Detailed information of TA system TA030669

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/upstrm_HI1419-dnstrm_HI1420
• Location: 526280..526870
• Replicon: chromosome
• Accession: NZ_AP014568
• Organism: Serpentinomonas raichei strain A1

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A060NHW5
• Locus tag: SRAA_RS02580
• Protein ID: WP_045530765.1
• Coordinates: 526280..526579 (+)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A060NFV2
• Locus tag: SRAA_RS02585
• Protein ID: WP_045530766.1
• Coordinates: 526583..526870 (+)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SRAA_RS12590	521433..521588	+	156	WP_171820213.1	hypothetical protein	-
SRAA_RS02540	521686..522006	-	321	WP_045530758.1	putative addiction module antidote protein	-
SRAA_RS02545	522016..522306	-	291	WP_045530759.1	type II toxin-antitoxin system RelE/ParE family toxin	-
SRAA_RS02550	522364..522588	-	225	WP_045530760.1	type II toxin-antitoxin system HicA family toxin	-
SRAA_RS02555	522588..522797	-	210	WP_045530761.1	type II toxin-antitoxin system HicB family antitoxin	-
SRAA_RS02560	523199..523486	-	288	WP_045530762.1	HigA family addiction module antidote protein	-
SRAA_RS02565	523568..524008	+	441	WP_045530763.1	hypothetical protein	-
SRAA_RS02570	524001..525332	+	1332	WP_045530764.1	hypothetical protein	-
SRAA_RS02575	525393..526232	+	840	WP_082039864.1	CDP-diacylglycerol--serine O-phosphatidyltransferase	-
SRAA_RS02580	526280..526579	+	300	WP_045530765.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
SRAA_RS02585	526583..526870	+	288	WP_045530766.1	putative addiction module antidote protein	Antitoxin
SRAA_RS02590	527130..528680	+	1551	WP_045530767.1	2-isopropylmalate synthase	-
SRAA_RS02595	529127..530116	+	990	WP_144318765.1	serine hydrolase	-
SRAA_RS02600	530140..530946	-	807	WP_045530768.1	IclR family transcriptional regulator	-
SRAA_RS02605	530999..531751	-	753	WP_045530769.1	arginyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	517547..526870	9323

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (4-94)

Antitoxin

• Uncharacterized protein HI1420 (6-93)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11198.74 Da
• Isoelectric Point: 9.6229

>T30669 WP_045530765.1 NZ_AP014568:526280-526579 [Serpentinomonas raichei]
MNTFERSEQFDAWLLGLKDKLGRARIALRIRSAEHGNFGDCEPVGGGVSEMRVHVGPGYRAYFTRRAERTYLLLVGGDKA
SQKRDIKRAIEMARGLDEE

Nucleotide

• Length: 300 bp

>T30669 NZ_AP014568:526280-526579 [Serpentinomonas raichei]
ATGAACACCTTTGAGCGCTCAGAGCAGTTTGATGCTTGGCTCTTAGGTCTGAAGGACAAATTGGGCCGCGCCCGGATCGC
CCTGCGCATTCGGTCTGCCGAGCACGGAAATTTCGGCGACTGTGAGCCGGTTGGTGGCGGTGTTTCCGAAATGCGCGTCC
ACGTTGGGCCGGGCTACCGGGCCTATTTCACACGGCGTGCAGAGCGGACTTATTTGCTTCTTGTGGGGGGCGACAAGGCC
TCGCAGAAGCGTGACATAAAGCGTGCCATCGAGATGGCGCGGGGCTTGGATGAGGAGTAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10222.68 Da
• Isoelectric Point: 4.7778

>AT30669 WP_045530766.1 NZ_AP014568:526583-526870 [Serpentinomonas raichei]
MAKFATFDAADYLDDEETIALYISAALEDPNPEVFLTAVRDVARARGMAQLAKDAGLGRESLYEALAPGAKPRYDTMLKL
LHALGVKLSASPVHS

Nucleotide

• Length: 288 bp

>AT30669 NZ_AP014568:526583-526870 [Serpentinomonas raichei]
ATGGCCAAATTTGCCACTTTTGATGCGGCCGACTACCTCGACGATGAGGAAACCATTGCGCTATACATCAGTGCTGCGCT
GGAAGATCCCAATCCCGAAGTGTTTTTAACTGCGGTGCGTGATGTGGCTCGCGCCCGTGGCATGGCACAACTTGCCAAGG
ATGCGGGCCTTGGCCGCGAGAGCCTTTACGAAGCCCTTGCGCCAGGCGCCAAGCCACGCTACGACACCATGCTCAAGCTG
CTTCATGCGCTGGGTGTGAAGCTTTCTGCTTCCCCCGTCCACTCATGA

Structures

Toxin

• AlphaFold DB: A0A060NHW5

Antitoxin

• AlphaFold DB: A0A060NFV2