Detailed information of TA system TA030753

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 3188976..3189663
• Replicon: chromosome
• Accession: NZ_AP014573
• Organism: Mycobacterium tuberculosis str. Kurono

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P65044
• Locus tag: KURONO_RS15200
• Protein ID: WP_003414624.1
• Coordinates: 3189220..3189663 (+)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TFH0
• Locus tag: KURONO_RS15195
• Protein ID: WP_003414620.1
• Coordinates: 3188976..3189233 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KURONO_RS15175	3184296..3185150	-	855	WP_003899516.1	GNAT family N-acetyltransferase	-
KURONO_RS15180	3185206..3186369	-	1164	WP_003899517.1	flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase	-
KURONO_RS15185	3186386..3187600	-	1215	WP_003899518.1	zinc metalloprotease Rip	-
KURONO_RS15190	3187608..3188849	-	1242	WP_003414613.1	1-deoxy-D-xylulose-5-phosphate reductoisomerase	-
KURONO_RS15195	3188976..3189233	+	258	WP_003414620.1	ribbon-helix-helix domain-containing protein	Antitoxin
KURONO_RS15200	3189220..3189663	+	444	WP_003414624.1	type II toxin-antitoxin system VapC family toxin	Toxin
KURONO_RS15205	3189743..3190405	+	663	WP_003414630.1	cell surface glycolipoprotein Mpt83	-
KURONO_RS15210	3190501..3190692	+	192	WP_003414632.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
KURONO_RS15215	3191024..3192772	+	1749	WP_003912869.1	cytochrome c biogenesis protein DipZ	-
KURONO_RS15220	3192868..3193449	+	582	WP_003414644.1	fasciclin domain-containing protein	-
KURONO_RS15225	3193549..3193815	+	267	WP_015456449.1	DUF2631 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain toxin (1-142)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-38)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16595.72 Da
• Isoelectric Point: 6.4890

>T30753 WP_003414624.1 NZ_AP014573:3189220-3189663 [Mycobacterium tuberculosis str. Kurono]
MLCVDVNVLVYAHRADLREHADYRGLLERLANDDEPLGLPDSVLAGFIRVVTNRRVFTEPTSPQDAWQAVDALLAAPAAM
RLRPGERHWMAFRQLASDVDANGNDIADAHLAAYALENNATWLSADRGFARFRRLRWRHPLDGQTHL

Nucleotide

• Length: 444 bp

>T30753 NZ_AP014573:3189220-3189663 [Mycobacterium tuberculosis str. Kurono]
ATGCTGTGCGTTGATGTCAACGTGCTCGTTTACGCGCATCGGGCAGACCTACGGGAGCACGCGGACTATCGGGGTTTGCT
TGAGCGGCTGGCCAACGATGACGAGCCGCTGGGTCTACCAGATAGCGTGCTCGCCGGCTTCATCCGGGTGGTTACCAACC
GCCGCGTCTTCACCGAGCCGACGAGCCCACAGGACGCATGGCAGGCAGTCGACGCCCTACTCGCGGCACCCGCAGCCATG
CGACTTCGGCCTGGCGAGCGCCACTGGATGGCCTTTCGGCAGTTAGCGTCCGATGTTGATGCGAACGGCAACGACATTGC
GGACGCGCACCTGGCCGCCTACGCGCTAGAGAACAACGCAACCTGGTTGAGCGCCGACCGCGGCTTTGCCCGTTTCCGTC
GACTGCGCTGGCGTCATCCGTTGGACGGTCAGACCCATCTATAA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9013.22 Da
• Isoelectric Point: 10.8344

>AT30753 WP_003414620.1 NZ_AP014573:3188976-3189233 [Mycobacterium tuberculosis str. Kurono]
VRTTIRIDDELYREVKAKAARSGRTVAAVLEDAVRRGLNPPKPQAAGRYRVQPSGKGGLRPGVDLSSNAALAEAMNDGVS
VDAVR

Nucleotide

• Length: 258 bp

>AT30753 NZ_AP014573:3188976-3189233 [Mycobacterium tuberculosis str. Kurono]
GTGCGCACGACGATCCGTATCGATGACGAGCTGTACCGCGAGGTGAAAGCAAAGGCCGCTCGTTCCGGGCGTACCGTGGC
CGCGGTTCTTGAAGATGCGGTGCGGCGTGGTCTCAACCCGCCTAAGCCGCAGGCCGCCGGCCGTTATCGAGTCCAGCCGT
CGGGTAAGGGCGGCCTGCGGCCCGGTGTCGATCTATCGTCCAACGCCGCACTTGCCGAAGCGATGAACGACGGCGTGTCG
GTCGATGCTGTGCGTTGA

Structures

Toxin

• PDB: 7VWO

Antitoxin

• PDB: 7VWO
• AlphaFold DB: G0TFH0