Detailed information of TA system TA030760

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpA-brnA/BrnT_toxin-BrnA
• Location: 2224977..2225508
• Replicon: chromosome
• Accession: NZ_AP014582
• Organism: Bordetella bronchiseptica strain S798

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: K0MH61
• Locus tag: BBS798_RS10385
• Protein ID: WP_003812626.1
• Coordinates: 2225242..2225508 (-)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: -
• Locus tag: BBS798_RS24445
• Protein ID: WP_003812628.1
• Coordinates: 2224977..2225255 (-)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BBS798_RS10360	2220407..2221501	+	1095	WP_003812637.1	TRAP transporter substrate-binding protein	-
BBS798_RS10365	2221713..2222342	+	630	WP_033451894.1	TRAP transporter small permease subunit	-
BBS798_RS10370	2222345..2224012	+	1668	WP_003812634.1	TRAP transporter large permease subunit	-
BBS798_RS10375	2224027..2224683	+	657	WP_003812630.1	phosphoribosylanthranilate isomerase	-
BBS798_RS24445	2224977..2225255	-	279	WP_003812628.1	BrnA antitoxin family protein	Antitoxin
BBS798_RS10385	2225242..2225508	-	267	WP_003812626.1	BrnT family toxin	Toxin
BBS798_RS10390	2225739..2227280	-	1542	WP_003812624.1	M81 family metallopeptidase	-
BBS798_RS10395	2227342..2228220	-	879	WP_033450228.1	tripartite tricarboxylate transporter substrate binding protein	-
BBS798_RS10400	2228617..2229249	+	633	WP_003812619.1	PAS domain-containing protein	-
BBS798_RS10405	2229360..2230070	+	711	WP_003812617.1	aquaporin Z	-
BBS798_RS10410	2230109..2230381	-	273	WP_003812615.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-81)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (12-86)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10037.47 Da
• Isoelectric Point: 7.9923

>T30760 WP_003812626.1 NZ_AP014582:c2225508-2225242 [Bordetella bronchiseptica]
MDITYDPAKNDKNIADRGLSFELVCGFEWASALVVEDTRQVYAETRYQALGKIDGRLHMVVFTVRGESLRIISLRKANAR
EVARYEKA

Nucleotide

• Length: 267 bp

>T30760 NZ_AP014582:c2225508-2225242 [Bordetella bronchiseptica]
ATGGATATCACCTACGACCCCGCCAAGAACGACAAGAACATTGCCGATCGCGGCCTGTCGTTCGAGTTGGTGTGTGGCTT
CGAGTGGGCCAGCGCGCTGGTGGTTGAGGATACCCGCCAGGTCTACGCCGAAACGCGCTATCAGGCGCTGGGCAAAATCG
ATGGCCGGCTCCATATGGTGGTATTCACGGTGCGCGGCGAGTCGCTGCGCATCATCAGCCTGCGTAAGGCAAATGCTCGT
GAGGTAGCTCGTTATGAAAAAGCCTGA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10426.92 Da
• Isoelectric Point: 10.5548

>AT30760 WP_003812628.1 NZ_AP014582:c2225255-2224977 [Bordetella bronchiseptica]
MKKPDPEKIDRDNPEWTDAEFRRAKPAAEVLPESVHAMLGIRRRGPQKTPTKQIVTIRLSPDVVEAFKASGTGWQTRVDA
ALRDWLKTHQPG

Nucleotide

• Length: 279 bp

>AT30760 NZ_AP014582:c2225255-2224977 [Bordetella bronchiseptica]
ATGAAAAAGCCTGACCCCGAAAAGATTGACCGCGACAACCCCGAATGGACGGACGCGGAATTTCGGCGGGCCAAGCCGGC
CGCCGAAGTACTGCCCGAGTCGGTGCACGCGATGCTCGGCATTCGTCGCCGTGGGCCTCAAAAGACACCCACCAAACAGA
TCGTCACCATCCGGCTATCGCCTGACGTGGTAGAAGCCTTCAAGGCCTCCGGCACCGGTTGGCAGACTCGCGTGGATGCA
GCCCTGCGGGATTGGCTGAAGACGCACCAGCCTGGCTAG

Structures

Toxin

• AlphaFold DB: K0MH61