Detailed information of TA system TA030662

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-DUF971
• Location: 369722..370314
• Replicon: chromosome
• Accession: NZ_AP014568
• Organism: Serpentinomonas raichei strain A1

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: A0A060NFJ0
• Locus tag: SRAA_RS01765
• Protein ID: WP_045530638.1
• Coordinates: 369722..370018 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: SRAA_RS01770
• Protein ID: WP_197538513.1
• Coordinates: 370015..370314 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SRAA_RS01740	365029..365688	+	660	WP_045530630.1	CoA pyrophosphatase	-
SRAA_RS01745	365790..366731	+	942	WP_045530632.1	CobD/CbiB family protein	-
SRAA_RS01750	366751..367719	-	969	WP_171820210.1	ribosome small subunit-dependent GTPase A	-
SRAA_RS01755	367716..368987	-	1272	WP_045530634.1	M48 family metallopeptidase	-
SRAA_RS01760	369068..369649	+	582	WP_144318682.1	oligoribonuclease	-
SRAA_RS01765	369722..370018	+	297	WP_045530638.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
SRAA_RS01770	370015..370314	+	300	WP_197538513.1	putative addiction module antidote protein	Antitoxin
SRAA_RS01775	370639..372384	+	1746	WP_082039845.1	DEAD/DEAH box helicase	-
SRAA_RS01780	372531..373400	+	870	WP_082040068.1	MotA/TolQ/ExbB proton channel family protein	-
SRAA_RS01785	373397..374281	+	885	WP_197538514.1	OmpA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (1-95)

Antitoxin

• Uncharacterized protein HI1420 (6-93)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11115.75 Da
• Isoelectric Point: 10.6053

>T30662 WP_045530638.1 NZ_AP014568:369722-370018 [Serpentinomonas raichei]
MFELLQTEAFRKWRSRLKDGRARTAIAARLDRLAFGHVGDAEPVGKGVSELRIHYGPGYRVYFQRRGNQIIVLLCGGDKA
SQVRDIATALRLAEQWSE

Nucleotide

• Length: 297 bp

>T30662 NZ_AP014568:369722-370018 [Serpentinomonas raichei]
ATGTTTGAGTTGCTGCAAACCGAAGCCTTTCGCAAGTGGCGTTCTAGGCTCAAGGATGGGCGCGCTCGCACCGCCATTGC
AGCGCGGCTCGATCGCTTGGCCTTTGGTCATGTGGGCGACGCCGAGCCAGTGGGTAAGGGTGTGAGCGAACTGCGCATCC
ACTATGGTCCGGGGTACCGGGTGTATTTTCAGCGGCGCGGCAATCAGATCATCGTTTTGTTGTGCGGCGGTGACAAAGCC
TCGCAGGTGCGGGACATTGCTACCGCGCTGCGCCTGGCAGAGCAATGGAGTGAATGA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10478.89 Da
• Isoelectric Point: 6.7209

>AT30662 WP_197538513.1 NZ_AP014568:370015-370314 [Serpentinomonas raichei]
MSMSEKLSPYDPAEDLSSDQAIADFMAAALQTQDTAYISHALGVVARAKGMAQIANQTGLSREQLYRSFSAHGNPTLRST
LAVMKALGIELSAKPVAGH

Nucleotide

• Length: 300 bp

>AT30662 NZ_AP014568:370015-370314 [Serpentinomonas raichei]
ATGAGCATGAGCGAAAAACTGAGCCCCTACGATCCGGCCGAGGATTTGAGTTCCGACCAGGCCATTGCTGACTTCATGGC
TGCGGCGCTCCAGACCCAGGATACGGCCTACATCTCGCATGCGCTGGGCGTCGTTGCCCGGGCCAAGGGCATGGCGCAGA
TTGCCAACCAGACCGGCCTGTCGCGCGAACAGCTCTACCGCTCCTTCAGCGCCCATGGCAACCCGACGCTGCGCTCCACG
CTGGCTGTTATGAAGGCGCTAGGGATCGAGCTATCCGCAAAGCCGGTTGCGGGTCACTAA

Structures

Toxin

• AlphaFold DB: A0A060NFJ0