Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/DUF433(antitoxin)
Location 4796350..4796912 Replicon chromosome
Accession NZ_AP014638
Organism Leptolyngbya boryana IAM M-101

Toxin (Protein)


Gene name vapC Uniprot ID A0A1Z4JEA4
Locus tag LBWT_RS22795 Protein ID WP_017286620.1
Coordinates 4796350..4796691 (-) Length 114 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A1Z4JEC0
Locus tag LBWT_RS22800 Protein ID WP_017286619.1
Coordinates 4796688..4796912 (-) Length 75 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LBWT_RS22770 4791517..4792227 + 711 WP_017286625.1 ABC transporter ATP-binding protein -
LBWT_RS22775 4792292..4793476 + 1185 WP_017286624.1 ABC transporter substrate-binding protein -
LBWT_RS22780 4793518..4794468 + 951 WP_017286623.1 branched-chain amino acid ABC transporter permease -
LBWT_RS22785 4794465..4795391 + 927 WP_017286622.1 branched-chain amino acid ABC transporter permease -
LBWT_RS22790 4795484..4796305 + 822 WP_017286621.1 slipin family protein -
LBWT_RS22795 4796350..4796691 - 342 WP_017286620.1 DUF5615 family PIN-like protein Toxin
LBWT_RS22800 4796688..4796912 - 225 WP_017286619.1 DUF433 domain-containing protein Antitoxin
LBWT_RS22805 4797123..4797809 + 687 WP_017286618.1 SEC59/DGK1/VTE5 family protein -
LBWT_RS22810 4797859..4799493 + 1635 WP_017286617.1 ABC transporter ATP-binding protein -
LBWT_RS22815 4799559..4800248 + 690 WP_017286616.1 glycoside hydrolase family protein -
LBWT_RS32060 4800654..4800818 + 165 WP_017286615.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Domain of unknown function DUF5615

(4-79)

Antitoxin

Protein of unknown function DUF433

(8-63)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 12378.18 Da        Isoelectric Point: 4.1314

>T30816 WP_017286620.1 NZ_AP014638:c4796691-4796350 [Leptolyngbya boryana IAM M-101]
VTTIWIDAHLSPVIATWITGRFGITAIALRDIGLRDAEDPEIFEAAKAQGVIFMTKDSDFVDLVERLEAPPQIIWLTCGN
TSNARLQEILSSTLPDALELLKSGEKLVEISGE

Download         Length: 342 bp

>T30816 NZ_AP014638:c4796691-4796350 [Leptolyngbya boryana IAM M-101]
GTGACAACGATTTGGATCGATGCCCATTTGTCGCCTGTGATCGCAACTTGGATTACAGGCAGATTTGGAATTACCGCGAT
CGCTTTACGGGATATCGGCTTGAGAGATGCCGAAGATCCCGAAATCTTTGAGGCTGCAAAAGCTCAAGGCGTTATATTCA
TGACTAAAGATAGCGATTTCGTGGATCTCGTTGAACGTTTGGAAGCACCGCCACAAATTATTTGGTTAACTTGTGGCAAT
ACATCAAATGCCCGTTTGCAGGAAATTCTAAGCTCGACTTTGCCTGATGCATTAGAGTTATTGAAATCTGGCGAAAAGCT
AGTAGAGATTAGCGGAGAGTAA

Antitoxin


Download         Length: 75 a.a.        Molecular weight: 8186.57 Da        Isoelectric Point: 7.8330

>AT30816 WP_017286619.1 NZ_AP014638:c4796912-4796688 [Leptolyngbya boryana IAM M-101]
MSDLLKRITVNPKQCGGRPCIRGMRIRVSDVLDLFAAGLSAEQILEELPDLERDDLRAALAYASRKVNHPVLVA

Download         Length: 225 bp

>AT30816 NZ_AP014638:c4796912-4796688 [Leptolyngbya boryana IAM M-101]
ATGTCAGACTTACTTAAACGAATCACCGTGAATCCTAAGCAATGTGGGGGTCGCCCCTGTATTCGTGGCATGAGAATTCG
AGTGTCCGATGTTTTGGATTTATTTGCAGCAGGGTTAAGTGCTGAACAAATTTTGGAAGAACTGCCTGATCTGGAAAGAG
ATGATTTAAGAGCAGCACTTGCCTACGCTTCACGTAAAGTCAACCACCCTGTTTTAGTCGCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Z4JEA4

Antitoxin

Source ID Structure
AlphaFold DB A0A1Z4JEC0

References