Detailed information of TA system TA030670

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 999017..999663
• Replicon: chromosome
• Accession: NZ_AP014568
• Organism: Serpentinomonas raichei strain A1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A060NMR2
• Locus tag: SRAA_RS04740
• Protein ID: WP_045531237.1
• Coordinates: 999253..999663 (+)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: SRAA_RS04735
• Protein ID: WP_082040082.1
• Coordinates: 999017..999253 (+)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SRAA_RS04710	994426..995427	+	1002	WP_082039912.1	glucokinase	-
SRAA_RS04715	995440..996288	-	849	WP_045531233.1	MurR/RpiR family transcriptional regulator	-
SRAA_RS04720	996373..997332	-	960	WP_045531234.1	transaldolase	-
SRAA_RS04725	997356..998195	-	840	WP_045531235.1	shikimate dehydrogenase	-
SRAA_RS04730	998269..998910	+	642	WP_052467488.1	HNH endonuclease family protein	-
SRAA_RS04735	999017..999253	+	237	WP_082040082.1	antitoxin	Antitoxin
SRAA_RS04740	999253..999663	+	411	WP_045531237.1	tRNA(fMet)-specific endonuclease VapC	Toxin
SRAA_RS04745	999904..1000788	+	885	WP_144318706.1	dioxygenase	-
SRAA_RS04750	1000841..1001251	-	411	WP_045531239.1	PaaI family thioesterase	-
SRAA_RS04755	1001410..1002483	+	1074	WP_045531240.1	asparaginase	-
SRAA_RS04760	1002502..1003158	-	657	WP_045531241.1	adenylate kinase	-
SRAA_RS04765	1003276..1004067	-	792	WP_034111657.1	3-deoxy-manno-octulosonate cytidylyltransferase	-
SRAA_RS04770	1004084..1004302	-	219	WP_045531242.1	Trm112 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-123)

Antitoxin

• SpoVT-AbrB domain (7-56)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 14896.09 Da
• Isoelectric Point: 6.8716

>T30670 WP_045531237.1 NZ_AP014568:999253-999663 [Serpentinomonas raichei]
MLRYLLDTNIVIYVLKRRPVEVLSTFNANASRIAISSITLAELLHGAEKSSRVSENLSAIEDFCSRLAVLPYGAKAAQHY
GAIRDALEKLGQPIGVNDLHIAGHARSEGMVLVSNNVSEFARVPALEVENWVRAPE

Nucleotide

• Length: 411 bp

>T30670 NZ_AP014568:999253-999663 [Serpentinomonas raichei]
ATGCTGCGCTACCTGCTTGACACCAACATCGTCATCTACGTGTTGAAACGACGCCCGGTCGAGGTTCTGTCGACCTTCAA
CGCCAATGCCAGTCGAATAGCCATTTCTTCCATTACCCTGGCCGAACTCTTGCACGGCGCCGAAAAAAGCAGCCGCGTGA
GCGAGAACTTGTCTGCCATCGAGGACTTCTGCAGCCGCTTGGCCGTGTTGCCCTATGGCGCCAAGGCCGCACAGCACTAT
GGTGCCATTCGAGACGCCCTGGAAAAACTCGGTCAACCCATCGGGGTGAATGACCTGCACATTGCCGGCCACGCGCGCAG
TGAGGGGATGGTTTTGGTGAGCAATAACGTTTCGGAATTTGCGCGCGTTCCTGCGCTCGAGGTAGAGAATTGGGTCCGCG
CCCCAGAGTAA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8779.00 Da
• Isoelectric Point: 7.2040

>AT30670 WP_082040082.1 NZ_AP014568:999017-999253 [Serpentinomonas raichei]
MSIGTVFVNNRTQAVRLPLDVRLPEGVHKVEIRAKGNERIIAPLGQTWDSFFLGGPPQVSEDFLPQRATQHQSEREVL

Nucleotide

• Length: 237 bp

>AT30670 NZ_AP014568:999017-999253 [Serpentinomonas raichei]
ATGTCTATCGGCACTGTGTTTGTCAATAACCGCACCCAAGCCGTGCGCCTCCCATTGGACGTTCGCCTGCCCGAGGGTGT
ACACAAAGTAGAGATACGGGCCAAGGGTAACGAGCGCATCATCGCGCCCCTAGGTCAAACGTGGGACAGCTTCTTCCTGG
GTGGCCCGCCCCAAGTCAGTGAAGACTTCTTGCCTCAACGTGCCACGCAGCACCAGTCGGAGCGGGAAGTGCTCTGA

Structures

Toxin

• AlphaFold DB: A0A060NMR2