Detailed information of TA system TA030877

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-VagC
• Location: 2966880..2967538
• Replicon: chromosome
• Accession: NZ_AP014704
• Organism: Methylobacterium aquaticum strain MA-22A

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A0C6FCQ3
• Locus tag: Maq22A_RS13580
• Protein ID: WP_060847420.1
• Coordinates: 2966880..2967284 (-)
• Length: 135 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: Maq22A_RS13585
• Protein ID: WP_099053403.1
• Coordinates: 2967281..2967538 (-)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Maq22A_RS13560	2963143..2964231	+	1089	WP_060849395.1	3,4-dihydroxy-2-butanone-4-phosphate synthase	-
Maq22A_RS13565	2964351..2965280	-	930	WP_060847417.1	histone deacetylase family protein	-
Maq22A_RS13570	2965477..2966076	+	600	WP_060847418.1	L,D-transpeptidase	-
Maq22A_RS13575	2966325..2966774	+	450	WP_060847419.1	hypothetical protein	-
Maq22A_RS13580	2966880..2967284	-	405	WP_060847420.1	type II toxin-antitoxin system VapC family toxin	Toxin
Maq22A_RS13585	2967281..2967538	-	258	WP_099053403.1	antitoxin	Antitoxin
Maq22A_RS13590	2967799..2968248	+	450	WP_060847422.1	hypothetical protein	-
Maq22A_RS13595	2968492..2970495	+	2004	WP_060847423.1	acetyl/propionyl/methylcrotonyl-CoA carboxylase subunit alpha	-
Maq22A_RS13600	2970513..2970929	+	417	WP_060847424.1	gamma-glutamylcyclotransferase	-
Maq22A_RS13605	2971258..2971680	-	423	WP_060847425.1	phosphoribosyl-AMP cyclohydrolase	-
Maq22A_RS13610	2971677..2972336	-	660	WP_060847426.1	GTP cyclohydrolase I FolE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-127)

Antitoxin

• SpoVT-AbrB domain (16-58)

Sequences

Toxin

Protein

• Length: 135 a.a.
• Molecular weight: 14204.46 Da
• Isoelectric Point: 5.1848

>T30877 WP_060847420.1 NZ_AP014704:c2967284-2966880 [Methylobacterium aquaticum]
MSGPRFLLDTSILSDLIRNPSGAVARQIARVGSKGIATSIVSVAELRYGCAKRGSARLLLQVEAVLEAIDVVPFEAPADV
IYGRIRADLEAAGRPIGPNDLLIAAQALALEVPLVTANEAEFRRVGGLTVENWL

Nucleotide

• Length: 405 bp

>T30877 NZ_AP014704:c2967284-2966880 [Methylobacterium aquaticum]
ATGAGCGGCCCACGCTTCCTGCTCGATACGAGCATCCTCTCCGATCTGATCCGCAACCCGTCCGGTGCCGTCGCCCGGCA
GATCGCCCGGGTCGGTAGCAAAGGCATCGCGACGAGCATCGTCTCGGTTGCGGAACTCCGCTACGGCTGCGCGAAGCGGG
GGTCGGCCCGCCTTCTCCTTCAGGTCGAAGCCGTCCTGGAGGCGATCGACGTGGTTCCGTTCGAGGCTCCCGCCGATGTC
ATCTACGGACGAATTCGCGCCGATCTCGAGGCTGCGGGTCGGCCGATCGGGCCGAACGATCTCCTCATCGCCGCTCAGGC
GCTCGCGCTCGAGGTTCCGCTCGTGACGGCGAACGAGGCGGAGTTTCGGCGCGTCGGCGGGCTGACGGTCGAGAACTGGC
TCTAA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9642.01 Da
• Isoelectric Point: 4.3115

>AT30877 WP_099053403.1 NZ_AP014704:c2967538-2967281 [Methylobacterium aquaticum]
MARPTPIPQPREVKLFRNNRSQAVRIPAEFALPGDRVRISRDGDRLILEPIGKKDLLEVLATLESIEEDFPDLDETLPPL
DDDPL

Nucleotide

• Length: 258 bp

>AT30877 NZ_AP014704:c2967538-2967281 [Methylobacterium aquaticum]
ATGGCGAGACCGACCCCGATACCGCAGCCGCGCGAGGTGAAGCTGTTCCGCAACAATCGGAGCCAGGCCGTGCGCATCCC
GGCCGAGTTCGCCTTGCCCGGAGACCGGGTCCGCATCAGCCGCGATGGCGACCGCCTCATCCTGGAGCCGATCGGCAAGA
AGGACCTTCTGGAGGTCCTGGCGACCCTGGAGTCGATCGAGGAGGATTTCCCGGACCTCGACGAGACGCTGCCGCCTCTG
GACGATGATCCGCTATGA

Structures

Toxin

• AlphaFold DB: A0A0C6FCQ3