Detailed information of TA system TA030668

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA(toxin)
• Location: 522364..522797
• Replicon: chromosome
• Accession: NZ_AP014568
• Organism: Serpentinomonas raichei strain A1

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: SRAA_RS02550
• Protein ID: WP_045530760.1
• Coordinates: 522364..522588 (-)
• Length: 75 a.a.

Antitoxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: SRAA_RS02555
• Protein ID: WP_045530761.1
• Coordinates: 522588..522797 (-)
• Length: 70 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SRAA_RS02510	517547..517804	+	258	WP_045530753.1	DUF2442 domain-containing protein	-
SRAA_RS02515	517916..518134	+	219	WP_082040072.1	type II toxin-antitoxin system RelE/ParE family toxin	-
SRAA_RS02520	518097..518417	+	321	WP_045530755.1	DNA-binding transcriptional regulator	-
SRAA_RS02525	518539..519990	-	1452	WP_052467456.1	O-antigen ligase family protein	-
SRAA_RS02530	520079..520504	-	426	WP_045530756.1	pilin	-
SRAA_RS02535	520717..521142	-	426	WP_045530757.1	pilin	-
SRAA_RS12590	521433..521588	+	156	WP_171820213.1	hypothetical protein	-
SRAA_RS02540	521686..522006	-	321	WP_045530758.1	putative addiction module antidote protein	-
SRAA_RS02545	522016..522306	-	291	WP_045530759.1	type II toxin-antitoxin system RelE/ParE family toxin	-
SRAA_RS02550	522364..522588	-	225	WP_045530760.1	type II toxin-antitoxin system HicA family toxin	Toxin
SRAA_RS02555	522588..522797	-	210	WP_045530761.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
SRAA_RS02560	523199..523486	-	288	WP_045530762.1	HigA family addiction module antidote protein	-
SRAA_RS02565	523568..524008	+	441	WP_045530763.1	hypothetical protein	-
SRAA_RS02570	524001..525332	+	1332	WP_045530764.1	hypothetical protein	-
SRAA_RS02575	525393..526232	+	840	WP_082039864.1	CDP-diacylglycerol--serine O-phosphatidyltransferase	-
SRAA_RS02580	526280..526579	+	300	WP_045530765.1	type II toxin-antitoxin system RelE/ParE family toxin	-
SRAA_RS02585	526583..526870	+	288	WP_045530766.1	putative addiction module antidote protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	517547..526870	9323

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (10-64)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 75 a.a.
• Molecular weight: 8390.71 Da
• Isoelectric Point: 10.9132

>T30668 WP_045530760.1 NZ_AP014568:c522588-522364 [Serpentinomonas raichei]
MGSLPVLKPQEVVRHLERLGFAESRQKGSHKQFRHPDGRATTVPFHKGQDISPVLLRKIAQDINVLVEELLGRQ

Nucleotide

• Length: 225 bp

>T30668 NZ_AP014568:c522588-522364 [Serpentinomonas raichei]
ATGGGCAGCCTGCCCGTTTTGAAGCCGCAGGAAGTTGTTCGCCATCTCGAACGTCTGGGTTTCGCTGAGTCTAGGCAGAA
GGGTTCGCACAAGCAGTTCCGACACCCCGACGGTCGAGCAACAACCGTCCCGTTTCACAAGGGCCAAGATATTTCACCCG
TCTTGCTTCGCAAAATTGCGCAAGACATCAACGTATTGGTCGAAGAGTTGCTGGGCCGGCAGTAG

Antitoxin

Protein

• Length: 70 a.a.
• Molecular weight: 7459.50 Da
• Isoelectric Point: 3.8099

>AT30668 WP_045530761.1 NZ_AP014568:c522797-522588 [Serpentinomonas raichei]
MKTFTAIVEKCMETGLYVGYVPGFPGAHTQGTSLDELQQNLQEVVSMLLEDGEPVLDAQFVGTQQLAIA

Nucleotide

• Length: 210 bp

>AT30668 NZ_AP014568:c522797-522588 [Serpentinomonas raichei]
ATGAAAACTTTTACCGCCATAGTAGAAAAGTGCATGGAAACAGGGCTTTACGTTGGTTATGTCCCCGGATTTCCGGGCGC
GCATACCCAAGGCACCTCACTGGATGAGTTGCAGCAAAATCTCCAAGAGGTTGTCTCCATGCTCTTGGAAGATGGCGAGC
CGGTGCTTGATGCACAGTTTGTGGGCACCCAGCAGCTTGCCATCGCGTAA