Detailed information of TA system TA030790

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/upstrm_HI1419-dnstrm_HI1420
• Location: 734175..734771
• Replicon: chromosome
• Accession: NZ_AP014628
• Organism: Pseudomonas sp. St29

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: PST29_RS03250
• Protein ID: WP_060842361.1
• Coordinates: 734469..734771 (-)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A2T6GFH6
• Locus tag: PST29_RS03245
• Protein ID: WP_060842360.1
• Coordinates: 734175..734465 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PST29_RS03225	730444..732576	-	2133	WP_060842359.1	TonB-dependent copper receptor	-
PST29_RS03230	732665..733075	-	411	WP_060837324.1	DUF2946 domain-containing protein	-
PST29_RS03235	733133..733612	-	480	WP_173655926.1	copper chaperone PCu(A)C	-
PST29_RS03240	733677..734075	-	399	WP_060837325.1	DUF2946 domain-containing protein	-
PST29_RS03245	734175..734465	-	291	WP_060842360.1	putative addiction module antidote protein	Antitoxin
PST29_RS03250	734469..734771	-	303	WP_060842361.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
PST29_RS03255	734945..735673	-	729	WP_060842362.1	cobalt-precorrin-6A reductase	-
PST29_RS03260	735670..736767	-	1098	WP_060837329.1	cobalt-precorrin-5B (C(1))-methyltransferase	-
PST29_RS03265	736861..738075	-	1215	WP_060842363.1	bifunctional cobalt-precorrin-7 (C(5))-methyltransferase/cobalt-precorrin-6B (C(15))-methyltransferase	-
PST29_RS03270	738207..739520	+	1314	WP_060842364.1	precorrin-3B synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (4-95)

Antitoxin

• Uncharacterized protein HI1420 (5-94)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11510.24 Da
• Isoelectric Point: 10.4937

>T30790 WP_060842361.1 NZ_AP014628:c734771-734469 [Pseudomonas sp. St29]
MYVFEQTLEFSRWLLHLKDPQGKTRVLARIRAAQLGRFGDCEALGGGVYEMRIHSGPGYRVYFSRRGTVIYLLLIGGDKN
TQRRDIQRARQLAHDLKSEG

Nucleotide

• Length: 303 bp

>T30790 NZ_AP014628:c734771-734469 [Pseudomonas sp. St29]
ATGTACGTCTTCGAGCAGACCCTGGAGTTCAGCCGCTGGCTCCTGCACCTGAAGGATCCGCAAGGCAAAACCCGTGTACT
GGCCAGGATCCGCGCCGCCCAACTGGGCCGATTCGGCGACTGCGAAGCACTGGGTGGAGGCGTCTACGAGATGCGCATCC
ATAGTGGTCCCGGCTACCGGGTGTACTTCTCGCGCCGGGGCACGGTGATCTACCTGTTGTTGATCGGCGGTGACAAGAAC
ACCCAGAGACGCGATATCCAACGCGCCAGGCAACTGGCCCACGACCTCAAGAGCGAGGGTTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10454.01 Da
• Isoelectric Point: 6.4594

>AT30790 WP_060842360.1 NZ_AP014628:c734465-734175 [Pseudomonas sp. St29]
MHTPVTRFDAAEYLKTPEDMVAYLDACFEEDTGDGKLIRAALNDIARARGMTQIARDTGLGRESLYKALGSQGNPEFATI
IKVMKALGLKLHVSPA

Nucleotide

• Length: 291 bp

>AT30790 NZ_AP014628:c734465-734175 [Pseudomonas sp. St29]
ATGCACACCCCAGTCACCCGCTTCGACGCGGCGGAGTACCTCAAGACCCCTGAAGACATGGTCGCCTACCTCGACGCCTG
CTTCGAGGAAGACACCGGCGACGGCAAGCTGATCCGCGCCGCCCTCAACGATATCGCCCGCGCCCGCGGCATGACCCAGA
TCGCCCGCGATACCGGCCTGGGCCGCGAGAGCCTGTACAAGGCCCTGGGCAGCCAGGGCAATCCCGAGTTCGCAACTATC
ATCAAGGTCATGAAAGCGCTCGGACTCAAGCTCCACGTCAGCCCGGCCTGA

Structures

Antitoxin

• AlphaFold DB: A0A2T6GFH6