Detailed information of TA system TA030915

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-RelB
• Location: 1480889..1481493
• Replicon: chromosome
• Accession: NZ_AP014855
• Organism: Escherichia albertii strain NIAH_Bird_3

Toxin (Protein)

• Gene name: doc
• Uniprot ID: A0A8H9B4D1
• Locus tag: AST93_RS07145
• Protein ID: WP_059222236.1
• Coordinates: 1480889..1481275 (-)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: B1ELZ9
• Locus tag: AST93_RS07150
• Protein ID: WP_001195490.1
• Coordinates: 1481272..1481493 (-)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AST93_RS07145	1480889..1481275	-	387	WP_059222236.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
AST93_RS07150	1481272..1481493	-	222	WP_001195490.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
AST93_RS07155	1481911..1482669	+	759	WP_095573908.1	trans-aconitate 2-methyltransferase	-
AST93_RS07160	1482673..1483587	-	915	WP_024164792.1	bestrophin family protein	-
AST93_RS07165	1483792..1485243	-	1452	WP_095573909.1	tagaturonate reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (2-124)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-53)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14475.47 Da
• Isoelectric Point: 8.0833

>T30915 WP_059222236.1 NZ_AP014855:c1481275-1480889 [Escherichia albertii]
MIWVSAQEVIAFHDRILQRFPGVAGLADPGRAQALIYRVQNRVHYEGVTDLFELAATYWVAIARGHIFHDGNKRTAFFVT
MTFLWRNGIRIRDVDNSLENLTVEAATGEKTVGQLARCLRERVDSSGN

Nucleotide

• Length: 387 bp

>T30915 NZ_AP014855:c1481275-1480889 [Escherichia albertii]
ATGATTTGGGTCAGTGCACAGGAGGTCATTGCCTTCCACGATCGAATTTTGCAACGTTTTCCCGGTGTGGCGGGCCTGGC
CGACCCCGGCAGAGCACAGGCGCTGATTTATCGCGTGCAGAACCGTGTTCACTACGAGGGCGTTACTGACCTGTTTGAAC
TGGCAGCAACCTACTGGGTTGCCATCGCTCGCGGTCACATCTTTCATGATGGTAATAAACGTACCGCATTTTTCGTTACT
ATGACCTTTCTTTGGCGCAATGGCATCCGTATCCGTGACGTTGATAACTCGCTGGAGAACCTGACTGTCGAAGCGGCTAC
CGGAGAAAAAACGGTCGGTCAATTAGCGCGATGTTTACGTGAACGAGTGGATAGTTCCGGGAATTAA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8067.96 Da
• Isoelectric Point: 4.6816

>AT30915 WP_001195490.1 NZ_AP014855:c1481493-1481272 [Escherichia albertii]
MQTYTSTQARANIATVLDTALNGEPVEITRRDGSSAVLISKAAFEAYQNAKLDAEFDAIMQRHEHTIEALTDR

Nucleotide

• Length: 222 bp

>AT30915 NZ_AP014855:c1481493-1481272 [Escherichia albertii]
ATGCAAACTTACACTTCCACACAAGCCAGAGCCAATATTGCCACTGTACTGGATACCGCGTTGAATGGTGAGCCAGTTGA
AATCACCCGTCGCGACGGCAGCTCTGCCGTGCTCATTAGTAAAGCCGCATTCGAGGCGTATCAAAATGCGAAACTGGACG
CGGAATTTGATGCCATCATGCAACGTCATGAACACACAATTGAAGCGTTGACTGATCGATGA

Structures

Antitoxin

• AlphaFold DB: A0A2S6PD20