Detailed information of TA system TA030688

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 675456..676297
• Replicon: chromosome
• Accession: NZ_AP014569
• Organism: Serpentinomonas mccroryi strain B1

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: A0A060NKA8
• Locus tag: SMCB_RS03130
• Protein ID: WP_045534993.1
• Coordinates: 675456..676007 (-)
• Length: 184 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: -
• Locus tag: SMCB_RS03135
• Protein ID: WP_171820276.1
• Coordinates: 676004..676297 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SMCB_RS03110	670549..671682	-	1134	WP_045534986.1	Fic family protein	-
SMCB_RS03115	671679..672878	-	1200	WP_045534988.1	restriction endonuclease subunit S	-
SMCB_RS03120	672875..675004	-	2130	WP_045534990.1	type I restriction-modification system subunit M	-
SMCB_RS03125	675168..675419	-	252	Protein_627	cell filamentation protein Fic	-
SMCB_RS03130	675456..676007	-	552	WP_045534993.1	GNAT family N-acetyltransferase	Toxin
SMCB_RS03135	676004..676297	-	294	WP_171820276.1	DUF1778 domain-containing protein	Antitoxin
SMCB_RS03140	676780..679167	-	2388	WP_045534995.1	DUF262 domain-containing protein	-
SMCB_RS03145	679164..680891	-	1728	WP_052468393.1	DUF262 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (41-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (7-96)

Sequences

Toxin

Protein

• Length: 184 a.a.
• Molecular weight: 20269.42 Da
• Isoelectric Point: 8.5186

>T30688 WP_045534993.1 NZ_AP014569:c676007-675456 [Serpentinomonas mccroryi]
VRLRAPEPLAAPHWLEGFDCGKPALNDWLLRHARQAQGSGSAKTFVVTEDDGRVAGYFSLTVGQVDTLEAPERIRKGMGQ
YPLPVVILARLAVSVADQGRGIGFSLLQDAIRRTLLIAEHAGIRAMLTHPIDEEAARFYTRFGFIASPLREQQLLLLLKD
ARRVLMALNELEVKRCQTDLSGQ

Nucleotide

• Length: 552 bp

>T30688 NZ_AP014569:c676007-675456 [Serpentinomonas mccroryi]
GTGAGGTTGCGTGCACCGGAGCCACTGGCCGCGCCGCATTGGCTGGAAGGTTTTGATTGCGGCAAACCCGCGTTGAACGA
CTGGCTGTTGCGTCACGCCCGCCAAGCGCAAGGCAGCGGCTCGGCCAAGACCTTCGTCGTGACTGAGGACGATGGCCGCG
TCGCAGGCTACTTCAGCCTGACCGTGGGGCAAGTCGACACGCTGGAGGCGCCGGAGCGCATCCGCAAGGGAATGGGACAG
TATCCGTTGCCAGTGGTAATCTTGGCGCGGCTCGCTGTCTCGGTAGCGGATCAGGGGCGAGGCATCGGCTTTAGTCTGCT
ACAGGATGCGATTCGCCGCACGCTGCTGATTGCCGAGCACGCTGGCATTCGGGCCATGCTGACCCACCCCATCGATGAAG
AAGCGGCGCGTTTCTACACCCGGTTCGGCTTCATCGCCTCCCCGCTGCGCGAGCAGCAACTCTTGCTGCTGCTCAAGGAT
GCGAGGAGGGTCTTGATGGCCCTGAACGAGTTGGAAGTCAAGCGCTGCCAAACAGATTTATCGGGGCAATGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10777.34 Da
• Isoelectric Point: 5.1297

>AT30688 WP_171820276.1 NZ_AP014569:c676297-676004 [Serpentinomonas mccroryi]
MIALASPPSARSARLGLRATPEQEVVLRRAAEVAHKSLTDFILDSACLAAEQTLLDQRLFMVSGSQYQALLDLLERPEQD
NEGLRELFARKAPWDAK

Nucleotide

• Length: 294 bp

>AT30688 NZ_AP014569:c676297-676004 [Serpentinomonas mccroryi]
ATGATTGCCCTTGCATCGCCTCCTTCCGCCCGCTCTGCCCGACTTGGGCTGCGCGCCACCCCAGAACAAGAGGTGGTGCT
GCGCCGTGCCGCCGAGGTGGCCCACAAGTCGTTGACCGACTTCATCCTCGACAGCGCCTGTTTGGCCGCAGAACAGACGC
TGCTTGATCAGCGCTTGTTCATGGTTTCGGGCAGCCAGTATCAGGCCTTGCTGGATCTGCTGGAGCGCCCCGAACAGGAC
AACGAGGGGCTGCGCGAGTTGTTTGCCCGCAAGGCGCCTTGGGATGCGAAGTGA

Structures

Toxin

• AlphaFold DB: A0A060NKA8