Detailed information of TA system TA030748

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 3074982..3075669
• Replicon: chromosome
• Accession: NZ_AP014573
• Organism: Mycobacterium tuberculosis str. Kurono

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TF65
• Locus tag: KURONO_RS14600
• Protein ID: WP_003414064.1
• Coordinates: 3075274..3075669 (-)
• Length: 132 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TF64
• Locus tag: KURONO_RS14595
• Protein ID: WP_003414061.1
• Coordinates: 3074982..3075248 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KURONO_RS14570	3070621..3071523	-	903	WP_003900564.1	4-hydroxy-tetrahydrodipicolinate synthase	-
KURONO_RS14575	3071592..3072344	-	753	WP_003899465.1	FAD-dependent thymidylate synthase	-
KURONO_RS14580	3072588..3072863	-	276	WP_003414055.1	type I restriction endonuclease subunit S	-
KURONO_RS14585	3072860..3074482	-	1623	WP_003906897.1	type I restriction-modification system subunit M	-
KURONO_RS14590	3074569..3074985	-	417	WP_003414059.1	type II toxin-antitoxin system toxin ribonuclease C21	-
KURONO_RS14595	3074982..3075248	-	267	WP_003414061.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
KURONO_RS14600	3075274..3075669	-	396	WP_003414064.1	type II toxin-antitoxin system VapC family toxin	Toxin
KURONO_RS14605	3075666..3075935	-	270	WP_003414066.1	type II toxin-antitoxin system VapB family antitoxin	-
KURONO_RS14610	3075945..3077039	-	1095	WP_003414068.1	restriction endonuclease subunit S	-
KURONO_RS14615	3077036..3077455	-	420	WP_003414070.1	winged helix-turn-helix domain-containing protein	-
KURONO_RS14620	3077529..3078008	-	480	WP_003414073.1	dihydrofolate reductase	-
KURONO_RS14625	3078079..3078879	-	801	WP_003911953.1	thymidylate synthase	-
KURONO_RS14630	3079035..3079772	+	738	WP_003414079.1	dienelactone hydrolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-125)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (16-56)

Sequences

Toxin

Protein

• Length: 132 a.a.
• Molecular weight: 14340.04 Da
• Isoelectric Point: 4.7145

>T30748 WP_003414064.1 NZ_AP014573:c3075669-3075274 [Mycobacterium tuberculosis str. Kurono]
VIVDTSAIVAIVSGESGAQVLKEALERSPNSRMSAPNYVELCAIMQRRDRPEISRLVDRLLDDYGIQVEAVDADQARVAA
QAYRDYGRGSGHPARLNLGDTYSYALAQVTGEPLLFRGDDFTHTDIRPACT

Nucleotide

• Length: 396 bp

>T30748 NZ_AP014573:c3075669-3075274 [Mycobacterium tuberculosis str. Kurono]
GTGATCGTCGATACGTCGGCCATCGTCGCCATCGTGAGCGGGGAATCGGGCGCGCAGGTGCTCAAGGAGGCGCTGGAGCG
GTCACCGAACTCCCGAATGTCCGCGCCCAACTACGTCGAACTGTGCGCGATCATGCAGCGGCGGGACCGGCCGGAGATCT
CTCGATTGGTGGACCGTTTGCTGGACGACTACGGAATCCAGGTCGAAGCCGTCGACGCCGACCAAGCCCGCGTGGCCGCG
CAGGCGTATCGCGACTACGGCCGCGGCAGCGGCCATCCGGCCCGTCTCAACCTCGGCGACACCTACAGCTACGCCCTGGC
CCAGGTCACCGGGGAGCCGCTGCTGTTTCGTGGCGATGACTTCACGCATACCGATATCCGGCCCGCGTGCACCTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9493.77 Da
• Isoelectric Point: 7.2783

>AT30748 WP_003414061.1 NZ_AP014573:c3075248-3074982 [Mycobacterium tuberculosis str. Kurono]
MHRGYALVVCSPGVTRTMIDIDDDLLARAAKELGTTTKKDTVHAALRAALRASAARSLMNRMAENATGTQDEALVNAMWR
DGHPENTA

Nucleotide

• Length: 267 bp

>AT30748 NZ_AP014573:c3075248-3074982 [Mycobacterium tuberculosis str. Kurono]
ATGCATCGCGGATATGCATTAGTAGTATGCTCTCCGGGTGTGACGCGCACCATGATCGACATCGATGACGACCTGCTCGC
TCGGGCCGCCAAGGAACTGGGTACCACGACAAAGAAAGACACCGTGCATGCCGCGCTGCGGGCCGCGTTGCGAGCCAGTG
CCGCGCGCTCGCTCATGAACCGGATGGCCGAGAACGCCACCGGAACCCAGGACGAAGCTCTGGTCAACGCAATGTGGCGA
GACGGCCACCCAGAGAACACGGCATGA

Structures

Toxin

• AlphaFold DB: G0TF65

Antitoxin

• AlphaFold DB: G0TF64