Detailed information of TA system TA030820

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-MazE
• Location: 4558321..4558950
• Replicon: chromosome
• Accession: NZ_AP014642
• Organism: Leptolyngbya boryana dg5

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: APT55_RS21680
• Protein ID: WP_017286993.1
• Coordinates: 4558549..4558950 (+)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A1Z4JFE3
• Locus tag: APT55_RS21675
• Protein ID: WP_017286994.1
• Coordinates: 4558321..4558548 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
APT55_RS21645	4553515..4554747	+	1233	WP_017287001.1	Zn-dependent hydrolase	-
APT55_RS21650	4554950..4555795	+	846	WP_017287000.1	trypsin-like peptidase domain-containing protein	-
APT55_RS21655	4555895..4556155	+	261	WP_017286999.1	hypothetical protein	-
APT55_RS21660	4556237..4556566	+	330	WP_197693399.1	hypothetical protein	-
APT55_RS21665	4556892..4557269	+	378	WP_017286997.1	nuclear transport factor 2 family protein	-
APT55_RS32170	4557722..4558033	+	312	WP_144056170.1	hypothetical protein	-
APT55_RS21675	4558321..4558548	+	228	WP_017286994.1	virulence factor	Antitoxin
APT55_RS21680	4558549..4558950	+	402	WP_017286993.1	type II toxin-antitoxin system VapC family toxin	Toxin
APT55_RS21685	4558977..4559192	+	216	WP_197693400.1	nucleotidyltransferase domain-containing protein	-
APT55_RS21690	4559217..4560635	+	1419	WP_017286991.1	YdiU family protein	-
APT55_RS21695	4560642..4561208	+	567	WP_017286990.1	GNAT family N-acetyltransferase	-
APT55_RS21700	4561205..4562563	+	1359	WP_017286989.1	tRNA uridine-5-carboxymethylaminomethyl(34) synthesis GTPase MnmE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-124)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 14816.21 Da
• Isoelectric Point: 5.8399

>T30820 WP_017286993.1 NZ_AP014642:4558549-4558950 [Leptolyngbya boryana dg5]
MQYLLDTNICIYLIKQRPPKVLERFSNLALSDIGISSITVAELEYGVCKSQQREKNRNALMQFLIPLEIVAFDQAAATLY
GAIRSDLESRGLVIGAMDMLIAAHALSLSVTLVSNNVREFSRIANLSLQNWAE

Nucleotide

• Length: 402 bp

>T30820 NZ_AP014642:4558549-4558950 [Leptolyngbya boryana dg5]
ATGCAATATCTGTTGGACACGAACATCTGCATCTATTTGATTAAGCAAAGACCTCCAAAGGTATTGGAGCGATTTTCAAA
TCTTGCTTTGTCAGATATCGGCATATCCTCTATCACTGTGGCGGAACTAGAATATGGCGTTTGCAAAAGCCAGCAACGAG
AGAAAAACCGTAATGCTTTGATGCAATTTTTAATCCCGCTAGAAATCGTTGCGTTTGATCAAGCGGCTGCGACCCTCTAT
GGCGCAATCAGAAGCGATCTAGAAAGTAGAGGACTGGTTATCGGAGCAATGGACATGCTGATTGCTGCCCACGCATTGAG
CCTTAGCGTTACGTTAGTCAGCAACAATGTGCGAGAGTTTTCACGCATTGCCAATCTATCTCTGCAAAACTGGGCAGAAT
GA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8511.74 Da
• Isoelectric Point: 4.5007

>AT30820 WP_017286994.1 NZ_AP014642:4558321-4558548 [Leptolyngbya boryana dg5]
MNIAKLSTDGTHQIVILPEDCKMTGTEVYIKKVGSAIVLIAKDHPWQSLFDSLEQFSEDFMTTREQPPLDVREAF

Nucleotide

• Length: 228 bp

>AT30820 NZ_AP014642:4558321-4558548 [Leptolyngbya boryana dg5]
ATGAATATTGCCAAACTTAGCACCGATGGCACCCATCAAATTGTCATACTCCCCGAAGACTGCAAAATGACGGGTACGGA
GGTCTACATTAAAAAAGTTGGAAGTGCGATCGTTCTAATTGCCAAAGATCATCCTTGGCAATCGCTCTTCGATAGCTTAG
AGCAATTCTCTGAAGACTTCATGACCACGAGAGAGCAGCCCCCTCTCGACGTTCGTGAGGCTTTCTAA

Structures

Antitoxin

• AlphaFold DB: A0A1Z4JFE3