Detailed information of TA system TA030973

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataRT/DUF1778(antitoxin)
• Location: 3559797..3560597
• Replicon: chromosome
• Accession: NZ_AP014857
• Organism: Escherichia albertii strain EC06-170

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: A0A8H9C5V7
• Locus tag: AST80_RS17435
• Protein ID: WP_000342455.1
• Coordinates: 3559797..3560324 (-)
• Length: 176 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: B1EHP0
• Locus tag: AST80_RS17440
• Protein ID: WP_001277106.1
• Coordinates: 3560331..3560597 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AST80_RS17410	3554872..3555639	-	768	WP_044709901.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG	-
AST80_RS17415	3555636..3556913	-	1278	WP_044709902.1	branched chain amino acid ABC transporter permease LivM	-
AST80_RS17420	3556910..3557836	-	927	WP_001295111.1	high-affinity branched-chain amino acid ABC transporter permease LivH	-
AST80_RS17425	3557884..3558993	-	1110	WP_059235672.1	high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK	-
AST80_RS17430	3559417..3559800	+	384	WP_059235673.1	aspartate 1-decarboxylase autocleavage activator PanM	-
AST80_RS17435	3559797..3560324	-	528	WP_000342455.1	GNAT family N-acetyltransferase	Toxin
AST80_RS17440	3560331..3560597	-	267	WP_001277106.1	DUF1778 domain-containing protein	Antitoxin
AST80_RS17445	3560747..3561850	-	1104	WP_010319184.1	branched chain amino acid ABC transporter substrate-binding protein LivJ	-
AST80_RS17450	3562138..3562647	-	510	WP_059235675.1	hypothetical protein	-
AST80_RS17455	3562844..3563698	-	855	WP_000130217.1	RNA polymerase sigma factor RpoH	-
AST80_RS17460	3563943..3565001	-	1059	WP_001042000.1	permease-like cell division protein FtsX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (90-150)

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 176 a.a.
• Molecular weight: 19607.47 Da
• Isoelectric Point: 6.6343

>T30973 WP_000342455.1 NZ_AP014857:c3560324-3559797 [Escherichia albertii]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCSNTPERQVLGYYTLSGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNEKAHTFYQSLGFIPLVGENENAL
FFPTKSIELLFTQSD

Nucleotide

• Length: 528 bp

>T30973 NZ_AP014857:c3560324-3559797 [Escherichia albertii]
ATGGATGATCTGACGATAGAGATTCTGACCGATGATGCAGATTATGATCTACAGCGATTCGACTGCGGCGAGGAAGCGTT
AAATCTCTTTCTGACGACACATCTGGTTCGTCAACATCGCAATAAAATTTTGCGAGCGTATATCCTTTGTAGCAACACTC
CAGAACGTCAGGTCCTGGGATATTACACATTATCCGGCAGTTGTTTTGAACGAGCCGCACTGCCCTCGAAATCGAAACAG
AAAAAAATTCCCTACAAAAATATTCCCAGCGTCACTCTTGGGCGTCTGGCAATTGATCGTTCATTACAGGGGCAGGGATG
GGGAGCAACACTGGTTGCTCATGCCATGAAAGTCGTCTGGTCAGCCTCATTAGCGGTAGGTATTCACGGTCTTTTTGTCG
AGGCTCTGAATGAAAAAGCCCATACGTTTTATCAGTCGCTGGGCTTTATCCCGTTGGTTGGAGAAAACGAAAATGCGTTA
TTTTTCCCAACTAAATCCATTGAACTGCTTTTTACACAGAGCGATTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9942.37 Da
• Isoelectric Point: 10.0863

>AT30973 WP_001277106.1 NZ_AP014857:c3560597-3560331 [Escherichia albertii]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRMILNEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Nucleotide

• Length: 267 bp

>AT30973 NZ_AP014857:c3560597-3560331 [Escherichia albertii]
ATGTCTGCTGTTAAAAAGCAGCGTATCGATCTGCGTTTAACTGACGATGACAAAAGTATGATTGAGGAAGCGGCAGCGAT
ATCTAATCAATCCGTCAGCCAATTTATGTTGAACAGCGCTTCGCAGCGAGCTGCGGAAGTGATTGAACAGCATCGGCGGA
TGATTCTCAATGAGGAATCCTGGACGCGGGTGATGGATGCGCTGAGTAATCCACCGTCACCAGGTGAAAAGTTAAAACGT
GCGGCAAAACGTCTTCAGGGAATGTAA

Structures

Antitoxin

• AlphaFold DB: A0A7Z8E004