Detailed information of TA system TA030781

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 285255..285868
• Replicon: chromosome
• Accession: NZ_AP014627
• Organism: Pseudomonas sp. Os17

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A2T6GEG0
• Locus tag: POS17_RS01315
• Protein ID: WP_060837021.1
• Coordinates: 285255..285437 (+)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: POS17_RS01320
• Protein ID: WP_173655881.1
• Coordinates: 285434..285868 (+)
• Length: 145 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
POS17_RS01300	281340..282266	+	927	WP_041115170.1	serine acetyltransferase	-
POS17_RS01305	282765..284726	+	1962	WP_162492852.1	choline transporter BetT	-
POS17_RS01310	284736..284993	-	258	WP_060837020.1	hypothetical protein	-
POS17_RS01315	285255..285437	+	183	WP_060837021.1	type II toxin-antitoxin system HicA family toxin	Toxin
POS17_RS01320	285434..285868	+	435	WP_173655881.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
POS17_RS01325	285871..286917	-	1047	WP_060837022.1	LacI family DNA-binding transcriptional regulator	-
POS17_RS01330	287123..289381	+	2259	WP_060837023.1	TonB-dependent receptor	-
POS17_RS01335	289484..290812	+	1329	WP_060837024.1	LLM class flavin-dependent oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (3-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (14-138)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6880.02 Da
• Isoelectric Point: 10.7495

>T30781 WP_060837021.1 NZ_AP014627:285255-285437 [Pseudomonas sp. Os17]
MRSREMIGKIEADGWYLIAVKGSHHQYKHPWKPGRVTFKHPDAELPRGTLNSILKQAGLK

Nucleotide

• Length: 183 bp

>T30781 NZ_AP014627:285255-285437 [Pseudomonas sp. Os17]
ATGCGCAGTCGGGAGATGATCGGCAAGATTGAGGCGGATGGTTGGTACCTGATTGCCGTGAAGGGCAGTCACCATCAGTA
CAAGCATCCCTGGAAACCAGGGCGCGTCACTTTCAAGCATCCGGATGCGGAACTGCCCAGGGGCACGCTCAACAGCATCT
TGAAACAGGCGGGCTTGAAATGA

Antitoxin

Protein

• Length: 145 a.a.
• Molecular weight: 15907.78 Da
• Isoelectric Point: 4.5688

>AT30781 WP_173655881.1 NZ_AP014627:285434-285868 [Pseudomonas sp. Os17]
MSPLLHEESNDMKFPVVLHKDADSEYGVIVPDVPGCYSAGHTVSLAFENVKEALSLHYAGLVADGEPLPQVHEIDEHLDN
PDYAGGVWGVVEFDVTPYFGKSVRFNATLPEHLLERIDQTVKRDQRYNSRSGFLAAAALRELSA

Nucleotide

• Length: 435 bp

>AT30781 NZ_AP014627:285434-285868 [Pseudomonas sp. Os17]
ATGAGCCCGTTACTGCATGAGGAGTCCAACGACATGAAATTCCCGGTGGTTCTGCACAAGGATGCCGACTCTGAATACGG
GGTGATCGTTCCGGATGTGCCGGGCTGTTACTCCGCTGGTCACACGGTGTCCCTGGCGTTCGAGAACGTGAAGGAGGCGT
TGTCCCTGCATTACGCAGGGCTGGTGGCCGATGGCGAGCCTCTGCCGCAGGTGCACGAGATCGATGAGCACCTGGACAAC
CCCGACTACGCCGGGGGTGTATGGGGCGTGGTGGAGTTCGACGTGACGCCGTATTTCGGCAAGTCGGTGCGCTTCAATGC
CACCCTGCCGGAACACCTGCTGGAGCGCATCGATCAGACGGTCAAGCGTGACCAGCGCTACAACTCGCGTTCCGGGTTCC
TGGCCGCCGCGGCGCTGCGCGAACTGTCGGCCTGA

Structures

Toxin

• AlphaFold DB: A0A2T6GEG0