Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN_Sll0205-like-Phd
Location 3142458..3143062 Replicon chromosome
Accession NZ_AP014573
Organism Mycobacterium tuberculosis str. Kurono

Toxin (Protein)


Gene name vapC Uniprot ID P71623
Locus tag KURONO_RS14970 Protein ID WP_003414492.1
Coordinates 3142458..3142850 (-) Length 131 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P71622
Locus tag KURONO_RS14975 Protein ID WP_003912003.1
Coordinates 3142847..3143062 (-) Length 72 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KURONO_RS14940 3137608..3138396 - 789 WP_003917092.1 CRISPR-associated endoribonuclease Cas6 -
KURONO_RS14945 3138730..3139275 - 546 WP_003913758.1 DUF1802 family protein -
KURONO_RS14950 3139547..3140431 - 885 WP_003414409.1 nucleotidyl transferase AbiEii/AbiGii toxin family protein -
KURONO_RS14955 3140434..3141321 - 888 WP_003414414.1 type IV toxin-antitoxin system AbiEi family antitoxin -
KURONO_RS14960 3141626..3142171 - 546 WP_003899500.1 DUF1802 family protein -
KURONO_RS14965 3142168..3142437 - 270 WP_003414489.1 DUF2277 family protein -
KURONO_RS14970 3142458..3142850 - 393 WP_003414492.1 type II toxin-antitoxin system VapC family toxin Toxin
KURONO_RS14975 3142847..3143062 - 216 WP_003912003.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
KURONO_RS14980 3143109..3143858 + 750 WP_003414497.1 enoyl-CoA hydratase -
KURONO_RS14985 3143937..3145019 - 1083 WP_003414499.1 ABC transporter ATP-binding protein -
KURONO_RS14990 3145012..3146322 - 1311 WP_003414501.1 ABC transporter substrate-binding protein -
KURONO_RS14995 3146325..3147152 - 828 WP_003414504.1 carbohydrate ABC transporter permease -
KURONO_RS15000 3147149..3148060 - 912 WP_003414505.1 sugar ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

VapC-like Sll0205 protein, PIN domain

(5-130)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(2-46)


Sequences

Toxin        


Download         Length: 131 a.a.        Molecular weight: 14610.74 Da        Isoelectric Point: 6.7594

>T30751 WP_003414492.1 NZ_AP014573:c3142850-3142458 [Mycobacterium tuberculosis str. Kurono]
MTTVLLDSHVAYWWSAEPQRLSMAASQAIEHADELAVAAISWFELAWLAEQERIQLAIPVLSWLQQLAEHVRTVGITPSV
AATAVALPSSFPGDPADRLIYATAIEHGWRLVTKDRRLRSHRHPRPVTVW

Download         Length: 393 bp

>T30751 NZ_AP014573:c3142850-3142458 [Mycobacterium tuberculosis str. Kurono]
ATGACGACGGTGCTGCTCGACTCGCATGTGGCCTACTGGTGGTCGGCCGAGCCGCAGCGTCTCAGCATGGCGGCGAGCCA
GGCCATCGAACACGCCGACGAGCTCGCCGTCGCCGCGATTTCGTGGTTCGAGCTGGCTTGGCTTGCCGAACAGGAACGCA
TCCAACTGGCGATTCCGGTGCTGTCCTGGCTTCAGCAGCTGGCCGAGCACGTTCGCACCGTCGGTATCACGCCCTCGGTC
GCCGCCACGGCGGTGGCGCTGCCCTCGTCGTTCCCCGGCGATCCGGCCGACCGGTTGATCTACGCCACCGCGATCGAACA
CGGCTGGCGGCTGGTGACCAAGGACCGGCGGCTACGCAGTCATCGGCACCCACGACCGGTCACCGTCTGGTAG

Antitoxin


Download         Length: 72 a.a.        Molecular weight: 7483.50 Da        Isoelectric Point: 4.9732

>AT30751 WP_003912003.1 NZ_AP014573:c3143062-3142847 [Mycobacterium tuberculosis str. Kurono]
MTATEVKAKILSLLDEVAQGEEIEITKHGRTVARLVAATGPHALKGRFSGVAMAAADDDELFTTGVSWNVS

Download         Length: 216 bp

>AT30751 NZ_AP014573:c3143062-3142847 [Mycobacterium tuberculosis str. Kurono]
ATGACCGCTACGGAGGTGAAGGCGAAGATCCTCTCCTTGCTTGATGAAGTGGCCCAGGGCGAGGAGATCGAGATCACCAA
ACACGGCCGCACCGTGGCCCGGCTGGTGGCAGCGACGGGGCCGCACGCGCTGAAGGGTCGATTCTCGGGTGTGGCGATGG
CGGCCGCGGATGACGACGAACTCTTCACCACCGGGGTTTCGTGGAACGTTTCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BWM8

Antitoxin

Source ID Structure
AlphaFold DB P71622

References