Detailed information of TA system TA030720

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 1242785..1243353
• Replicon: chromosome
• Accession: NZ_AP014573
• Organism: Mycobacterium tuberculosis str. Kurono

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TH08
• Locus tag: KURONO_RS05925
• Protein ID: WP_003405865.1
• Coordinates: 1242979..1243353 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TH07
• Locus tag: KURONO_RS05920
• Protein ID: WP_003405863.1
• Coordinates: 1242785..1242982 (+)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KURONO_RS05900	1238826..1239464	-	639	WP_003898730.1	lipid droplet-associated protein	-
KURONO_RS05905	1239554..1240561	+	1008	WP_003405853.1	4-hydroxy-3-methylbut-2-enyl diphosphate reductase	-
KURONO_RS05910	1240578..1241561	-	984	WP_003898731.1	hypothetical protein	-
KURONO_RS05915	1241624..1242697	+	1074	WP_003898732.1	redox-regulated ATPase YchF	-
KURONO_RS05920	1242785..1242982	+	198	WP_003405863.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
KURONO_RS05925	1242979..1243353	+	375	WP_003405865.1	type II toxin-antitoxin system VapC family toxin	Toxin
KURONO_RS05930	1243556..1244254	+	699	WP_003898733.1	hypothetical protein	-
KURONO_RS05935	1244372..1244557	+	186	WP_003901093.1	hypothetical protein	-
KURONO_RS05940	1244484..1244759	-	276	WP_003405867.1	hypothetical protein	-
KURONO_RS05945	1245002..1245325	+	324	WP_003405871.1	antibiotic biosynthesis monooxygenase	-
KURONO_RS05950	1245340..1246050	-	711	WP_003911399.1	hypothetical protein	-
KURONO_RS22790	1246072..1246281	+	210	WP_003911400.1	hypothetical protein	-
KURONO_RS05960	1246233..1246940	-	708	Protein_1165	adenylate/guanylate cyclase domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-111)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13444.56 Da
• Isoelectric Point: 5.1881

>T30720 WP_003405865.1 NZ_AP014573:1242979-1243353 [Mycobacterium tuberculosis str. Kurono]
VILVDTSVWIEHLRAADARLVELLGDDEAGCHPLVIEELALGSIKQRDVVLDLLANLYQFPVVTHDEVLRLVGRRRLWGR
GLGAVDANLLGSVALVGGARLWTRDKRLKAACAESGVALAEEVS

Nucleotide

• Length: 375 bp

>T30720 NZ_AP014573:1242979-1243353 [Mycobacterium tuberculosis str. Kurono]
GTGATCCTGGTCGACACTTCGGTATGGATTGAGCACCTGCGCGCCGCCGACGCGCGACTCGTCGAGCTGCTGGGCGATGA
CGAGGCCGGTTGCCATCCGCTCGTCATCGAGGAGCTGGCGCTTGGCTCGATCAAGCAGCGAGACGTTGTTCTCGATCTGT
TGGCCAACCTCTACCAGTTTCCGGTGGTGACCCACGACGAAGTGTTGCGGCTTGTCGGTCGGCGGCGGTTGTGGGGTCGG
GGACTCGGTGCCGTCGATGCCAACCTTCTTGGTTCGGTGGCTCTGGTTGGCGGCGCGCGACTATGGACGCGGGACAAGCG
GTTGAAGGCGGCGTGCGCGGAAAGCGGTGTTGCGCTGGCTGAGGAAGTGTCCTGA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 6973.03 Da
• Isoelectric Point: 11.7327

>AT30720 WP_003405863.1 NZ_AP014573:1242785-1242982 [Mycobacterium tuberculosis str. Kurono]
MRTTVTVDDALLAKAAELTGVKEKSTLLREGLQTLVRVESARRLAALGGTDPQATAAPRRRTSPR

Nucleotide

• Length: 198 bp

>AT30720 NZ_AP014573:1242785-1242982 [Mycobacterium tuberculosis str. Kurono]
ATGAGGACGACGGTGACCGTTGACGACGCCTTGTTAGCCAAAGCGGCCGAATTGACTGGGGTGAAAGAGAAGTCGACGCT
CCTGCGCGAGGGGTTGCAGACACTGGTCCGGGTGGAGAGCGCCCGGCGGTTGGCGGCTCTCGGCGGCACCGACCCGCAAG
CTACCGCGGCGCCGAGACGCCGGACGTCGCCCCGGTGA

Structures

Toxin

• AlphaFold DB: G0TH08

Antitoxin

• AlphaFold DB: G0TH07