Detailed information of TA system TA030901

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 2465194..2466236
• Replicon: chromosome
• Accession: NZ_AP014839
• Organism: Pseudomonas aeruginosa strain 8380

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: PA8380_RS11620
• Protein ID: WP_003109777.1
• Coordinates: 2465661..2466236 (+)
• Length: 192 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: I3TV68
• Locus tag: PA8380_RS11615
• Protein ID: WP_003050245.1
• Coordinates: 2465194..2465664 (+)
• Length: 157 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PA8380_RS11580	2460586..2462004	-	1419	WP_003109776.1	TIGR03752 family integrating conjugative element protein	-
PA8380_RS11585	2461994..2462905	-	912	WP_003105643.1	TIGR03749 family integrating conjugative element protein	-
PA8380_RS11590	2462902..2463594	-	693	WP_003105641.1	TIGR03746 family integrating conjugative element protein	-
PA8380_RS11595	2463591..2463989	-	399	WP_003105639.1	TIGR03750 family conjugal transfer protein	-
PA8380_RS11600	2464001..2464360	-	360	WP_003090173.1	TIGR03745 family integrating conjugative element membrane protein	-
PA8380_RS11605	2464377..2464610	-	234	WP_003050225.1	TIGR03758 family integrating conjugative element protein	-
PA8380_RS11610	2464607..2464990	-	384	WP_003105635.1	RAQPRD family integrative conjugative element protein	-
PA8380_RS11615	2465194..2465664	+	471	WP_003050245.1	helix-turn-helix domain-containing protein	Antitoxin
PA8380_RS11620	2465661..2466236	+	576	WP_003109777.1	PIN domain-containing protein	Toxin
PA8380_RS11625	2466254..2467168	+	915	WP_003105629.1	AAA family ATPase	-
PA8380_RS11630	2467165..2467635	+	471	WP_003105626.1	hypothetical protein	-
PA8380_RS11635	2467632..2468132	+	501	WP_003090159.1	hypothetical protein	-
PA8380_RS11640	2468132..2469034	+	903	WP_003105624.1	nucleotidyltransferase domain-containing protein	-
PA8380_RS11645	2469073..2469798	+	726	WP_003050273.1	CBASS effector endonuclease NucC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	2416751..2515044	98293

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (9-114)

Antitoxin

• Helix-turn-helix domain, group 17 (85-133)

Sequences

Toxin

Protein

• Length: 192 a.a.
• Molecular weight: 21644.79 Da
• Isoelectric Point: 5.6172

>T30901 WP_003109777.1 NZ_AP014839:2465661-2466236 [Pseudomonas aeruginosa]
MRHSPFTAVYDACVLYPAPLRDFLMWLGLSGRFRARWSQAIHEEWKRNLLINRPDLTRVQVDRTSDLMDRAIPDGLVEGY
EALVAGLTLPDPDDRHVLAAAIRCGASVIVTFNERDFPNDLLAPYGIESQHPDEFVDNLLDLDAAAVVSAAQRQRAQLKH
PPIEVDRYLEILLRQGLVQTTKVLATYRTIL

Nucleotide

• Length: 576 bp

>T30901 NZ_AP014839:2465661-2466236 [Pseudomonas aeruginosa]
ATGAGGCATTCCCCATTCACCGCCGTCTACGACGCGTGCGTTCTATATCCCGCGCCACTGCGGGATTTCCTGATGTGGCT
CGGCCTGTCTGGCCGCTTCCGGGCGCGGTGGAGCCAAGCCATTCATGAGGAGTGGAAACGCAACCTGTTGATCAACCGCC
CCGATCTCACCCGGGTTCAGGTCGACAGGACGTCGGATCTCATGGACCGGGCAATTCCGGACGGCTTGGTGGAGGGCTAC
GAAGCGCTCGTGGCAGGCCTGACATTGCCCGATCCGGACGACCGGCACGTCCTGGCTGCGGCGATTCGCTGCGGTGCGAG
CGTGATTGTGACGTTCAACGAACGGGATTTCCCGAACGATCTGCTGGCTCCGTACGGCATCGAATCGCAGCACCCCGATG
AGTTTGTGGACAACCTACTGGATCTGGACGCGGCCGCCGTGGTGTCGGCTGCGCAGCGCCAGCGTGCCCAACTCAAGCAT
CCGCCGATCGAGGTGGACCGCTATCTCGAAATCCTGCTGCGCCAGGGCCTTGTGCAAACGACCAAGGTGCTGGCGACCTA
TCGCACCATTCTCTGA

Antitoxin

Protein

• Length: 157 a.a.
• Molecular weight: 17245.62 Da
• Isoelectric Point: 6.3803

>AT30901 WP_003050245.1 NZ_AP014839:2465194-2465664 [Pseudomonas aeruginosa]
MTTATAQSKMTLPAAGEVKAAVQGQRALAAYLATQFETQHIQIFDDHKQAHQVELPTSALRLLVDILAELADGNAVKVVP
VHAELTTQEAADLLNVSRPHFVKLLEDGVLAFHRTGKHRRVRFADLMQYKEARERASEQAMAELAQQSQELGMGYE

Nucleotide

• Length: 471 bp

>AT30901 NZ_AP014839:2465194-2465664 [Pseudomonas aeruginosa]
ATGACCACCGCCACTGCCCAATCCAAGATGACCCTTCCCGCCGCGGGAGAGGTGAAGGCAGCCGTCCAGGGTCAACGTGC
CTTGGCGGCTTACCTCGCAACCCAGTTCGAGACGCAGCACATCCAGATCTTTGATGACCACAAACAGGCTCATCAGGTGG
AACTGCCTACCTCGGCTTTACGCCTGCTGGTCGACATCCTGGCCGAGTTGGCCGATGGCAATGCAGTAAAGGTGGTGCCC
GTCCATGCAGAGCTGACGACCCAAGAGGCGGCGGACTTGCTCAACGTGTCCCGTCCCCATTTCGTCAAGCTGCTCGAAGA
TGGGGTGTTGGCATTTCATCGCACCGGCAAGCACCGCAGGGTGAGGTTCGCCGATCTGATGCAGTACAAGGAAGCGCGCG
AGCGCGCCAGTGAGCAGGCGATGGCCGAACTCGCTCAGCAGTCGCAAGAGTTGGGAATGGGATACGAATGA

Structures

Antitoxin

• AlphaFold DB: I3TV68