Detailed information of TA system TA030735

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 2263483..2264109
• Replicon: chromosome
• Accession: NZ_AP014573
• Organism: Mycobacterium tuberculosis str. Kurono

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P64926
• Locus tag: KURONO_RS10600
• Protein ID: WP_003410075.1
• Coordinates: 2263711..2264109 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A806JLL8
• Locus tag: KURONO_RS10595
• Protein ID: WP_003911750.1
• Coordinates: 2263483..2263710 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KURONO_RS10585	2261522..2261866	-	345	WP_003410065.1	ferredoxin family protein	-
KURONO_RS10590	2262055..2263224	-	1170	WP_003899126.1	ATP-binding protein	-
KURONO_RS10595	2263483..2263710	+	228	WP_003911750.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
KURONO_RS10600	2263711..2264109	+	399	WP_003410075.1	PIN domain nuclease	Toxin
KURONO_RS10605	2264292..2264723	-	432	WP_003410078.1	MarR family winged helix-turn-helix transcriptional regulator	-
KURONO_RS10610	2264824..2265258	+	435	WP_003914358.1	DUF1398 domain-containing protein	-
KURONO_RS21380	2265695..2265874	-	180	Protein_2085	hypothetical protein	-
KURONO_RS10615	2265935..2266582	+	648	WP_071854215.1	transposase	-
KURONO_RS10620	2266536..2267126	+	591	WP_003899131.1	IS110 family transposase	-
KURONO_RS10625	2267254..2268510	-	1257	WP_003410095.1	HNH endonuclease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-120)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (2-43)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14731.02 Da
• Isoelectric Point: 6.7067

>T30735 WP_003410075.1 NZ_AP014573:2263711-2264109 [Mycobacterium tuberculosis str. Kurono]
MIVDTSVWIAYLSTSESLASRWLADRIAADSTVIVPEVVMMELLIGKTDEDTAALRRRLLQRFAIEPLAPVRDAEDAAAI
HRRCRRGGDTVRSLIDCQVAAMALRIGVAVAHRDRDYEAIRTHCGLRTEPLF

Nucleotide

• Length: 399 bp

>T30735 NZ_AP014573:2263711-2264109 [Mycobacterium tuberculosis str. Kurono]
ATGATCGTCGACACCTCGGTCTGGATCGCATATCTCTCCACGTCAGAGTCGTTGGCCAGTCGCTGGCTAGCCGATCGCAT
TGCCGCTGACTCGACGGTGATCGTGCCCGAGGTGGTGATGATGGAGCTGCTGATCGGTAAGACCGATGAGGACACCGCCG
CACTGCGCCGACGGCTCCTGCAGCGATTCGCTATCGAACCGCTGGCCCCGGTCCGCGACGCGGAAGATGCCGCCGCCATT
CACCGGCGCTGTCGTCGCGGCGGCGACACCGTACGCAGCCTGATCGATTGCCAGGTGGCCGCGATGGCGTTGCGGATCGG
GGTCGCCGTGGCGCATCGTGATCGCGACTACGAGGCGATCCGCACACATTGCGGACTACGCACCGAGCCGTTGTTCTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8346.16 Da
• Isoelectric Point: 4.1621

>AT30735 WP_003911750.1 NZ_AP014573:2263483-2263710 [Mycobacterium tuberculosis str. Kurono]
VSRTNIEIDDELVAAAQRMYRLDSKRSAVDLALRRLVGEPLGRDEALALQGSGFDFSNDEIESFSDTDRKLADES

Nucleotide

• Length: 228 bp

>AT30735 NZ_AP014573:2263483-2263710 [Mycobacterium tuberculosis str. Kurono]
GTGTCACGGACCAACATCGAGATCGACGACGAACTCGTGGCCGCCGCACAGCGGATGTACCGACTCGATTCCAAGCGAAG
TGCCGTCGACCTCGCGCTGCGCCGGCTCGTGGGTGAACCGTTGGGCCGCGATGAGGCTTTGGCGCTGCAGGGCAGCGGTT
TCGACTTCAGCAACGATGAGATCGAATCGTTCTCGGATACGGACCGCAAGCTCGCCGACGAGTCGTAG

Structures

Toxin

• PDB: 4CHG

Antitoxin

• AlphaFold DB: A0A806JLL8