Detailed information of TA system TA030729

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2200081..2200784
• Replicon: chromosome
• Accession: NZ_AP014573
• Organism: Mycobacterium tuberculosis str. Kurono

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G0TLK7
• Locus tag: KURONO_RS10215
• Protein ID: WP_003409778.1
• Coordinates: 2200081..2200410 (-)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G0TLK8
• Locus tag: KURONO_RS21355
• Protein ID: WP_003409780.1
• Coordinates: 2200407..2200784 (-)
• Length: 126 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KURONO_RS10195	2196464..2197534	+	1071	WP_003899091.1	epoxide hydrolase EphB	-
KURONO_RS10200	2197531..2198046	+	516	WP_003409718.1	flavin reductase family protein	-
KURONO_RS10205	2198043..2199104	+	1062	WP_003899092.1	3,4-dihydroxy-2-butanone-4-phosphate synthase	-
KURONO_RS10210	2199101..2199871	+	771	WP_003409775.1	SDR family oxidoreductase	-
KURONO_RS10215	2200081..2200410	-	330	WP_003409778.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
KURONO_RS21355	2200407..2200784	-	378	WP_003409780.1	type II toxin-antitoxin system antitoxin MazE5	Antitoxin
KURONO_RS10225	2200781..2201371	-	591	WP_003409784.1	SEC-C domain-containing protein	-
KURONO_RS10230	2201426..2202790	+	1365	WP_003910478.1	HNH endonuclease	-
KURONO_RS10235	2202945..2203397	-	453	WP_003899095.1	lipoprotein	-
KURONO_RS10240	2203461..2203862	+	402	WP_003409869.1	hypothetical protein	-
KURONO_RS10245	2203855..2204037	-	183	WP_003409870.1	hypothetical protein	-
KURONO_RS10250	2204151..2204501	-	351	WP_003899096.1	hypothetical protein	-
KURONO_RS10255	2204512..2205414	-	903	WP_003899097.1	hypothetical protein	-
KURONO_RS10260	2205435..2205626	-	192	WP_003409876.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-103)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 11787.82 Da
• Isoelectric Point: 8.5361

>T30729 WP_003409778.1 NZ_AP014573:c2200410-2200081 [Mycobacterium tuberculosis str. Kurono]
VTALPARGEVWWCEMAEIGRRPVVVLSRDAAIPRLRRALVAPCTTTIRGLASEVVLEPGSDPIPRRSAVNLDSVESVSVA
VLVNRLGRLADIRMRAICTALEVAVDCSR

Nucleotide

• Length: 330 bp

>T30729 NZ_AP014573:c2200410-2200081 [Mycobacterium tuberculosis str. Kurono]
GTGACCGCACTTCCGGCGCGCGGAGAGGTGTGGTGGTGTGAGATGGCTGAGATCGGTCGGCGACCAGTCGTCGTGCTGTC
GCGCGATGCCGCGATCCCTCGGCTGCGACGCGCACTTGTCGCGCCCTGCACCACGACCATCCGAGGGCTAGCCAGTGAGG
TTGTTCTTGAACCCGGTTCCGACCCGATCCCGCGCCGTTCCGCGGTGAATTTGGACTCAGTCGAAAGTGTCTCGGTCGCG
GTATTGGTGAATCGGCTTGGCCGCCTCGCCGACATCCGGATGCGCGCCATCTGCACGGCCCTCGAGGTCGCCGTCGATTG
CTCTCGATGA

Antitoxin

Protein

• Length: 126 a.a.
• Molecular weight: 13560.07 Da
• Isoelectric Point: 5.1519

>AT30729 WP_003409780.1 NZ_AP014573:c2200784-2200407 [Mycobacterium tuberculosis str. Kurono]
VKTARLQVTLRCAVDLINSSSDQCFARIEHVASDQADPRPGVWHSSGMNRIRLSTTVDAALLTSARDMRAGITDAALIDE
ALAALLARHRSAEVDASYAAYDKHPVDEPDEWGDLASWRRAAGDS

Nucleotide

• Length: 378 bp

>AT30729 NZ_AP014573:c2200784-2200407 [Mycobacterium tuberculosis str. Kurono]
GTGAAGACGGCCCGGTTGCAGGTGACGTTGCGCTGCGCGGTAGACCTCATCAATTCGTCCAGCGACCAATGTTTCGCCCG
TATCGAGCACGTTGCGTCCGATCAGGCGGATCCACGCCCCGGAGTGTGGCATAGTAGTGGCATGAATCGCATACGGCTGA
GCACTACTGTCGACGCCGCACTCTTGACAAGTGCGCGCGACATGCGGGCAGGAATCACTGACGCAGCCCTCATCGATGAG
GCCCTTGCGGCATTACTGGCTCGTCATCGATCGGCCGAGGTGGATGCCAGCTATGCGGCCTACGACAAGCACCCGGTCGA
TGAGCCCGACGAGTGGGGCGATCTAGCGTCGTGGCGGCGGGCGGCTGGAGACTCGTGA

Structures

Toxin

• AlphaFold DB: G0TLK7

Antitoxin

• AlphaFold DB: G0TLK8