Detailed information of TA system TA030814

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 3347391..3348427
• Replicon: chromosome
• Accession: NZ_AP014638
• Organism: Leptolyngbya boryana IAM M-101

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: LBWT_RS15665
• Protein ID: WP_017288215.1
• Coordinates: 3347861..3348427 (+)
• Length: 189 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: A0A1Z4JIQ6
• Locus tag: LBWT_RS15660
• Protein ID: WP_017288216.1
• Coordinates: 3347391..3347855 (+)
• Length: 155 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LBWT_RS15645	3343619..3346327	+	2709	WP_017288219.1	type I DNA topoisomerase	-
LBWT_RS15655	3346804..3347007	+	204	WP_081614741.1	hypothetical protein	-
LBWT_RS31925	3347142..3347297	+	156	WP_154660829.1	hypothetical protein	-
LBWT_RS15660	3347391..3347855	+	465	WP_017288216.1	helix-turn-helix domain-containing protein	Antitoxin
LBWT_RS15665	3347861..3348427	+	567	WP_017288215.1	PIN domain-containing protein	Toxin
LBWT_RS15670	3348800..3349432	+	633	WP_017288213.1	hypothetical protein	-
LBWT_RS15675	3349485..3349694	-	210	WP_144056198.1	hypothetical protein	-
LBWT_RS15680	3349732..3350082	+	351	WP_017288212.1	hypothetical protein	-
LBWT_RS15690	3350576..3351673	+	1098	WP_017288211.1	PAS domain-containing protein	-
LBWT_RS15695	3353077..3353406	-	330	WP_017288210.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-113)

Antitoxin

• SinI-like, DNA-binding domain (83-128)

Sequences

Toxin

Protein

• Length: 189 a.a.
• Molecular weight: 21641.89 Da
• Isoelectric Point: 5.6857

>T30814 WP_017288215.1 NZ_AP014638:3347861-3348427 [Leptolyngbya boryana IAM M-101]
MSNFTAVYDACVLYPAPLRDFLMELALTDLFRARWSESIHQEWIRNVLKSRPDLSAEQLNKTKELMNSHVRDSVVTNYET
LIPAIELPDPDDRHVLAAAIRCNASVIVTFNLKDFPADTLKLYDIDAQHPDEFILYLLDLKPLAVCTAAERQRQRLKKPP
KDPEEYLGTLLRQGLPQTVSRLRESCYL

Nucleotide

• Length: 567 bp

>T30814 NZ_AP014638:3347861-3348427 [Leptolyngbya boryana IAM M-101]
ATGTCTAATTTCACGGCTGTTTACGATGCGTGTGTTCTCTATCCTGCTCCACTACGCGACTTTTTGATGGAACTTGCTCT
AACTGACTTATTTAGAGCAAGGTGGTCAGAATCTATTCACCAAGAATGGATTCGGAATGTTCTTAAGAGCCGTCCTGATC
TTTCGGCTGAGCAACTTAATAAGACTAAGGAATTAATGAACAGTCATGTTCGAGATTCAGTAGTGACGAATTATGAAACA
TTAATTCCAGCGATTGAGCTTCCCGACCCGGATGATCGTCATGTGCTTGCTGCTGCTATTCGCTGTAACGCCAGTGTCAT
TGTCACGTTTAATTTGAAAGACTTTCCCGCAGACACGTTAAAGCTATATGACATTGATGCACAGCATCCAGATGAGTTCA
TTCTATATCTTCTAGATTTGAAGCCTCTGGCGGTTTGCACAGCAGCAGAGAGGCAGCGCCAACGCCTTAAAAAACCTCCA
AAAGATCCAGAAGAATATTTAGGAACTTTGCTTCGACAGGGACTACCTCAAACAGTTTCTCGCTTGCGAGAATCGTGTTA
TCTCTGA

Antitoxin

Protein

• Length: 155 a.a.
• Molecular weight: 17200.86 Da
• Isoelectric Point: 6.2467

>AT30814 WP_017288216.1 NZ_AP014638:3347391-3347855 [Leptolyngbya boryana IAM M-101]
MTITLQKSSPPTLEDIRLAKESSSRLARSIPSHESTSKISVIQGEATGETIVLPTAALQMLVEILAQMAQGNIVTLFPIH
AELTTQEAADLLKVSRPYLVKLLEDKALPFRKVGKHRRILFQDLMEYKNREDSLRSNALDELVAQAQELNMGYE

Nucleotide

• Length: 465 bp

>AT30814 NZ_AP014638:3347391-3347855 [Leptolyngbya boryana IAM M-101]
ATGACAATTACATTGCAGAAGTCCAGTCCTCCGACCCTAGAAGATATCCGTTTGGCGAAAGAAAGCAGCAGTAGGCTTGC
TCGATCCATTCCAAGTCATGAATCGACGAGTAAGATCAGCGTGATCCAGGGCGAGGCAACTGGTGAGACGATCGTATTGC
CGACAGCAGCGCTTCAGATGTTGGTTGAAATCCTTGCGCAGATGGCGCAGGGGAATATTGTTACTCTTTTTCCAATCCAC
GCAGAGCTTACAACTCAGGAAGCGGCTGATTTACTCAAAGTGTCCCGACCTTATCTAGTTAAGCTTCTTGAGGACAAGGC
ATTGCCTTTTAGAAAAGTTGGAAAGCATCGGCGCATTCTTTTCCAAGATCTTATGGAATACAAAAACCGTGAAGACAGCC
TTAGAAGTAATGCGCTGGATGAGCTTGTAGCTCAAGCCCAAGAGCTAAACATGGGCTATGAGTAA

Structures

Antitoxin

• AlphaFold DB: A0A1Z4JIQ6