Detailed information of TA system TA030749

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 3114566..3115136
• Replicon: chromosome
• Accession: NZ_AP014573
• Organism: Mycobacterium tuberculosis str. Kurono

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: P71650
• Locus tag: KURONO_RS14810
• Protein ID: WP_003414166.1
• Coordinates: 3114566..3114922 (-)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: P0CL61
• Locus tag: KURONO_RS14815
• Protein ID: WP_003901465.1
• Coordinates: 3114906..3115136 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KURONO_RS14790	3110018..3111706	-	1689	WP_003414155.1	alpha/beta hydrolase family protein	-
KURONO_RS14795	3111710..3112036	-	327	WP_003414157.1	hypothetical protein	-
KURONO_RS14800	3112209..3112796	+	588	WP_003914429.1	DUF3558 family protein	-
KURONO_RS14805	3112815..3114464	+	1650	WP_003899485.1	CocE/NonD family hydrolase	-
KURONO_RS14810	3114566..3114922	-	357	WP_003414166.1	type II toxin-antitoxin system toxin endoribonuclease MazF9	Toxin
KURONO_RS14815	3114906..3115136	-	231	WP_003901465.1	antitoxin MazE	Antitoxin
KURONO_RS14820	3115179..3116222	-	1044	WP_003414172.1	DUF2293 domain-containing protein	-
KURONO_RS22270	3116413..3116688	+	276	WP_003911993.1	DUF1778 domain-containing protein	-
KURONO_RS22445	3116864..3117118	-	255	WP_003917684.1	hypothetical protein	-
KURONO_RS14835	3117266..3117670	+	405	WP_009938577.1	hypothetical protein	-
KURONO_RS14840	3117667..3117858	+	192	WP_003414184.1	hypothetical protein	-
KURONO_RS14845	3117922..3119211	+	1290	Protein_2910	transposase family protein	-
KURONO_RS14850	3119445..3119702	+	258	WP_003899489.1	hypothetical protein	-
KURONO_RS14855	3119807..3120118	+	312	WP_003414190.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3104601..3128125	23524

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-113)

Antitoxin

• Ribbon-helix-helix protein, CopG (3-29)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 12858.73 Da
• Isoelectric Point: 9.1962

>T30749 WP_003414166.1 NZ_AP014573:c3114922-3114566 [Mycobacterium tuberculosis str. Kurono]
VMRRGEIWQVDLDPARGSEANNQRPAVVVSNDRANATATRLGRGVITVVPVTSNIAKVYPFQVLLSATTTGLQVDCKAQA
EQIRSIATERLLRPIGRVSAAELAQLDEALKLHLDLWS

Nucleotide

• Length: 357 bp

>T30749 NZ_AP014573:c3114922-3114566 [Mycobacterium tuberculosis str. Kurono]
GTGATGCGCCGCGGTGAGATTTGGCAGGTCGATCTCGACCCCGCTCGAGGTAGCGAAGCGAACAACCAGCGCCCCGCCGT
CGTCGTCAGCAACGACCGGGCCAACGCGACCGCCACGCGTCTTGGGCGCGGCGTCATCACCGTCGTGCCGGTGACGAGCA
ACATCGCCAAGGTCTATCCGTTTCAGGTGTTGTTGTCGGCCACCACTACTGGTCTCCAGGTCGACTGCAAGGCGCAGGCC
GAGCAAATCAGATCGATTGCTACCGAGCGGTTGCTCCGGCCAATCGGCCGAGTTTCAGCCGCCGAACTTGCCCAGCTCGA
TGAGGCTTTGAAACTGCATCTCGACTTATGGTCGTAG

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8231.01 Da
• Isoelectric Point: 4.1809

>AT30749 WP_003901465.1 NZ_AP014573:c3115136-3114906 [Mycobacterium tuberculosis str. Kurono]
VKLSVSLSDDDVAILDAYVKRAGLPSRSAGLQHAIRVLRYPTLEDDYANAWQEWSAAGDTDAWEQTVGDGVGDAPR

Nucleotide

• Length: 231 bp

>AT30749 NZ_AP014573:c3115136-3114906 [Mycobacterium tuberculosis str. Kurono]
GTGAAGTTGAGCGTGAGCCTGTCCGATGACGACGTCGCGATCCTGGACGCATATGTGAAACGAGCGGGATTACCATCCCG
CTCCGCCGGCCTGCAGCATGCGATCCGCGTGCTTCGCTACCCCACGCTCGAAGATGACTATGCCAACGCATGGCAAGAAT
GGTCGGCAGCCGGCGACACGGACGCGTGGGAGCAGACCGTCGGCGACGGAGTCGGTGATGCGCCGCGGTGA

Structures

Toxin

• PDB: 6L2A
• PDB: 5HJZ
• PDB: 6KYT
• PDB: 6L29
• PDB: 7DU5
• PDB: 6KYS

Antitoxin

• AlphaFold DB: A0A7U4FBW3