Detailed information of TA system TA030771

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 125189..125802
• Replicon: chromosome
• Accession: NZ_AP014623
• Organism: Pseudomonas chlororaphis subsp. aurantiaca strain StFRB508

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: PCAU_RS00545
• Protein ID: WP_096372784.1
• Coordinates: 125189..125371 (+)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A554PT90
• Locus tag: PCAU_RS00550
• Protein ID: WP_009046450.1
• Coordinates: 125401..125802 (+)
• Length: 134 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PCAU_RS00515	120955..121212	-	258	WP_028683851.1	hypothetical protein	-
PCAU_RS00520	121265..121753	-	489	WP_096372781.1	DoxX family protein	-
PCAU_RS00525	121827..122723	-	897	WP_096372782.1	SDR family oxidoreductase	-
PCAU_RS00530	122752..123261	-	510	WP_081362986.1	DUF1097 domain-containing protein	-
PCAU_RS00535	123295..124035	-	741	WP_096372783.1	SDR family oxidoreductase	-
PCAU_RS00540	124174..125064	+	891	WP_081362984.1	LysR family transcriptional regulator	-
PCAU_RS00545	125189..125371	+	183	WP_096372784.1	type II toxin-antitoxin system HicA family toxin	Toxin
PCAU_RS00550	125401..125802	+	402	WP_009046450.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
PCAU_RS00555	125902..126948	-	1047	WP_081362983.1	LacI family DNA-binding transcriptional regulator	-
PCAU_RS00560	127156..129414	+	2259	WP_096372785.1	TonB-dependent receptor	-
PCAU_RS00565	129461..130786	+	1326	WP_096372786.1	LLM class flavin-dependent oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (3-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-127)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6902.07 Da
• Isoelectric Point: 10.4588

>T30771 WP_096372784.1 NZ_AP014623:125189-125371 [Pseudomonas chlororaphis subsp. aurantiaca]
MRSRELIRMIEEDGWYLIAVKGSHHPYKHLHKPGRVTIPHPDADLPRGTIHSILKQAGLK

Nucleotide

• Length: 183 bp

>T30771 NZ_AP014623:125189-125371 [Pseudomonas chlororaphis subsp. aurantiaca]
ATGCGCAGTCGGGAGCTGATCAGGATGATCGAGGAGGACGGGTGGTATCTGATCGCGGTCAAAGGCAGTCATCACCCGTA
CAAACATCTGCACAAGCCGGGGCGGGTCACTATCCCTCACCCGGATGCGGATCTGCCCAGGGGCACGATCCACAGCATCT
TGAAACAGGCGGGCCTGAAATGA

Antitoxin

Protein

• Length: 134 a.a.
• Molecular weight: 14596.44 Da
• Isoelectric Point: 4.9133

>AT30771 WP_009046450.1 NZ_AP014623:125401-125802 [Pseudomonas chlororaphis subsp. aurantiaca]
MKFPVVLHKDADSGYGVIVPDVPGCFSAGHTAAQAFENVKEALSLHYEGLVADGEPLPQVHEVDVHIDNPDYAGGVWGVI
EFDITPYFGKAVRFNATLPEQLLERIDQTVKRDQRYRSRSGFLAAAALRELSA

Nucleotide

• Length: 402 bp

>AT30771 NZ_AP014623:125401-125802 [Pseudomonas chlororaphis subsp. aurantiaca]
ATGAAATTCCCGGTCGTGCTGCACAAGGATGCCGACTCCGGATATGGAGTGATCGTCCCCGATGTGCCGGGCTGTTTTTC
CGCCGGTCATACCGCTGCGCAAGCCTTCGAGAATGTGAAAGAAGCCCTGTCGCTGCATTACGAAGGCCTGGTCGCCGATG
GTGAGCCGTTGCCCCAGGTGCATGAAGTCGACGTGCATATCGACAATCCCGATTACGCCGGTGGCGTGTGGGGGGTAATC
GAGTTCGACATCACGCCTTACTTCGGCAAGGCGGTGCGTTTCAATGCGACATTGCCGGAGCAACTGTTGGAGCGCATCGA
CCAGACGGTGAAGCGCGACCAGCGCTACCGTTCGCGCTCGGGTTTTCTCGCGGCAGCGGCATTGCGCGAGTTGTCGGCGT
AG

Structures

Antitoxin

• AlphaFold DB: A0A554PT90