Detailed information of TA system TA030846

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2094309..2094838
• Replicon: chromosome
• Accession: NZ_AP014652
• Organism: Staphylococcus aureus strain TMUS2126

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: SAJPND1_RS10555
• Protein ID: WP_000621175.1
• Coordinates: 2094309..2094671 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: SAJPND1_RS10560
• Protein ID: WP_000948331.1
• Coordinates: 2094668..2094838 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SAJPND1_RS10535	2091288..2092058	-	771	WP_001041102.1	RNA polymerase sigma factor SigB	-
SAJPND1_RS10540	2092033..2092512	-	480	WP_001190829.1	anti-sigma B factor RsbW	-
SAJPND1_RS10545	2092514..2092840	-	327	WP_001052491.1	anti-sigma factor antagonist	-
SAJPND1_RS10550	2092959..2093960	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
SAJPND1_RS10555	2094309..2094671	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
SAJPND1_RS10560	2094668..2094838	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
SAJPND1_RS10565	2094923..2096071	-	1149	WP_001281142.1	alanine racemase	-
SAJPND1_RS10570	2096137..2096496	-	360	WP_000581197.1	holo-ACP synthase	-
SAJPND1_RS10575	2096500..2096991	-	492	WP_078068808.1	PH domain-containing protein	-
SAJPND1_RS10580	2096978..2098561	-	1584	WP_001294626.1	PH domain-containing protein	-
SAJPND1_RS10585	2098554..2099033	-	480	WP_001287090.1	hypothetical protein	-
SAJPND1_RS10590	2099242..2099802	-	561	WP_001092402.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T30846 WP_000621175.1 NZ_AP014652:c2094671-2094309 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T30846 NZ_AP014652:c2094671-2094309 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT30846 WP_000948331.1 NZ_AP014652:c2094838-2094668 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT30846 NZ_AP014652:c2094838-2094668 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1