30837

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) PfiT-PfiA/ParE(toxin)
Location 5330125..5330733 Replicon chromosome
Accession NZ_AP014651
Organism Pseudomonas aeruginosa strain NCGM257

Toxin (Protein)


Gene name PfiT Uniprot ID A0A444LUU5
Locus tag PA257_RS25250 Protein ID WP_019486378.1
Coordinates 5330125..5330472 (-) Length 116 a.a.

Antitoxin (Protein)


Gene name PfiA Uniprot ID A0A0B0C355
Locus tag PA257_RS25255 Protein ID WP_003114155.1
Coordinates 5330482..5330733 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PA257_RS25230 5325151..5326182 + 1032 WP_003158906.1 AAA family ATPase -
PA257_RS25235 5326160..5328670 + 2511 WP_003160813.1 S8 family peptidase -
PA257_RS25240 5328769..5329074 - 306 WP_003158903.1 helix-turn-helix domain-containing protein -
PA257_RS25245 5329196..5329837 + 642 WP_003158902.1 substrate-binding domain-containing protein -
PA257_RS25250 5330125..5330472 - 348 WP_019486378.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
PA257_RS25255 5330482..5330733 - 252 WP_003114155.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
PA257_RS25260 5330947..5331930 - 984 WP_033990852.1 tyrosine-type recombinase/integrase -
PA257_RS25265 5331930..5333222 - 1293 WP_033990854.1 hypothetical protein -
PA257_RS25270 5333452..5334726 - 1275 WP_033990855.1 zonular occludens toxin family protein -
PA257_RS25275 5334730..5335086 - 357 WP_003114150.1 DUF2523 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element blaIMP-1 / floR / tet(G) / sul1 - 5233625..5342651 109026


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(6-100)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 116 a.a.        Molecular weight: 12956.72 Da        Isoelectric Point: 4.4212

>T30837 WP_019486378.1 NZ_AP014651:c5330472-5330125 [Pseudomonas aeruginosa]
MSPVVIRFTDTAEQSIEDQVHHLAPFQGEQAALQSVLSLLDEIEEKISLAPKGYPVSQQASLLGVLSYRELNTGPYRVFY
EFHEEQGEAAVILVLRQKQSVEQQLIRYCLVGPIE

Download         Length: 348 bp

>T30837 NZ_AP014651:c5330472-5330125 [Pseudomonas aeruginosa]
ATGTCCCCGGTCGTCATTCGTTTTACTGATACCGCAGAGCAAAGCATCGAAGACCAAGTCCACCACTTGGCTCCATTCCA
AGGTGAACAGGCTGCACTCCAGTCAGTACTGAGCCTTTTGGATGAGATTGAAGAGAAGATTTCACTTGCACCTAAAGGTT
ACCCAGTCAGCCAGCAGGCGAGTCTTCTGGGGGTGCTGAGCTATCGCGAGCTTAATACCGGCCCCTATCGTGTTTTTTAC
GAATTCCACGAAGAGCAAGGCGAGGCGGCAGTGATCTTGGTTTTGCGACAGAAGCAGAGCGTTGAGCAGCAATTGATCCG
CTACTGCTTGGTGGGGCCAATCGAGTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9442.79 Da        Isoelectric Point: 9.4808

>AT30837 WP_003114155.1 NZ_AP014651:c5330733-5330482 [Pseudomonas aeruginosa]
MRVETISYLKRHAADLDLSEPMVVTQNGVPAYVVESYAERKQRDEAIALVKLLAIGSRQYAEGKHRSVDDLKARLSRRFA
QPE

Download         Length: 252 bp

>AT30837 NZ_AP014651:c5330733-5330482 [Pseudomonas aeruginosa]
ATGCGAGTCGAGACAATTAGTTATTTGAAACGTCATGCGGCTGACCTGGATTTATCCGAGCCAATGGTCGTCACGCAGAA
CGGTGTTCCTGCCTATGTGGTTGAGTCATATGCTGAGCGGAAGCAGCGCGATGAAGCAATTGCGCTGGTGAAGTTGCTTG
CGATTGGCTCCCGCCAGTACGCAGAAGGCAAGCATCGCTCTGTTGATGATTTGAAAGCTCGCCTTTCCAGGAGGTTCGCT
CAGCCAGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A444LUU5

Antitoxin

Source ID Structure
AlphaFold DB A0A0B0C355

References