Detailed information of TA system TA030810

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/upstrm_HI1419-dnstrm_HI1420
• Location: 4490157..4490746
• Replicon: chromosome
• Accession: NZ_AP014637
• Organism: Pseudomonas sp. StFLB209

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: -
• Locus tag: PSCI_RS20195
• Protein ID: WP_045494637.1
• Coordinates: 4490447..4490746 (-)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: A0A077LKN5
• Locus tag: PSCI_RS20190
• Protein ID: WP_045490500.1
• Coordinates: 4490157..4490450 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PSCI_RS20170	4485585..4486763	-	1179	WP_045490488.1	MFS transporter	-
PSCI_RS20175	4486922..4488043	+	1122	WP_045490490.1	lactonase family protein	-
PSCI_RS20180	4488117..4489706	-	1590	WP_084710042.1	ABC-F family ATPase	-
PSCI_RS20190	4490157..4490450	-	294	WP_045490500.1	putative addiction module antidote protein	Antitoxin
PSCI_RS20195	4490447..4490746	-	300	WP_045494637.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
PSCI_RS20200	4491180..4491587	+	408	WP_052483442.1	hypothetical protein	-
PSCI_RS29185	4491596..4491856	+	261	WP_144403291.1	hypothetical protein	-
PSCI_RS29550	4492273..4492422	+	150	WP_173426693.1	hypothetical protein	-
PSCI_RS20205	4492455..4492709	-	255	WP_045494643.1	hypothetical protein	-
PSCI_RS20210	4493014..4493163	+	150	WP_045490502.1	type B 50S ribosomal protein L36	-
PSCI_RS20215	4493525..4493842	-	318	WP_045490504.1	multidrug transporter	-
PSCI_RS20220	4494067..4494510	+	444	WP_045490506.1	hemerythrin domain-containing protein	-
PSCI_RS20225	4494561..4494908	+	348	WP_045490508.1	hypothetical protein	-
PSCI_RS20230	4494917..4495192	-	276	WP_045490510.1	peptidylprolyl isomerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (41-91)

Antitoxin

• Uncharacterized protein HI1420 (5-91)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 10819.44 Da
• Isoelectric Point: 10.9911

>T30810 WP_045494637.1 NZ_AP014637:c4490746-4490447 [Pseudomonas sp. StFLB209]
MTYEIRQTRSFAHWHASLRDLGARIAVARRIERAAAGNLGDVKSVGEGISELRVHVGAGYRVYFTTRNQTLIVLLIGGSK
STQSADIKQAKALAKEVLP

Nucleotide

• Length: 300 bp

>T30810 NZ_AP014637:c4490746-4490447 [Pseudomonas sp. StFLB209]
ATGACTTATGAAATTCGACAAACCAGATCGTTTGCCCACTGGCATGCATCCTTGCGCGACTTGGGTGCCAGAATCGCTGT
TGCACGTCGCATTGAAAGAGCCGCAGCAGGCAATCTGGGTGATGTGAAAAGCGTAGGCGAAGGTATTTCGGAGCTACGTG
TACATGTTGGCGCGGGCTACCGGGTCTATTTCACAACACGCAACCAGACACTCATCGTGCTACTGATCGGGGGTAGCAAA
TCAACCCAGTCTGCCGACATAAAGCAGGCGAAAGCTTTGGCAAAGGAGGTCCTGCCATGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10522.24 Da
• Isoelectric Point: 6.2159

>AT30810 WP_045490500.1 NZ_AP014637:c4490450-4490157 [Pseudomonas sp. StFLB209]
MTDTLLPFDMAALLDSDEAITEYLTQVLADGDTDELIRAVSHVAKARGMAQIAKDSGLGRASLYKALAPGAKPRFDTIMK
VMRALGIELHVRPMTHP

Nucleotide

• Length: 294 bp

>AT30810 NZ_AP014637:c4490450-4490157 [Pseudomonas sp. StFLB209]
ATGACCGATACCCTGTTGCCGTTCGATATGGCGGCACTGCTCGACAGTGATGAGGCAATCACCGAGTACCTGACCCAGGT
GTTGGCCGATGGCGACACCGACGAACTCATCAGAGCCGTTTCCCATGTGGCCAAAGCCCGAGGCATGGCGCAAATTGCCA
AAGATTCCGGGCTTGGCAGGGCCAGTCTTTACAAGGCCCTCGCTCCTGGAGCCAAACCACGCTTTGACACCATCATGAAA
GTGATGCGCGCCCTCGGCATCGAACTTCATGTCAGACCCATGACACATCCTTGA

Structures

Antitoxin

• AlphaFold DB: A0A077LKN5