Detailed information of TA system TA030812

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 5980134..5980795
• Replicon: chromosome
• Accession: NZ_AP014637
• Organism: Pseudomonas sp. StFLB209

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: PSCI_RS26540
• Protein ID: WP_045492837.1
• Coordinates: 5980388..5980795 (+)
• Length: 136 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A077LPQ7
• Locus tag: PSCI_RS26535
• Protein ID: WP_045492834.1
• Coordinates: 5980134..5980376 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PSCI_RS26525	5975587..5976375	+	789	WP_045492831.1	substrate-binding domain-containing protein	-
PSCI_RS26530	5976513..5979710	-	3198	WP_084710149.1	glycosyltransferase	-
PSCI_RS26535	5980134..5980376	+	243	WP_045492834.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
PSCI_RS26540	5980388..5980795	+	408	WP_045492837.1	type II toxin-antitoxin system VapC family toxin	Toxin
PSCI_RS26545	5980970..5981791	+	822	WP_045492840.1	NAD(P)-dependent oxidoreductase	-
PSCI_RS26550	5981793..5982683	+	891	WP_045492843.1	SMP-30/gluconolactonase/LRE family protein	-
PSCI_RS26555	5982749..5983357	+	609	WP_084710151.1	sigma-70 family RNA polymerase sigma factor	-
PSCI_RS26560	5983357..5984010	+	654	WP_045492846.1	ChrR family anti-sigma-E factor	-
PSCI_RS26565	5984497..5985009	+	513	Protein_5283	transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	5984611..5985009	398

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 136 a.a.
• Molecular weight: 15034.53 Da
• Isoelectric Point: 8.5149

>T30812 WP_045492837.1 NZ_AP014637:5980388-5980795 [Pseudomonas sp. StFLB209]
MSPLYMLDTNMVSHIIKGTYPGVQKHLLNTPMDHICISAVTLGELRYGLAKRPDATRLHALVQAFLVRIDVLPWDSHVAN
CYGDLRASMERVGKPLGNLDMMIAAHAKATNVALISNDQAFRQIKGLHTLDWTKD

Nucleotide

• Length: 408 bp

>T30812 NZ_AP014637:5980388-5980795 [Pseudomonas sp. StFLB209]
ATGAGTCCGCTGTATATGCTCGATACAAACATGGTCAGCCATATCATCAAGGGCACTTACCCTGGCGTGCAGAAGCATCT
GCTCAATACGCCCATGGATCACATATGCATCTCGGCGGTTACTCTGGGTGAATTACGCTATGGCCTGGCCAAGCGACCTG
ACGCGACGCGCCTGCACGCACTCGTGCAGGCGTTTCTGGTTCGCATAGATGTTTTGCCTTGGGACAGCCATGTCGCCAAC
TGCTATGGCGACCTGCGGGCCTCTATGGAACGCGTTGGCAAACCGCTCGGCAATCTGGACATGATGATTGCCGCTCATGC
CAAAGCGACCAATGTTGCGCTGATCAGCAATGACCAGGCATTCAGACAGATCAAGGGTCTGCATACGCTCGACTGGACCA
AGGATTAG

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9311.50 Da
• Isoelectric Point: 6.4564

>AT30812 WP_045492834.1 NZ_AP014637:5980134-5980376 [Pseudomonas sp. StFLB209]
MHHVAKLFNNGRSQAVRLPASFRFDCKEVYIRQDPTTGDVILSRKPGTWDGFFELLKDSKVPDDFMSDRQDLPPQERDPF

Nucleotide

• Length: 243 bp

>AT30812 NZ_AP014637:5980134-5980376 [Pseudomonas sp. StFLB209]
ATGCACCACGTTGCCAAACTGTTCAACAACGGCAGAAGTCAGGCTGTGCGTCTCCCGGCGTCGTTTCGATTCGATTGCAA
GGAAGTCTATATCCGCCAGGACCCCACAACAGGTGACGTCATTCTTTCGCGAAAGCCGGGTACCTGGGACGGTTTTTTTG
AACTCTTGAAAGACAGCAAGGTGCCCGACGACTTCATGTCTGATCGCCAGGATCTGCCACCTCAAGAACGCGATCCATTT
TGA

Structures

Antitoxin

• AlphaFold DB: A0A077LPQ7