Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazE-MazF
Location 5548356..5548933 Replicon chromosome
Accession NZ_AP014638
Organism Leptolyngbya boryana IAM M-101

Toxin (Protein)


Gene name mazF Uniprot ID A0A1Z4JCF0
Locus tag LBWT_RS26160 Protein ID WP_017285932.1
Coordinates 5548356..5548619 (+) Length 88 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A1Z4JC99
Locus tag LBWT_RS26165 Protein ID WP_017285931.1
Coordinates 5548607..5548933 (+) Length 109 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LBWT_RS26140 5543729..5543974 - 246 WP_017285936.1 DUF370 domain-containing protein -
LBWT_RS26145 5544035..5545777 + 1743 WP_017285935.1 fibronectin-binding domain-containing protein -
LBWT_RS26150 5546234..5547079 - 846 WP_017285934.1 3-deoxy-7-phosphoheptulonate synthase -
LBWT_RS26155 5547431..5548231 - 801 WP_017285933.1 mechanosensitive ion channel family protein -
LBWT_RS26160 5548356..5548619 + 264 WP_017285932.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Toxin
LBWT_RS26165 5548607..5548933 + 327 WP_017285931.1 type II toxin-antitoxin system PemK/MazF family toxin Antitoxin
LBWT_RS26170 5549118..5550122 - 1005 WP_017285930.1 GUN4 domain-containing protein -
LBWT_RS26175 5550220..5550555 + 336 WP_017285929.1 nucleotidyltransferase family protein -
LBWT_RS26180 5550545..5550889 + 345 WP_017285928.1 DUF86 domain-containing protein -
LBWT_RS26185 5550939..5552216 - 1278 WP_017285927.1 adenosylhomocysteinase -
LBWT_RS26190 5552619..5552957 + 339 WP_017285926.1 hypothetical protein -
LBWT_RS26195 5553017..5553286 + 270 WP_017285925.1 hypothetical protein -
LBWT_RS26200 5553375..5553749 + 375 WP_017285924.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

SpoVT-AbrB domain

(12-49)

Antitoxin

mRNA interferase PemK-like

(3-101)


Sequences

Toxin        


Download         Length: 88 a.a.        Molecular weight: 9789.91 Da        Isoelectric Point: 4.2815

>T30817 WP_017285932.1 NZ_AP014638:5548356-5548619 [Leptolyngbya boryana IAM M-101]
MSGTIRTRLVKFGNSQGIRIPKLLLEQSGIQSEVEIEVEGNRLIIRPVSHPRAGWEAAIDAEIAKSGDEGLLDDETSTQW
DETEWQW

Download         Length: 264 bp

>T30817 NZ_AP014638:5548356-5548619 [Leptolyngbya boryana IAM M-101]
ATGAGTGGAACAATTCGGACTCGACTTGTCAAATTTGGTAATTCTCAAGGAATTCGGATTCCGAAGCTGTTACTAGAGCA
AAGTGGCATTCAAAGTGAAGTTGAAATCGAAGTCGAAGGCAATCGCTTGATTATTCGTCCTGTATCTCATCCTCGTGCTG
GATGGGAAGCAGCGATCGATGCAGAAATCGCAAAATCTGGGGATGAAGGCTTGCTGGATGATGAAACCTCAACTCAATGG
GACGAAACCGAATGGCAATGGTAG

Antitoxin


Download         Length: 109 a.a.        Molecular weight: 12201.35 Da        Isoelectric Point: 8.5508

>AT30817 WP_017285931.1 NZ_AP014638:5548607-5548933 [Leptolyngbya boryana IAM M-101]
MAMVVRRFDVFLVQLDPTVGSEIQKTRPCIIVSPDEMNRLLKTVLIAPLTSVRRNFPFRVDCQFQGKAGQIVLDQIRAVD
KTRLVQFLGQIGEEQRALLDTIADLFAA

Download         Length: 327 bp

>AT30817 NZ_AP014638:5548607-5548933 [Leptolyngbya boryana IAM M-101]
ATGGCAATGGTAGTTCGTCGTTTTGATGTGTTTCTTGTTCAGCTAGATCCAACTGTTGGATCTGAAATTCAGAAGACCCG
TCCTTGTATAATCGTCTCTCCCGATGAGATGAATCGTTTACTCAAAACGGTTTTGATAGCTCCATTAACTTCTGTAAGAC
GAAACTTCCCATTTCGAGTTGATTGTCAGTTTCAAGGCAAAGCGGGTCAGATTGTCCTAGATCAAATTCGAGCAGTAGAT
AAAACCCGACTTGTTCAATTTCTGGGTCAAATTGGCGAGGAGCAAAGAGCGCTTCTCGATACGATAGCTGACCTCTTTGC
TGCCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Z4JCF0

Antitoxin

Source ID Structure
AlphaFold DB A0A1Z4JC99

References