Detailed information of TA system TA030741

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 2858668..2859308
• Replicon: chromosome
• Accession: NZ_AP014573
• Organism: Mycobacterium tuberculosis str. Kurono

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ08
• Locus tag: KURONO_RS13415
• Protein ID: WP_003412970.1
• Coordinates: 2858668..2859087 (-)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TQ09
• Locus tag: KURONO_RS13420
• Protein ID: WP_003412975.1
• Coordinates: 2859084..2859308 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KURONO_RS13390	2854253..2854975	-	723	WP_003412957.1	DUF1906 domain-containing protein	-
KURONO_RS13395	2855492..2855719	+	228	WP_003412960.1	type II toxin-antitoxin system VapB family antitoxin	-
KURONO_RS13400	2855716..2856117	+	402	WP_003412963.1	PIN domain-containing protein	-
KURONO_RS13405	2856152..2857072	-	921	WP_003412965.1	restriction endonuclease	-
KURONO_RS22535	2857413..2857658	-	246	WP_071854223.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
KURONO_RS13410	2857717..2858667	+	951	WP_003911916.1	Lsr2 family protein	-
KURONO_RS13415	2858668..2859087	-	420	WP_003412970.1	type II toxin-antitoxin system VapC family toxin	Toxin
KURONO_RS13420	2859084..2859308	-	225	WP_003412975.1	antitoxin VapB39	Antitoxin
KURONO_RS13425	2859339..2862182	-	2844	WP_003899363.1	aminotransferase class I/II-fold pyridoxal phosphate-dependent enzyme	-
KURONO_RS13430	2862254..2862655	-	402	WP_003412981.1	hypothetical protein	-
KURONO_RS13435	2862655..2863125	-	471	WP_003899364.1	transcription antitermination factor NusB	-
KURONO_RS13440	2863128..2863691	-	564	WP_003412989.1	elongation factor P	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-128)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 14732.70 Da
• Isoelectric Point: 6.7519

>T30741 WP_003412970.1 NZ_AP014573:c2859087-2858668 [Mycobacterium tuberculosis str. Kurono]
VTALLDVNVLIALGWPNHVHHAAAQRWFTQFSSNGWATTPITEAGYVRISSNRSVMQVSTTPAIAIAQLAAMTSLAGHTF
WPDDVPLIVGSAGDRDAVSNHRRVTDCHLIALAARYGGRLVTFDAALADSASAGLVEVL

Nucleotide

• Length: 420 bp

>T30741 NZ_AP014573:c2859087-2858668 [Mycobacterium tuberculosis str. Kurono]
GTGACGGCACTGCTCGATGTCAATGTGCTGATCGCGCTGGGCTGGCCGAATCACGTTCACCATGCGGCCGCGCAGCGATG
GTTCACGCAGTTCTCCTCGAATGGGTGGGCCACCACGCCGATCACCGAGGCAGGGTATGTCCGAATTTCAAGCAATCGCA
GTGTGATGCAGGTGTCGACCACGCCGGCTATCGCGATCGCTCAGTTGGCGGCGATGACTTCTCTTGCCGGGCACACGTTT
TGGCCTGACGATGTGCCACTGATCGTTGGGAGCGCCGGCGATCGCGATGCGGTGTCCAACCACCGTCGGGTCACCGACTG
CCATCTCATCGCCTTGGCCGCGCGCTACGGGGGCCGGTTGGTCACATTCGATGCCGCACTGGCCGATTCAGCATCCGCAG
GCCTCGTCGAGGTGTTGTAG

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 7993.94 Da
• Isoelectric Point: 4.0057

>AT30741 WP_003412975.1 NZ_AP014573:c2859308-2859084 [Mycobacterium tuberculosis str. Kurono]
MRTTLQIDDDVLEDARSIARSEGKSVGAVISELARRSLRPVGIVEVDGFPVFDVPPDAPTVTSEDVVRALEDDV

Nucleotide

• Length: 225 bp

>AT30741 NZ_AP014573:c2859308-2859084 [Mycobacterium tuberculosis str. Kurono]
ATGCGCACCACGTTGCAGATTGATGATGATGTTCTAGAAGATGCTCGTAGCATCGCGCGGTCGGAGGGCAAGTCAGTCGG
CGCGGTAATTTCTGAGTTGGCGCGTAGGTCGCTCCGTCCGGTTGGGATTGTCGAGGTTGACGGATTTCCGGTTTTTGATG
TTCCGCCGGATGCGCCGACGGTGACTTCCGAGGATGTCGTCCGCGCGCTCGAGGACGACGTGTGA

Structures

Toxin

• AlphaFold DB: G0TQ08

Antitoxin

• AlphaFold DB: G0TQ09