Detailed information of TA system TA030890

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/VagC-MazF
• Location: 1630274..1630849
• Replicon: chromosome
• Accession: NZ_AP014815
• Organism: Geminocystis sp. NIES-3708

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: GM3708_RS07135
• Protein ID: WP_066345196.1
• Coordinates: 1630274..1630531 (+)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A0D6AE68
• Locus tag: GM3708_RS07140
• Protein ID: WP_066345198.1
• Coordinates: 1630532..1630849 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GM3708_RS07110	1625294..1625962	+	669	WP_066345186.1	cytochrome b6	-
GM3708_RS07115	1626163..1626660	+	498	WP_066345188.1	cytochrome b6-f complex subunit IV	-
GM3708_RS07120	1627134..1627595	+	462	WP_066345190.1	DUF29 domain-containing protein	-
GM3708_RS07125	1627731..1629302	+	1572	WP_066345192.1	FkbM family methyltransferase	-
GM3708_RS07130	1629311..1630207	+	897	WP_066345194.1	FkbM family methyltransferase	-
GM3708_RS07135	1630274..1630531	+	258	WP_066345196.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Toxin
GM3708_RS07140	1630532..1630849	+	318	WP_066345198.1	type II toxin-antitoxin system PemK/MazF family toxin	Antitoxin
GM3708_RS07145	1630961..1632172	-	1212	WP_066345200.1	amidohydrolase	-
GM3708_RS07150	1632335..1633033	-	699	WP_066345201.1	pentapeptide repeat-containing protein	-
GM3708_RS07155	1633093..1633635	-	543	WP_066345202.1	16S rRNA (guanine(966)-N(2))-methyltransferase RsmD	-
GM3708_RS07160	1634045..1634818	-	774	WP_066345207.1	DUF1868 domain-containing protein	-
GM3708_RS07165	1634950..1635450	-	501	WP_066345208.1	cupin domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• SpoVT-AbrB domain (7-49)

Antitoxin

• mRNA interferase PemK-like (1-104)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 9962.36 Da
• Isoelectric Point: 5.0898

>T30890 WP_066345196.1 NZ_AP014815:1630274-1630531 [Geminocystis sp. NIES-3708]
MIKSHMVKIGNSQGVRLPKKLLELSGIKDKIYLSSENGKIIITPISKNRQDWDKVFKNMAENNDDQLLDINQDITCTWDE
QEWDW

Nucleotide

• Length: 258 bp

>T30890 NZ_AP014815:1630274-1630531 [Geminocystis sp. NIES-3708]
ATGATCAAATCCCATATGGTGAAAATAGGAAATTCTCAAGGTGTTAGATTACCGAAAAAGCTCCTAGAACTTTCCGGAAT
TAAAGACAAAATTTATTTATCTAGTGAGAATGGCAAAATTATAATTACTCCAATAAGCAAAAATCGTCAAGACTGGGATA
AGGTTTTTAAAAATATGGCTGAGAATAATGATGATCAACTTTTAGATATTAATCAGGATATTACATGCACTTGGGATGAA
CAAGAGTGGGATTGGTAG

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11985.15 Da
• Isoelectric Point: 8.4891

>AT30890 WP_066345198.1 NZ_AP014815:1630532-1630849 [Geminocystis sp. NIES-3708]
MKRFDIYLVVLNPTLGSEIKKTRPCVIISPDEMNDYINTIIIAPMTSTTKSYPTRVDCVFQEKKGQIVLDQIRTVEKIRL
IKKIGVLDSTHQEQVLSTLAEMFAL

Nucleotide

• Length: 318 bp

>AT30890 NZ_AP014815:1630532-1630849 [Geminocystis sp. NIES-3708]
ATGAAAAGATTTGATATTTATTTAGTTGTGCTTAATCCGACATTGGGAAGTGAAATCAAGAAAACTCGTCCTTGTGTAAT
TATTTCTCCTGATGAAATGAATGACTACATCAACACTATTATTATTGCACCAATGACCAGCACAACAAAATCTTATCCCA
CCCGTGTAGATTGTGTTTTTCAGGAAAAAAAAGGTCAAATAGTGTTAGATCAAATTAGAACAGTTGAGAAAATTCGTTTA
ATCAAAAAAATAGGAGTTCTCGATAGTACTCATCAAGAACAGGTTTTATCTACTTTAGCTGAAATGTTTGCACTGTAA

Structures

Antitoxin

• AlphaFold DB: A0A0D6AE68