Detailed information of TA system TA030928

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataRT/DUF1778(antitoxin)
• Location: 3460846..3461646
• Replicon: chromosome
• Accession: NZ_AP014855
• Organism: Escherichia albertii strain NIAH_Bird_3

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: -
• Locus tag: AST93_RS17200
• Protein ID: WP_095574314.1
• Coordinates: 3460846..3461373 (-)
• Length: 176 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: B1EHP0
• Locus tag: AST93_RS17205
• Protein ID: WP_001277106.1
• Coordinates: 3461380..3461646 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AST93_RS17170	3455921..3456688	-	768	WP_059278385.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG	-
AST93_RS17175	3456685..3457962	-	1278	WP_059278386.1	branched chain amino acid ABC transporter permease LivM	-
AST93_RS17180	3457959..3458885	-	927	WP_001295111.1	high-affinity branched-chain amino acid ABC transporter permease LivH	-
AST93_RS17185	3458933..3460042	-	1110	WP_095574312.1	high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK	-
AST93_RS17195	3460466..3460849	+	384	WP_095574313.1	aspartate 1-decarboxylase autocleavage activator PanM	-
AST93_RS17200	3460846..3461373	-	528	WP_095574314.1	GNAT family N-acetyltransferase	Toxin
AST93_RS17205	3461380..3461646	-	267	WP_001277106.1	DUF1778 domain-containing protein	Antitoxin
AST93_RS17210	3461796..3462899	-	1104	WP_001021996.1	branched chain amino acid ABC transporter substrate-binding protein LivJ	-
AST93_RS17220	3463171..3464025	-	855	WP_000130217.1	RNA polymerase sigma factor RpoH	-
AST93_RS17225	3464270..3465328	-	1059	WP_001042000.1	permease-like cell division protein FtsX	-
AST93_RS17230	3465321..3465989	-	669	WP_000617720.1	cell division ATP-binding protein FtsE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (90-150)

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 176 a.a.
• Molecular weight: 19464.36 Da
• Isoelectric Point: 6.6263

>T30928 WP_095574314.1 NZ_AP014855:c3461373-3460846 [Escherichia albertii]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRACILCSNTPERQVLGYYTLSGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVCSASLAVGIHGLFVEALNEKAHTFYQSLGFIPLVGENENAL
FFPTKSIELLFTQSD

Nucleotide

• Length: 528 bp

>T30928 NZ_AP014855:c3461373-3460846 [Escherichia albertii]
ATGGATGATCTGACGATAGAGATTCTGACCGATGATGCAGATTATGATCTACAGCGATTCGACTGCGGCGAGGAAGCGTT
AAATCTCTTTCTGACGACACATCTGGTTCGTCAACATCGCAATAAAATTTTGCGAGCGTGTATCCTTTGTAGCAACACTC
CAGAACGTCAGGTCCTGGGATATTACACATTATCCGGCAGTTGTTTTGAACGAGCCGCACTGCCCTCGAAATCGAAACAG
AAAAAAATTCCCTACAAAAATATTCCCAGCGTCACTCTTGGGCGTCTGGCAATTGATCGTTCATTACAGGGGCAGGGATG
GGGAGCAACACTGGTTGCTCATGCCATGAAAGTCGTCTGCTCAGCCTCATTAGCGGTAGGTATTCACGGTCTTTTTGTCG
AGGCTCTGAATGAAAAAGCCCATACGTTTTATCAGTCGCTGGGCTTTATCCCGTTGGTTGGAGAAAACGAAAATGCGTTA
TTTTTCCCAACCAAATCCATTGAACTGCTTTTTACACAGAGCGATTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9942.37 Da
• Isoelectric Point: 10.0863

>AT30928 WP_001277106.1 NZ_AP014855:c3461646-3461380 [Escherichia albertii]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRMILNEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Nucleotide

• Length: 267 bp

>AT30928 NZ_AP014855:c3461646-3461380 [Escherichia albertii]
ATGTCTGCTGTTAAAAAGCAGCGTATCGATCTGCGTTTAACTGACGATGACAAAAGTATGATTGAGGAAGCGGCAGCGAT
ATCTAATCAATCCGTCAGCCAATTTATGTTGAACAGCGCTTCGCAGCGAGCTGCGGAAGTGATTGAACAGCATCGGCGGA
TGATTCTCAATGAGGAATCCTGGACGCGGGTGATGGATGCGCTGAGTAATCCACCGTCACCAGGTGAAAAGTTAAAACGT
GCGGCAAAACGTCTTCAGGGAATGTAA

Structures

Antitoxin

• AlphaFold DB: A0A7Z8E004