Detailed information of TA system TA030723

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PhRel-ATphRel/-
• Location: 1543483..1544533
• Replicon: chromosome
• Accession: NZ_AP014573
• Organism: Mycobacterium tuberculosis str. Kurono

Toxin (Protein)

• Gene name: phRel
• Uniprot ID: G0TIH2
• Locus tag: KURONO_RS07265
• Protein ID: WP_003407164.1
• Coordinates: 1543483..1544304 (+)
• Length: 274 a.a.

Antitoxin (Protein)

• Gene name: ATphRel
• Uniprot ID: A0A7U4BTV8
• Locus tag: KURONO_RS07270
• Protein ID: WP_003407167.1
• Coordinates: 1544297..1544533 (+)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KURONO_RS07245	1539041..1539703	-	663	WP_003407152.1	membrane protein	-
KURONO_RS07250	1539700..1540485	-	786	WP_003407157.1	membrane protein	-
KURONO_RS07255	1540776..1542737	-	1962	WP_003916806.1	sigma-F factor regulator	-
KURONO_RS07260	1542876..1543262	-	387	WP_003898845.1	anti-sigma-F factor antagonist RsfA	-
KURONO_RS07265	1543483..1544304	+	822	WP_003407164.1	GTP pyrophosphokinase family protein	Toxin
KURONO_RS07270	1544297..1544533	+	237	WP_003407167.1	hypothetical protein	Antitoxin
KURONO_RS07275	1544605..1545810	-	1206	WP_003898846.1	serine hydrolase	-
KURONO_RS07280	1546113..1546898	+	786	WP_003407180.1	lipoprotein LprF	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RelA/SpoT (76-174)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 274 a.a.
• Molecular weight: 30100.10 Da
• Isoelectric Point: 5.8187

>T30723 WP_003407164.1 NZ_AP014573:1543483-1544304 [Mycobacterium tuberculosis str. Kurono]
VVVALVGSAIVDLHSRPPWSNNAVRRLGVALRDGVDPPVDCPSYAEVMLWHADLAAEVQDRIEGRSWSASELLVTSRAKS
QDTLLAKLRRRPYLQLNTIQDIAGVRIDADLLLGEQTRLAREIADHFGADQPAIHDLRDHPHAGYRAVHVWLRLPAGRVE
IQIRTILQSLWANFYELLADAYGRGIRYDERPEQLAAGVVPAQLQELVGVMQDASADLAMHEAEWQHCAEIEYPGQRAMA
LGEASKNKATVLATTKFRLERAINEAESAGGGG

Nucleotide

• Length: 822 bp

>T30723 NZ_AP014573:1543483-1544304 [Mycobacterium tuberculosis str. Kurono]
GTGGTGGTAGCCCTCGTAGGCTCAGCAATCGTGGACTTGCATTCACGACCACCGTGGTCGAACAACGCGGTGCGTCGTCT
TGGCGTGGCACTGCGCGACGGAGTTGACCCGCCGGTCGACTGCCCGTCGTACGCCGAGGTGATGCTGTGGCATGCGGACT
TGGCCGCCGAAGTCCAGGACCGGATCGAGGGCCGGAGTTGGTCTGCGTCGGAGTTATTGGTTACCTCACGTGCGAAGAGC
CAAGACACCCTGCTAGCAAAGCTGCGGCGTCGGCCTTACCTGCAACTGAACACCATCCAAGACATCGCAGGTGTCCGCAT
CGATGCCGACCTCCTGCTGGGCGAGCAGACGAGACTTGCTCGCGAGATCGCCGACCACTTCGGTGCTGACCAGCCCGCTA
TTCATGATCTGCGTGACCACCCGCACGCCGGCTACCGGGCCGTTCATGTCTGGCTTCGGTTACCTGCCGGTCGTGTCGAG
ATACAGATTCGCACCATTTTGCAGAGCCTGTGGGCCAACTTCTACGAGCTTCTCGCTGACGCGTACGGTCGGGGCATCCG
CTATGACGAGCGGCCGGAGCAGCTAGCGGCCGGCGTTGTCCCGGCACAGCTTCAAGAGCTGGTAGGGGTTATGCAAGACG
CTTCAGCGGATCTGGCGATGCATGAAGCCGAGTGGCAACACTGTGCAGAGATCGAATACCCCGGCCAGCGGGCGATGGCG
CTTGGCGAGGCGAGCAAGAACAAGGCGACGGTGCTCGCAACGACCAAGTTTAGGCTGGAAAGGGCCATCAATGAGGCCGA
GTCGGCAGGGGGAGGTGGGTGA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8830.90 Da
• Isoelectric Point: 7.3178

>AT30723 WP_003407167.1 NZ_AP014573:1544297-1544533 [Mycobacterium tuberculosis str. Kurono]
VGEVAGYVVEYNRRTHVRRITEFATPQEAMEHRLKLEAERTDSNIEIVALVSKSLGTLKQTHSRYFTGEELNVGNGAR

Nucleotide

• Length: 237 bp

>AT30723 NZ_AP014573:1544297-1544533 [Mycobacterium tuberculosis str. Kurono]
GTGGGTGAGGTGGCTGGCTATGTCGTCGAATACAACCGGCGCACCCACGTGCGTCGCATCACCGAGTTCGCCACCCCGCA
AGAAGCGATGGAGCACCGGTTGAAGCTGGAAGCCGAGCGCACCGACAGCAATATCGAGATCGTTGCGCTCGTCAGTAAGT
CGTTGGGAACCCTGAAGCAAACGCATTCGCGGTACTTCACTGGTGAAGAGCTGAACGTCGGAAACGGCGCGCGGTAG

Structures

Toxin

• AlphaFold DB: G0TIH2

Antitoxin

• AlphaFold DB: A0A7U4BTV8