Detailed information of TA system TA030671

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-VagC
• Location: 1023504..1024171
• Replicon: chromosome
• Accession: NZ_AP014568
• Organism: Serpentinomonas raichei strain A1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: SRAA_RS04860
• Protein ID: WP_045531255.1
• Coordinates: 1023764..1024171 (+)
• Length: 136 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A060NIR3
• Locus tag: SRAA_RS04855
• Protein ID: WP_045533299.1
• Coordinates: 1023504..1023764 (+)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SRAA_RS04845	1020419..1021150	-	732	WP_045531252.1	GntR family transcriptional regulator	-
SRAA_RS04850	1021255..1023441	+	2187	WP_171820220.1	methylmalonyl-CoA mutase	-
SRAA_RS04855	1023504..1023764	+	261	WP_045533299.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
SRAA_RS04860	1023764..1024171	+	408	WP_045531255.1	type II toxin-antitoxin system VapC family toxin	Toxin
SRAA_RS04865	1024168..1025166	+	999	WP_045531257.1	methylmalonyl Co-A mutase-associated GTPase MeaB	-
SRAA_RS04870	1025174..1026706	+	1533	WP_045531260.1	acyl-CoA carboxylase subunit beta	-
SRAA_RS04875	1026731..1027075	-	345	WP_045531262.1	type II toxin-antitoxin system PemK/MazF family toxin	-
SRAA_RS04880	1027072..1027431	-	360	WP_045531264.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (11-46)

Sequences

Toxin

Protein

• Length: 136 a.a.
• Molecular weight: 15238.58 Da
• Isoelectric Point: 7.5226

>T30671 WP_045531255.1 NZ_AP014568:1023764-1024171 [Serpentinomonas raichei]
MDALYLLDTNMVIYLQRGVPGVQERLAAAGRQRVALPALVVAELAYGVEKSTQQARNRARLEQLLLEMTVLPWTHSAMWH
YARHFHALRQSGQPIGHMDLLIAAQALAENATLVTHNTREFERIEGLKLENWVSA

Nucleotide

• Length: 408 bp

>T30671 NZ_AP014568:1023764-1024171 [Serpentinomonas raichei]
ATGGACGCCTTGTACCTGCTCGACACCAACATGGTGATCTACTTGCAGCGCGGTGTGCCCGGTGTGCAGGAGCGGCTGGC
GGCGGCCGGCCGACAGCGGGTGGCCTTGCCCGCGCTGGTGGTGGCCGAGCTGGCCTATGGTGTAGAAAAAAGCACACAGC
AGGCCCGCAACCGCGCCCGGCTGGAGCAACTGCTGCTGGAAATGACCGTGTTGCCTTGGACGCACAGCGCCATGTGGCAC
TACGCAAGGCATTTTCATGCCCTGCGCCAGAGTGGTCAGCCCATAGGGCATATGGATTTGCTGATTGCCGCCCAAGCGCT
GGCCGAAAACGCCACGCTGGTCACCCACAACACGCGCGAGTTCGAGCGCATCGAGGGCTTGAAACTGGAGAACTGGGTCA
GCGCATGA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9564.73 Da
• Isoelectric Point: 4.6413

>AT30671 WP_045533299.1 NZ_AP014568:1023504-1023764 [Serpentinomonas raichei]
MNTPAHTAKVFTTGRSQAVRLPKAFRFDTDEVTIEKVGSAVILRPKHEDDAVWAARVQSLLAGFEGMPDEIERDRQPAVD
DIESLD

Nucleotide

• Length: 261 bp

>AT30671 NZ_AP014568:1023504-1023764 [Serpentinomonas raichei]
ATGAACACGCCCGCACACACCGCCAAGGTTTTCACCACGGGGCGCAGCCAAGCGGTGCGTCTGCCCAAGGCGTTCCGCTT
TGACACCGACGAGGTGACGATCGAGAAAGTGGGCTCTGCCGTGATCCTTCGCCCCAAGCACGAGGACGACGCCGTGTGGG
CGGCGCGGGTGCAGTCGCTCCTAGCGGGGTTCGAGGGCATGCCTGACGAGATCGAGCGCGACCGCCAGCCCGCTGTCGAT
GACATCGAGAGCCTGGATTGA

Structures

Antitoxin

• AlphaFold DB: A0A060NIR3