Detailed information of TA system TA030666

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yoeB-relB/Txe-YefM
• Location: 516768..517270
• Replicon: chromosome
• Accession: NZ_AP014568
• Organism: Serpentinomonas raichei strain A1

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: A0A060NLJ9
• Locus tag: SRAA_RS02500
• Protein ID: WP_045530751.1
• Coordinates: 517007..517270 (+)
• Length: 88 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: SRAA_RS02495
• Protein ID: WP_045533140.1
• Coordinates: 516768..517010 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SRAA_RS02470	513320..513610	-	291	WP_045530746.1	co-chaperone GroES	-
SRAA_RS02475	513718..514209	-	492	WP_045530747.1	type II toxin-antitoxin system YhaV family toxin	-
SRAA_RS02480	514206..514535	-	330	WP_045530748.1	type II toxin-antitoxin system PrlF family antitoxin	-
SRAA_RS02485	514599..515156	-	558	WP_045530749.1	1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD	-
SRAA_RS02490	515269..516453	-	1185	WP_045530750.1	ATP-binding protein	-
SRAA_RS02495	516768..517010	+	243	WP_045533140.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
SRAA_RS02500	517007..517270	+	264	WP_045530751.1	Txe/YoeB family addiction module toxin	Toxin
SRAA_RS02505	517336..517575	+	240	WP_045530752.1	DUF4160 domain-containing protein	-
SRAA_RS02510	517547..517804	+	258	WP_045530753.1	DUF2442 domain-containing protein	-
SRAA_RS02515	517916..518134	+	219	WP_082040072.1	type II toxin-antitoxin system RelE/ParE family toxin	-
SRAA_RS02520	518097..518417	+	321	WP_045530755.1	DNA-binding transcriptional regulator	-
SRAA_RS02525	518539..519990	-	1452	WP_052467456.1	O-antigen ligase family protein	-
SRAA_RS02530	520079..520504	-	426	WP_045530756.1	pilin	-
SRAA_RS02535	520717..521142	-	426	WP_045530757.1	pilin	-
SRAA_RS12590	521433..521588	+	156	WP_171820213.1	hypothetical protein	-
SRAA_RS02540	521686..522006	-	321	WP_045530758.1	putative addiction module antidote protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (7-86)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-69)

Sequences

Toxin

Protein

• Length: 88 a.a.
• Molecular weight: 10032.59 Da
• Isoelectric Point: 9.8059

>T30666 WP_045530751.1 NZ_AP014568:517007-517270 [Serpentinomonas raichei]
VKWAVVYARQALKDAQKISAAGLKEKAQALLEILATDPFQNPPADEKLVGDLEGAYSRRINIQHRLVYEVLKQERTVRVL
RMWSHYE

Nucleotide

• Length: 264 bp

>T30666 NZ_AP014568:517007-517270 [Serpentinomonas raichei]
GTGAAGTGGGCCGTTGTCTATGCCAGGCAGGCACTGAAGGATGCCCAAAAAATCTCGGCCGCCGGGCTGAAGGAAAAGGC
GCAAGCGCTGCTCGAAATCTTGGCCACCGATCCCTTTCAAAACCCGCCCGCCGACGAAAAGCTGGTGGGCGATCTCGAAG
GGGCCTACTCGCGCCGAATCAACATCCAGCACCGCCTAGTTTACGAGGTGCTCAAGCAGGAACGCACCGTGCGTGTGCTG
CGCATGTGGTCGCATTACGAATGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8594.87 Da
• Isoelectric Point: 5.6862

>AT30666 WP_045533140.1 NZ_AP014568:516768-517010 [Serpentinomonas raichei]
MTPITASAARADLYRLIDQAAQSHQPILISGKRSSAVLVSAADWQAIQETLYLLSVPGMRESILQGMAEPLGQSAKAVDW

Nucleotide

• Length: 243 bp

>AT30666 NZ_AP014568:516768-517010 [Serpentinomonas raichei]
CTGACCCCCATCACCGCAAGCGCAGCGCGTGCCGATCTGTACCGCCTGATCGATCAGGCGGCTCAATCGCATCAGCCGAT
CTTGATTTCTGGCAAGCGCAGCAGCGCGGTCTTGGTTTCGGCCGCTGACTGGCAAGCCATACAAGAGACGCTCTACCTGC
TGTCGGTGCCGGGCATGCGCGAGTCCATCTTGCAGGGCATGGCCGAGCCCCTAGGCCAGAGTGCCAAGGCGGTTGACTGG
TGA

Structures

Toxin

• AlphaFold DB: A0A060NLJ9