Detailed information of TA system TA030988

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 4664445..4665086
• Replicon: chromosome
• Accession: NZ_AP014862
• Organism: Pseudomonas furukawaii strain KF707

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: L8MIE7
• Locus tag: KF707C_RS21490
• Protein ID: WP_003450374.1
• Coordinates: 4664679..4665086 (+)
• Length: 136 a.a.

Antitoxin (Protein)

• Gene name: VapB1
• Uniprot ID: L8MG90
• Locus tag: KF707C_RS21485
• Protein ID: WP_003450375.1
• Coordinates: 4664445..4664678 (+)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KF707C_RS21465	4659834..4661840	+	2007	WP_003450379.1	methyl-accepting chemotaxis protein	-
KF707C_RS21470	4661870..4662715	+	846	WP_003450378.1	chemotaxis protein CheR	-
KF707C_RS21475	4662712..4663323	+	612	WP_003450377.1	hypothetical protein	-
KF707C_RS21480	4663328..4664377	+	1050	WP_003450376.1	chemotaxis response regulator protein-glutamate methylesterase	-
KF707C_RS21485	4664445..4664678	+	234	WP_003450375.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
KF707C_RS21490	4664679..4665086	+	408	WP_003450374.1	type II toxin-antitoxin system VapC family toxin	Toxin
KF707C_RS21495	4665091..4665477	-	387	WP_003450373.1	DUF1850 domain-containing protein	-
KF707C_RS21500	4665474..4667501	-	2028	WP_003450372.1	TRAP transporter permease	-
KF707C_RS21505	4667581..4668537	-	957	WP_003450371.1	TAXI family TRAP transporter solute-binding subunit	-
KF707C_RS21510	4668830..4669867	+	1038	WP_003450370.1	metal-dependent hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-42)

Sequences

Toxin

Protein

• Length: 136 a.a.
• Molecular weight: 15054.17 Da
• Isoelectric Point: 6.2345

>T30988 WP_003450374.1 NZ_AP014862:4664679-4665086 [Pseudomonas furukawaii]
MSVRYLLDTNICIYIAKHNPARVRERFAAHASAELGMSVITLGELRFGAEKSQSRERALAVIERLESLIQVLPLEETAGA
HYGQIRAELSARGQIIGGNDLWIAAQAQAADLTLVSNNLREFQRIPDLRLENWAD

Nucleotide

• Length: 408 bp

>T30988 NZ_AP014862:4664679-4665086 [Pseudomonas furukawaii]
ATGTCGGTGCGCTACCTGCTGGACACCAACATCTGCATCTACATCGCCAAGCACAATCCCGCCAGGGTGCGCGAACGCTT
TGCGGCGCATGCCTCGGCCGAGCTCGGCATGTCGGTCATCACCTTGGGCGAGCTTCGCTTCGGGGCTGAAAAGAGTCAGT
CGAGGGAACGCGCCCTGGCGGTGATCGAGCGACTGGAAAGCCTGATCCAGGTACTCCCGCTGGAGGAAACCGCCGGAGCG
CATTACGGCCAGATCAGGGCGGAGTTGTCGGCCCGTGGACAGATCATCGGCGGTAACGACCTCTGGATCGCCGCCCAGGC
CCAGGCGGCGGATCTGACACTGGTGAGCAACAACCTCCGGGAGTTCCAGCGAATTCCGGACTTGCGGCTGGAAAACTGGG
CGGACTGA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8993.12 Da
• Isoelectric Point: 4.3654

>AT30988 WP_003450375.1 NZ_AP014862:4664445-4664678 [Pseudomonas furukawaii]
MPFAKLFQSGNSQAVRLPKEFRFKNVDRVEIFRRGNEIVLREISTNATDIFDALVEMPDDFMEGGRDDLPPQEREDF

Nucleotide

• Length: 234 bp

>AT30988 NZ_AP014862:4664445-4664678 [Pseudomonas furukawaii]
ATGCCCTTCGCCAAGCTCTTTCAATCCGGCAACAGCCAGGCCGTCAGACTGCCCAAGGAATTCCGCTTCAAGAATGTGGA
TCGCGTCGAAATCTTCCGTCGCGGCAACGAGATCGTGCTGCGTGAGATATCGACCAACGCGACGGACATCTTCGACGCAC
TGGTGGAAATGCCGGATGACTTCATGGAAGGCGGTCGGGACGACCTGCCTCCCCAGGAACGGGAGGACTTCTGA

Structures

Toxin

• AlphaFold DB: L8MIE7

Antitoxin

• AlphaFold DB: L8MG90