Detailed information of TA system TA030699

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Rv0298-Rv0299/-
• Location: 367375..367901
• Replicon: chromosome
• Accession: NZ_AP014573
• Organism: Mycobacterium tuberculosis str. Kurono

Toxin (Protein)

• Gene name: Rv0299
• Uniprot ID: -
• Locus tag: KURONO_RS01590
• Protein ID: WP_003401560.1
• Coordinates: 367599..367901 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: Rv0298
• Uniprot ID: P9WJ08
• Locus tag: KURONO_RS01585
• Protein ID: WP_003401555.1
• Coordinates: 367375..367602 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KURONO_RS01570	363068..363871	-	804	WP_003401540.1	hypothetical protein	-
KURONO_RS01575	363881..365278	-	1398	WP_003401544.1	sulfatase	-
KURONO_RS01580	365457..367232	+	1776	WP_010886074.1	PE family protein	-
KURONO_RS01585	367375..367602	+	228	WP_003401555.1	ribbon-helix-helix protein, CopG family	Antitoxin
KURONO_RS01590	367599..367901	+	303	WP_003401560.1	toxin-antitoxin system toxin	Toxin
KURONO_RS01595	367949..368170	+	222	WP_003401563.1	type II toxin-antitoxin system antitoxin VapB2	-
KURONO_RS01600	368167..368592	+	426	WP_003401566.1	PIN domain nuclease	-
KURONO_RS01605	368728..369360	+	633	WP_003401571.1	TetR/AcrR family transcriptional regulator	-
KURONO_RS01610	369357..370265	+	909	WP_003900117.1	SDR family NAD(P)-dependent oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Ribbon-helix-helix protein, CopG (6-41)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 10442.16 Da
• Isoelectric Point: 4.9609

>T30699 WP_003401560.1 NZ_AP014573:367599-367901 [Mycobacterium tuberculosis str. Kurono]
MIAPGDIAPRRDSEHELYVAVLSNALHRAADTGRVITCPFIPGRVPEDLLAMVVAVEQPNGTLLPELVQWLHVAALGAPL
GNAGVAALREAASVVTALLC

Nucleotide

• Length: 303 bp

>T30699 NZ_AP014573:367599-367901 [Mycobacterium tuberculosis str. Kurono]
TTGATCGCTCCCGGCGACATCGCGCCGCGCCGCGACAGTGAACACGAGCTCTACGTCGCCGTCTTGTCCAACGCGCTCCA
TCGGGCCGCGGACACCGGACGGGTGATCACCTGCCCATTCATTCCGGGCCGGGTCCCCGAGGATCTCTTGGCGATGGTGG
TGGCGGTCGAGCAACCCAACGGCACGCTGCTGCCGGAACTCGTGCAGTGGCTTCATGTTGCCGCGCTCGGTGCGCCACTC
GGCAACGCGGGCGTGGCCGCCCTACGCGAGGCTGCCTCGGTCGTGACAGCTCTGCTCTGTTAG

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8130.21 Da
• Isoelectric Point: 7.0084

>AT30699 WP_003401555.1 NZ_AP014573:367375-367602 [Mycobacterium tuberculosis str. Kurono]
MTKEKISVTVDAAVLAAIDADARAAGLNRSEMIEQALRNEHLRVALRDYTAKTVPALDIDAYAQRVYQANRAAGS

Nucleotide

• Length: 228 bp

>AT30699 NZ_AP014573:367375-367602 [Mycobacterium tuberculosis str. Kurono]
ATGACGAAAGAGAAGATCTCCGTGACGGTGGACGCGGCCGTCCTCGCGGCGATCGACGCGGACGCCAGGGCGGCGGGTTT
GAATCGGTCGGAAATGATTGAGCAGGCACTGCGCAACGAGCACCTGCGTGTCGCTCTGCGCGATTACACGGCTAAAACCG
TACCGGCGTTGGACATCGATGCCTACGCACAGCGGGTGTACCAGGCGAACCGGGCGGCCGGAAGTTGA

Structures

Antitoxin

• AlphaFold DB: A0A7U4F8T1