Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mbcAT/RES-TIGR02293
Location 2238177..2239075 Replicon chromosome
Accession NZ_AP014573
Organism Mycobacterium tuberculosis str. Kurono

Toxin (Protein)


Gene name mbcT Uniprot ID P64908
Locus tag KURONO_RS10475 Protein ID WP_003410001.1
Coordinates 2238177..2238737 (-) Length 187 a.a.

Antitoxin (Protein)


Gene name mbcA Uniprot ID P64910
Locus tag KURONO_RS10480 Protein ID WP_003410003.1
Coordinates 2238734..2239075 (-) Length 114 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KURONO_RS10445 2233346..2233999 - 654 WP_003409976.1 cutinase Cfp21 -
KURONO_RS10450 2234114..2234344 + 231 WP_003409980.1 DUF167 domain-containing protein -
KURONO_RS10455 2234429..2235340 - 912 WP_003409986.1 ArgP/LysG family DNA-binding transcriptional regulator -
KURONO_RS10460 2235449..2236048 + 600 WP_003409989.1 L-lysine exporter -
KURONO_RS10465 2236464..2236892 + 429 WP_003409992.1 cellulose-binding protein -
KURONO_RS10470 2237118..2237657 + 540 WP_003900446.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(37) -
KURONO_RS10475 2238177..2238737 - 561 WP_003410001.1 RES family NAD+ phosphorylase Toxin
KURONO_RS10480 2238734..2239075 - 342 WP_003410003.1 DUF2384 domain-containing protein Antitoxin
KURONO_RS10485 2239161..2239418 + 258 WP_003410006.1 hypothetical protein -
KURONO_RS10490 2239319..2239654 - 336 WP_003410009.1 dehydrogenase -
KURONO_RS10495 2239743..2240087 - 345 WP_003410010.1 type II toxin-antitoxin system toxin endoribonuclease MazF6 -
KURONO_RS10500 2240081..2240329 - 249 WP_003410014.1 ribbon-helix-helix protein, CopG family -
KURONO_RS10505 2240429..2242744 - 2316 WP_003899120.1 cation transporter ATPase CptG -
KURONO_RS10510 2242741..2243013 - 273 WP_003410017.1 DUF1490 family protein -
KURONO_RS10515 2243066..2243422 - 357 WP_003410018.1 Cd(II)/Pb(II)-sensing metalloregulatory transcriptional regulator CmtR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

RES domain

(25-148)

Antitoxin

Antitoxin Xre/MbcA/ParS-like, toxin-binding domain

(69-110)


Sequences

Toxin        


Download         Length: 187 a.a.        Molecular weight: 20248.70 Da        Isoelectric Point: 4.5091

>T30733 WP_003410001.1 NZ_AP014573:c2238737-2238177 [Mycobacterium tuberculosis str. Kurono]
VSDALDEGLVQRIDARGTIEWSETCYRYTGAHRDALSGEGARRFGGRWNPPLLFPAIYLADSAQACMVEVERAAQAASTT
AEKMLEAAYRLHTIDVTDLAVLDLTTPQAREAVGLENDDIYGDDWSGCQAVGHAAWFLHMQGVLVPAAGGVGLVVTAYEQ
RTRPGQLQLRQSVDLTPALYQELRAT

Download         Length: 561 bp

>T30733 NZ_AP014573:c2238737-2238177 [Mycobacterium tuberculosis str. Kurono]
GTGAGCGATGCCCTCGATGAAGGGCTCGTCCAGCGTATCGACGCACGCGGAACAATTGAGTGGTCGGAAACGTGCTACCG
GTATACCGGCGCGCACCGTGACGCCTTGTCCGGTGAGGGCGCGCGCAGATTCGGAGGCAGGTGGAATCCGCCGCTGCTCT
TTCCGGCGATCTATCTTGCTGATTCCGCCCAAGCCTGCATGGTTGAGGTGGAACGGGCGGCGCAAGCGGCTTCAACGACC
GCAGAGAAGATGCTCGAGGCGGCCTACCGACTACACACGATCGACGTCACGGACCTGGCCGTCCTCGATCTGACAACCCC
GCAAGCTCGGGAAGCCGTGGGGCTCGAGAACGACGACATCTATGGCGACGACTGGTCAGGGTGCCAGGCGGTCGGACATG
CGGCCTGGTTCTTGCACATGCAAGGTGTCCTCGTGCCGGCGGCGGGCGGTGTCGGCCTCGTTGTCACCGCGTATGAACAG
CGAACTCGGCCGGGCCAACTACAACTGCGACAAAGCGTCGATCTGACGCCTGCTCTTTACCAAGAACTTCGAGCCACGTA
G

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 12488.33 Da        Isoelectric Point: 4.8359

>AT30733 WP_003410003.1 NZ_AP014573:c2239075-2238734 [Mycobacterium tuberculosis str. Kurono]
MGVNVLASTVSGAIERLGLTYEEVGDIVDASPRSVARWTAGQVVPQRLNKQRLIELAYVADALAEVLPRDQANVWMFSPN
RLLEHRKPADLVRDGEYQRVLALIDAMAEGVFV

Download         Length: 342 bp

>AT30733 NZ_AP014573:c2239075-2238734 [Mycobacterium tuberculosis str. Kurono]
ATGGGAGTAAATGTGCTCGCCTCGACCGTGTCGGGTGCGATCGAGCGCTTGGGATTGACCTACGAGGAAGTCGGTGACAT
CGTCGATGCCTCGCCGCGTTCCGTGGCGCGATGGACCGCAGGTCAGGTGGTTCCCCAACGCCTCAACAAGCAACGACTTA
TCGAGCTGGCCTATGTCGCCGACGCCCTCGCGGAAGTGCTGCCGCGTGACCAGGCGAACGTGTGGATGTTTTCGCCGAAT
CGGTTACTGGAACACCGCAAGCCTGCCGACCTCGTGCGAGACGGCGAGTACCAACGCGTGTTGGCGCTCATCGACGCGAT
GGCGGAGGGAGTGTTCGTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6FKG

Antitoxin

Source ID Structure
PDB 6FKG
AlphaFold DB A0A7U4FAE4

References