Detailed information of TA system TA030685

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 522125..522705
• Replicon: chromosome
• Accession: NZ_AP014569
• Organism: Serpentinomonas mccroryi strain B1

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: SMCB_RS02340
• Protein ID: WP_197539321.1
• Coordinates: 522364..522705 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A060NMT1
• Locus tag: SMCB_RS02335
• Protein ID: WP_034114635.1
• Coordinates: 522125..522367 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SMCB_RS02315	519168..519761	-	594	WP_144400234.1	pilus assembly protein	-
SMCB_RS02320	519797..520723	-	927	WP_045534766.1	PilW family protein	-
SMCB_RS02325	520738..521337	-	600	WP_052468379.1	type IV pilus modification protein PilV	-
SMCB_RS02330	521334..521942	-	609	WP_082027179.1	GspH/FimT family pseudopilin	-
SMCB_RS02335	522125..522367	+	243	WP_034114635.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
SMCB_RS02340	522364..522705	+	342	WP_197539321.1	endoribonuclease MazF	Toxin
SMCB_RS02345	522735..522986	+	252	WP_045534777.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
SMCB_RS02350	523057..523965	+	909	WP_045534779.1	methyltransferase domain-containing protein	-
SMCB_RS02355	524056..525114	+	1059	WP_045534781.1	Rpn family recombination-promoting nuclease/putative transposase	-
SMCB_RS12335	525172..525336	-	165	WP_082027408.1	type II toxin-antitoxin system YafQ family toxin	-
SMCB_RS02360	525306..525587	-	282	WP_045534784.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	-
SMCB_RS02365	526097..526582	+	486	WP_052468381.1	NYN domain-containing protein	-
SMCB_RS02370	526670..527317	+	648	WP_171820271.1	SCO family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (9-109)

Antitoxin

• Antitoxin PemI-like (6-79)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12082.97 Da
• Isoelectric Point: 10.3155

>T30685 WP_197539321.1 NZ_AP014569:522364-522705 [Serpentinomonas mccroryi]
MMALNVPDEGDIVWLNFTPQSGHEQAGRRPAVVISPKAYNQRSGLLLCVPITSQIKGYPFEVPLSGGGASGAALADQVKS
LDWKSRLAQRKGQATSAELAEIRAKVRALLKLA

Nucleotide

• Length: 342 bp

>T30685 NZ_AP014569:522364-522705 [Serpentinomonas mccroryi]
CTGATGGCGCTGAACGTCCCCGACGAAGGCGACATCGTTTGGCTCAACTTCACGCCCCAAAGCGGACACGAGCAGGCCGG
GCGCCGACCGGCAGTCGTCATCAGCCCCAAGGCGTACAACCAGCGCTCGGGGCTGCTGCTGTGTGTGCCCATCACCAGTC
AGATCAAAGGCTACCCGTTCGAGGTGCCGCTGTCTGGCGGCGGTGCCAGCGGCGCCGCCTTGGCCGACCAAGTCAAGAGC
CTAGACTGGAAGTCGCGCCTAGCCCAGCGCAAGGGCCAAGCCACGAGCGCCGAGTTGGCCGAGATCAGGGCCAAGGTCAG
GGCGCTGCTCAAGCTCGCTTGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8699.96 Da
• Isoelectric Point: 5.6155

>AT30685 WP_034114635.1 NZ_AP014569:522125-522367 [Serpentinomonas mccroryi]
MALQFVKKWGNSPAVRLPAAVMEAAHLRLDQAVEVRAQNGRIVIEPAAPAYDLEALLAGITPQNRHTEQDFGPAQGQELL

Nucleotide

• Length: 243 bp

>AT30685 NZ_AP014569:522125-522367 [Serpentinomonas mccroryi]
ATGGCGCTGCAGTTTGTCAAAAAATGGGGCAACAGCCCGGCCGTGCGCTTGCCTGCAGCGGTGATGGAGGCGGCGCACCT
TAGGCTGGATCAGGCGGTGGAGGTGCGTGCGCAAAACGGCCGCATCGTCATCGAGCCAGCCGCGCCGGCCTACGATCTGG
AGGCGCTGCTAGCCGGCATCACGCCGCAAAACCGGCACACCGAGCAAGACTTTGGGCCAGCGCAAGGGCAGGAGCTGCTC
TGA

Structures

Antitoxin

• AlphaFold DB: A0A060NMT1