Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazE-MazF
Location 5548357..5548934 Replicon chromosome
Accession NZ_AP014642
Organism Leptolyngbya boryana dg5

Toxin (Protein)


Gene name mazF Uniprot ID A0A1Z4JCF0
Locus tag APT55_RS26350 Protein ID WP_017285932.1
Coordinates 5548357..5548620 (+) Length 88 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A1Z4JC99
Locus tag APT55_RS26355 Protein ID WP_017285931.1
Coordinates 5548608..5548934 (+) Length 109 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
APT55_RS26330 5543730..5543975 - 246 WP_017285936.1 DUF370 domain-containing protein -
APT55_RS26335 5544036..5545778 + 1743 WP_017285935.1 fibronectin-binding domain-containing protein -
APT55_RS26340 5546235..5547080 - 846 WP_017285934.1 3-deoxy-7-phosphoheptulonate synthase -
APT55_RS26345 5547432..5548232 - 801 WP_017285933.1 mechanosensitive ion channel family protein -
APT55_RS26350 5548357..5548620 + 264 WP_017285932.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Toxin
APT55_RS26355 5548608..5548934 + 327 WP_017285931.1 type II toxin-antitoxin system PemK/MazF family toxin Antitoxin
APT55_RS26360 5549119..5550123 - 1005 WP_017285930.1 GUN4 domain-containing protein -
APT55_RS26365 5550221..5550556 + 336 WP_017285929.1 nucleotidyltransferase family protein -
APT55_RS26370 5550546..5550890 + 345 WP_017285928.1 DUF86 domain-containing protein -
APT55_RS26375 5550940..5552217 - 1278 WP_017285927.1 adenosylhomocysteinase -
APT55_RS26380 5552620..5552958 + 339 WP_017285926.1 hypothetical protein -
APT55_RS26385 5553018..5553287 + 270 WP_017285925.1 hypothetical protein -
APT55_RS26390 5553376..5553750 + 375 WP_017285924.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

SpoVT-AbrB domain

(12-49)

Antitoxin

mRNA interferase PemK-like

(3-101)


Sequences

Toxin        


Download         Length: 88 a.a.        Molecular weight: 9789.91 Da        Isoelectric Point: 4.2815

>T30822 WP_017285932.1 NZ_AP014642:5548357-5548620 [Leptolyngbya boryana dg5]
MSGTIRTRLVKFGNSQGIRIPKLLLEQSGIQSEVEIEVEGNRLIIRPVSHPRAGWEAAIDAEIAKSGDEGLLDDETSTQW
DETEWQW

Download         Length: 264 bp

>T30822 NZ_AP014642:5548357-5548620 [Leptolyngbya boryana dg5]
ATGAGTGGAACAATTCGGACTCGACTTGTCAAATTTGGTAATTCTCAAGGAATTCGGATTCCGAAGCTGTTACTAGAGCA
AAGTGGCATTCAAAGTGAAGTTGAAATCGAAGTCGAAGGCAATCGCTTGATTATTCGTCCTGTATCTCATCCTCGTGCTG
GATGGGAAGCAGCGATCGATGCAGAAATCGCAAAATCTGGGGATGAAGGCTTGCTGGATGATGAAACCTCAACTCAATGG
GACGAAACCGAATGGCAATGGTAG

Antitoxin


Download         Length: 109 a.a.        Molecular weight: 12201.35 Da        Isoelectric Point: 8.5508

>AT30822 WP_017285931.1 NZ_AP014642:5548608-5548934 [Leptolyngbya boryana dg5]
MAMVVRRFDVFLVQLDPTVGSEIQKTRPCIIVSPDEMNRLLKTVLIAPLTSVRRNFPFRVDCQFQGKAGQIVLDQIRAVD
KTRLVQFLGQIGEEQRALLDTIADLFAA

Download         Length: 327 bp

>AT30822 NZ_AP014642:5548608-5548934 [Leptolyngbya boryana dg5]
ATGGCAATGGTAGTTCGTCGTTTTGATGTGTTTCTTGTTCAGCTAGATCCAACTGTTGGATCTGAAATTCAGAAGACCCG
TCCTTGTATAATCGTCTCTCCCGATGAGATGAATCGTTTACTCAAAACGGTTTTGATAGCTCCATTAACTTCTGTAAGAC
GAAACTTCCCATTTCGAGTTGATTGTCAGTTTCAAGGCAAAGCGGGTCAGATTGTCCTAGATCAAATTCGAGCAGTAGAT
AAAACCCGACTTGTTCAATTTCTGGGTCAAATTGGCGAGGAGCAAAGAGCGCTTCTCGATACGATAGCTGACCTCTTTGC
TGCCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Z4JCF0

Antitoxin

Source ID Structure
AlphaFold DB A0A1Z4JC99

References