Detailed information of TA system TA030986

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 3635753..3636433
• Replicon: chromosome
• Accession: NZ_AP014862
• Organism: Pseudomonas furukawaii strain KF707

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: L8MKH0
• Locus tag: KF707C_RS16920
• Protein ID: WP_003456079.1
• Coordinates: 3635753..3636178 (-)
• Length: 142 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: L8MQV0
• Locus tag: KF707C_RS16925
• Protein ID: WP_003456080.1
• Coordinates: 3636185..3636433 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KF707C_RS16895	3631077..3631418	-	342	WP_003456068.1	ribonuclease E inhibitor RraB	-
KF707C_RS16900	3631688..3632632	-	945	WP_003456072.1	alpha/beta hydrolase	-
KF707C_RS16905	3632616..3634082	-	1467	WP_003456074.1	NAD(P)/FAD-dependent oxidoreductase	-
KF707C_RS16910	3634242..3634520	-	279	WP_003456076.1	hypothetical protein	-
KF707C_RS16915	3634706..3635731	+	1026	WP_003456078.1	patatin-like phospholipase family protein	-
KF707C_RS16920	3635753..3636178	-	426	WP_003456079.1	type II toxin-antitoxin system VapC family toxin	Toxin
KF707C_RS16925	3636185..3636433	-	249	WP_003456080.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
KF707C_RS16930	3636603..3637532	-	930	WP_003456081.1	malate dehydrogenase	-
KF707C_RS16935	3637789..3638181	+	393	WP_036993955.1	hypothetical protein	-
KF707C_RS16940	3638285..3638755	+	471	WP_003456085.1	hypothetical protein	-
KF707C_RS16945	3638830..3639078	+	249	WP_036993957.1	DUF2933 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-131)

Antitoxin

• SpoVT-AbrB domain (9-46)

Sequences

Toxin

Protein

• Length: 142 a.a.
• Molecular weight: 15086.46 Da
• Isoelectric Point: 5.8075

>T30986 WP_003456079.1 NZ_AP014862:c3636178-3635753 [Pseudomonas furukawaii]
MPTVVTYMLDTNICSFIIRKNPPQVLERLQCAAAAGNRLVISAITYAELRYGAASPRAPKALTAWIDALVQRLDEILPWN
DTAVEASANLMAQLLKAGTPIGPNDTGIAGHALSTGCIVVTNNTREFLRVPGLLVEDWAAG

Nucleotide

• Length: 426 bp

>T30986 NZ_AP014862:c3636178-3635753 [Pseudomonas furukawaii]
ATGCCGACTGTCGTGACCTATATGCTGGATACGAATATCTGCTCGTTCATCATCCGGAAGAACCCGCCGCAGGTGTTGGA
GCGTCTGCAGTGCGCTGCCGCTGCCGGCAATCGGTTGGTGATTTCGGCCATCACCTACGCCGAGCTTCGCTACGGCGCCG
CCTCGCCTCGCGCGCCCAAGGCGTTGACGGCCTGGATCGATGCCCTGGTGCAGCGCCTGGACGAAATTCTGCCCTGGAAC
GATACGGCCGTTGAGGCCTCTGCGAACTTGATGGCCCAGTTGCTGAAGGCAGGCACGCCGATCGGCCCGAACGACACCGG
AATTGCAGGGCACGCACTCTCCACCGGCTGCATTGTTGTCACCAACAACACACGGGAGTTTCTGCGTGTGCCTGGTCTTC
TCGTAGAGGATTGGGCCGCTGGCTGA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9286.48 Da
• Isoelectric Point: 4.5994

>AT30986 WP_003456080.1 NZ_AP014862:c3636433-3636185 [Pseudomonas furukawaii]
MATITVSLFRNGRNQAIRIPRDMEFHGVTELIVTREGDTLTLRPSRPSWSSLATMEPIEADFLADRPAVIEPGRVDFSER
ED

Nucleotide

• Length: 249 bp

>AT30986 NZ_AP014862:c3636433-3636185 [Pseudomonas furukawaii]
ATGGCCACTATCACTGTTTCGTTGTTCCGCAATGGTCGGAATCAGGCCATTCGAATCCCCCGGGACATGGAGTTCCACGG
CGTGACCGAGTTGATCGTGACCCGTGAAGGCGACACGCTGACGCTGCGGCCGTCGCGCCCCTCCTGGAGCAGCCTGGCGA
CGATGGAGCCCATCGAGGCGGACTTCCTGGCGGATCGTCCTGCGGTCATCGAGCCAGGCCGGGTCGACTTCTCTGAACGC
GAGGATTGA

Structures

Toxin

• AlphaFold DB: L8MKH0

Antitoxin

• AlphaFold DB: L8MQV0