Detailed information of TA system TA030905

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 5321065..5321705
• Replicon: chromosome
• Accession: NZ_AP014839
• Organism: Pseudomonas aeruginosa strain 8380

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: PA8380_RS24690
• Protein ID: WP_003105740.1
• Coordinates: 5321065..5321475 (-)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: Q6X2S2
• Locus tag: PA8380_RS24695
• Protein ID: WP_003158175.1
• Coordinates: 5321475..5321705 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PA8380_RS24645	5316907..5317104	+	198	WP_003105756.1	hypothetical protein	-
PA8380_RS24650	5317260..5317988	+	729	WP_003105754.1	TIGR03761 family integrating conjugative element protein	-
PA8380_RS24655	5317994..5318542	+	549	WP_003105753.1	DUF3158 family protein	-
PA8380_RS24660	5318589..5319428	+	840	WP_003105750.1	Rha family transcriptional regulator	-
PA8380_RS24665	5319458..5319946	+	489	WP_003105748.1	single-stranded DNA-binding protein	-
PA8380_RS24670	5320054..5320299	+	246	WP_023102333.1	CrpP family protein	-
PA8380_RS24675	5320365..5320583	-	219	WP_003105747.1	hypothetical protein	-
PA8380_RS24685	5320783..5321043	-	261	WP_003105742.1	hypothetical protein	-
PA8380_RS24690	5321065..5321475	-	411	WP_003105740.1	tRNA(fMet)-specific endonuclease VapC	Toxin
PA8380_RS24695	5321475..5321705	-	231	WP_003158175.1	antitoxin	Antitoxin
PA8380_RS24700	5321961..5323880	+	1920	WP_004352838.1	type I DNA topoisomerase	-
PA8380_RS24705	5324188..5324397	+	210	WP_003105733.1	cold-shock protein	-
PA8380_RS24710	5324618..5326507	+	1890	WP_003105732.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	5301494..5407295	105801

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-124)

Antitoxin

• SpoVT-AbrB domain (7-35)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 15450.81 Da
• Isoelectric Point: 7.3233

>T30905 WP_003105740.1 NZ_AP014839:c5321475-5321065 [Pseudomonas aeruginosa]
MLKFMLDTNICIFTIKNRPQEVREAFLRHHDQMCISTVTLMELIYGAEKSSNPSRNLADVEGFAARLEVLKYDQEAAAHT
GQLRAELARLGKQIGPYDQMIAGHARSQGLIVVTNNRREFDRVPGLRVEDWVVPIV

Nucleotide

• Length: 411 bp

>T30905 NZ_AP014839:c5321475-5321065 [Pseudomonas aeruginosa]
ATGCTCAAGTTCATGCTCGACACCAACATCTGCATCTTCACCATCAAAAACCGCCCGCAAGAGGTGCGCGAGGCGTTCCT
GCGCCACCACGATCAGATGTGCATCAGCACCGTGACGCTGATGGAGCTGATCTACGGCGCAGAGAAGTCCTCGAACCCCT
CGCGCAATCTTGCAGACGTGGAAGGCTTCGCTGCTCGTCTGGAGGTGCTCAAGTACGACCAGGAAGCTGCCGCCCACACC
GGCCAGCTCCGCGCTGAACTGGCCCGCCTCGGCAAGCAAATCGGCCCCTATGACCAGATGATTGCCGGGCACGCCCGCTC
TCAGGGCCTGATCGTGGTCACCAATAACCGCCGCGAGTTCGACCGCGTACCCGGCTTGAGAGTTGAGGACTGGGTCGTCC
CAATCGTGTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8480.41 Da
• Isoelectric Point: 4.2821

>AT30905 WP_003158175.1 NZ_AP014839:c5321705-5321475 [Pseudomonas aeruginosa]
MEQGSVFQSNRSQAVRLPKAVALPDDVKRVDVVVVGRARIITPAGESWDSWFDGEGVTADFMAEREQPADQERETF

Nucleotide

• Length: 231 bp

>AT30905 NZ_AP014839:c5321705-5321475 [Pseudomonas aeruginosa]
ATGGAACAAGGTTCTGTTTTTCAGAGCAATCGTAGCCAGGCCGTAAGACTGCCCAAGGCAGTCGCGCTTCCCGATGACGT
TAAGCGTGTGGATGTGGTCGTTGTGGGGCGCGCCAGAATCATCACGCCTGCCGGCGAGTCTTGGGATAGCTGGTTCGATG
GCGAGGGCGTTACTGCCGACTTTATGGCAGAGCGTGAACAGCCTGCCGATCAGGAGCGCGAGACTTTCTGA

Structures

Antitoxin

• AlphaFold DB: Q6X2S2