Pseudomonas furukawaii]
ATGCGCTTTGAATGGGACGAAGACAAGAACCAGGCCAACATCCGCAAGCACGGGATCGACTTCAATGACGTGCCGGATAT
GTTTCAGCACCCACGGGTGGTTTTGCGCGATGACCGTACCGATTACGGCGAAGAGCGCTGGATCAGCCTGGGGTGGATCA
AGGCGCTGGTAGGCGTGGTCGTATACACCGAGCGGCGGGGTGACGTGATCCGCATTATCTCTGCCCGAAAAGCCACCAAG
CAGGAGGCCAAACGCTATGTCGAAAGCATCGAAAACTGA

Antitoxin

Protein

• Length: 92 a.a.
• Molecular weight: 9931.29 Da
• Isoelectric Point: 4.0490

>AT30990 WP_004422799.1 NZ_AP014862:c5236475-5236200 [Pseudomonas furukawaii]
MAFPVLADLAISLTDFKRDPIGAVRKGQGETVLVLDDDQPAFYAVPPAQYLALLERIDDLQLSELVRHRQGEPVVAVDID
DLIKEAAETSG

Nucleotide

• Length: 276 bp

>AT30990 NZ_AP014862:c5236475-5236200 [Pseudomonas furukawaii]
ATGGCCTTTCCGGTTCTGGCTGATCTTGCCATCTCCCTCACCGACTTCAAGCGTGACCCAATCGGCGCCGTCCGCAAAGG
GCAGGGTGAAACGGTCCTGGTCCTCGATGACGATCAACCGGCCTTCTATGCCGTTCCGCCTGCGCAGTACCTGGCCCTGT
TGGAACGGATCGATGACCTGCAACTGTCCGAACTGGTCCGCCATCGCCAGGGGGAGCCGGTCGTGGCTGTGGATATCGAT
GATCTGATCAAGGAAGCAGCCGAAACCAGCGGCTGA

Structures

Toxin

• AlphaFold DB: L8MTH5