Detailed information of TA system TA030796

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PfiT-PfiA/-
• Location: 6700082..6700687
• Replicon: chromosome
• Accession: NZ_AP014628
• Organism: Pseudomonas sp. St29

Toxin (Protein)

• Gene name: PfiT
• Uniprot ID: -
• Locus tag: PST29_RS30010
• Protein ID: WP_060844990.1
• Coordinates: 6700082..6700426 (-)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: PfiA
• Uniprot ID: A0A7Y1CEW2
• Locus tag: PST29_RS30015
• Protein ID: WP_025131512.1
• Coordinates: 6700439..6700687 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PST29_RS29990	6695407..6696489	-	1083	WP_060841770.1	5-(carboxyamino)imidazole ribonucleotide synthase	-
PST29_RS29995	6696501..6696992	-	492	WP_016967060.1	5-(carboxyamino)imidazole ribonucleotide mutase	-
PST29_RS30000	6697408..6698322	-	915	WP_060841771.1	LysR family transcriptional regulator	-
PST29_RS30005	6698504..6699928	+	1425	WP_060842033.1	aspartate ammonia-lyase	-
PST29_RS30010	6700082..6700426	-	345	WP_060844990.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
PST29_RS30015	6700439..6700687	-	249	WP_025131512.1	prevent-host-death protein	Antitoxin
PST29_RS30020	6701291..6701644	+	354	WP_060844991.1	DUF3077 domain-containing protein	-
PST29_RS30025	6702111..6702836	+	726	WP_060845190.1	hypothetical protein	-
PST29_RS30030	6702935..6703579	+	645	WP_060844992.1	hypothetical protein	-
PST29_RS30035	6703616..6704635	+	1020	WP_060844993.1	beta-ketoacyl-ACP synthase 3	-
PST29_RS30040	6704635..6705642	+	1008	WP_060844994.1	NAD(P)-dependent oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-99)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 12868.57 Da
• Isoelectric Point: 4.2876

>T30796 WP_060844990.1 NZ_AP014628:c6700426-6700082 [Pseudomonas sp. St29]
MPTLTIRYTETAQQSIEDQIVHLADYSDLKSAFERLDAVIDALQDKLLSAPLGYPVSPQASDLGILHYRELNTDGYRVFY
EIVDADQAIVVGLVLRDKQSVEQALIRYCLLHTD

Nucleotide

• Length: 345 bp

>T30796 NZ_AP014628:c6700426-6700082 [Pseudomonas sp. St29]
ATGCCAACACTGACCATCCGCTACACCGAAACCGCACAGCAAAGCATTGAAGACCAGATCGTGCATCTGGCCGACTATAG
CGACCTGAAGTCCGCTTTTGAGCGGCTGGATGCTGTGATTGACGCTCTTCAGGACAAGCTCTTGTCCGCCCCGTTAGGCT
ACCCAGTCAGCCCTCAAGCAAGCGACCTGGGCATCTTGCATTACCGTGAGCTCAACACCGATGGATACCGGGTGTTCTAC
GAAATCGTAGACGCGGATCAGGCGATCGTGGTCGGGCTGGTGCTGCGGGATAAGCAAAGCGTCGAGCAGGCACTTATTCG
TTACTGCCTGCTGCATACCGACTGA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9410.89 Da
• Isoelectric Point: 9.8184

>AT30796 WP_025131512.1 NZ_AP014628:c6700687-6700439 [Pseudomonas sp. St29]
MRVETVSFLKRYASDLPLEEPMVVTQNGVPVYVVESYVERKRRDEAVALVKLLAVSSREYAQGKHCSTDELKARLSKRFA
HK

Nucleotide

• Length: 249 bp

>AT30796 NZ_AP014628:c6700687-6700439 [Pseudomonas sp. St29]
ATGCGAGTTGAAACGGTCAGCTTTTTAAAACGATACGCATCCGACCTTCCTTTAGAGGAGCCAATGGTCGTTACGCAAAA
CGGTGTGCCTGTTTACGTTGTCGAGTCTTACGTTGAGCGTAAGCGCCGCGACGAGGCAGTGGCGTTGGTGAAATTGCTCG
CCGTCAGTTCGCGCGAGTACGCCCAAGGCAAGCATTGCTCGACCGATGAGCTGAAGGCCCGCTTAAGCAAGAGGTTTGCC
CACAAGTAA

Structures

Antitoxin

• AlphaFold DB: A0A7Y1CEW2