Detailed information of TA system TA030895

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 12138..12751
• Replicon: plasmid pGM3709_03
• Accession: NZ_AP014824
• Organism: Geminocystis sp. NIES-3709

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A0D6AVP5
• Locus tag: GM3709_RS17935
• Protein ID: WP_066122176.1
• Coordinates: 12138..12524 (-)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: GM3709_RS17940
• Protein ID: WP_066122239.1
• Coordinates: 12521..12751 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GM3709_RS17910	7500..8633	+	1134	WP_066122162.1	reverse transcriptase family protein	-
GM3709_RS17915	8674..9462	+	789	WP_066122166.1	hypothetical protein	-
GM3709_RS17920	9489..11540	+	2052	WP_066122168.1	WYL domain-containing protein	-
GM3709_RS17925	11544..11909	-	366	WP_066122171.1	type II toxin-antitoxin system RelE/ParE family toxin	-
GM3709_RS17930	11899..12129	-	231	WP_144439494.1	DUF2281 domain-containing protein	-
GM3709_RS17935	12138..12524	-	387	WP_066122176.1	type II toxin-antitoxin system VapC family toxin	Toxin
GM3709_RS17940	12521..12751	-	231	WP_066122239.1	DUF2281 domain-containing protein	Antitoxin
GM3709_RS17945	12923..13147	-	225	WP_066122243.1	hypothetical protein	-
GM3709_RS17950	13471..14304	+	834	WP_066122179.1	hypothetical protein	-
GM3709_RS17955	14359..14700	+	342	WP_066122182.1	hypothetical protein	-
GM3709_RS17960	14858..15961	+	1104	WP_066122185.1	restriction endonuclease	-
GM3709_RS19405	15999..17744	+	1746	WP_071828126.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..49712	49712

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• VapC-like Sll0205 protein, PIN domain (5-127)

Antitoxin

• Domain of unknown function DUF2281 (6-74)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 15055.75 Da
• Isoelectric Point: 6.7203

>T30895 WP_066122176.1 NZ_AP014824:c12524-12138 [Geminocystis sp. NIES-3709]
MKGYLLDTHAFIWLSENDPNLPINLRQKIDSAEKVYLSIISLWEIAIKINIGKLSLKKSYQHIENELKLSDILLLPITFA
DTQRLCKLPLYHRDPFDRMLIIQAMERELILVSKDTKFSAYPIEILWE

Nucleotide

• Length: 387 bp

>T30895 NZ_AP014824:c12524-12138 [Geminocystis sp. NIES-3709]
ATGAAGGGTTATTTATTAGATACTCATGCTTTTATTTGGTTATCAGAAAATGATCCTAATTTGCCAATTAATCTAAGACA
AAAAATTGATTCAGCCGAAAAAGTTTATTTAAGTATTATTAGCCTTTGGGAGATTGCCATTAAAATCAATATCGGGAAAT
TATCCCTTAAAAAAAGTTATCAACATATTGAAAATGAACTAAAATTATCGGACATTCTCTTATTACCAATTACTTTTGCT
GACACTCAAAGACTATGCAAATTACCATTATATCATCGAGATCCTTTTGACAGAATGTTAATTATTCAGGCAATGGAAAG
AGAATTAATTTTAGTGAGTAAAGATACTAAATTTTCGGCTTATCCAATTGAAATATTATGGGAATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8936.07 Da
• Isoelectric Point: 4.2465

>AT30895 WP_066122239.1 NZ_AP014824:c12751-12521 [Geminocystis sp. NIES-3709]
INIETKILKTLEKMPVSLQEELLHYAEYLQEKYNQNNGKINASNQKLRSGILEGTFVLPLPDDFDESLEDFEEYMR

Nucleotide

• Length: 231 bp

>AT30895 NZ_AP014824:c12751-12521 [Geminocystis sp. NIES-3709]
ATAAATATTGAAACAAAAATATTGAAAACTTTAGAAAAAATGCCCGTATCTCTGCAAGAAGAATTATTGCATTATGCTGA
GTATCTTCAGGAAAAATATAATCAAAATAATGGAAAAATAAATGCTTCTAATCAAAAACTTCGCTCAGGCATTTTAGAAG
GCACTTTTGTTTTACCATTACCTGATGATTTTGATGAGTCTTTAGAAGATTTTGAGGAATACATGAGATGA

Structures

Toxin

• AlphaFold DB: A0A0D6AVP5