Detailed information of TA system TA030661

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 336213..336793
• Replicon: chromosome
• Accession: NZ_AP014568
• Organism: Serpentinomonas raichei strain A1

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: SRAA_RS01570
• Protein ID: WP_144318680.1
• Coordinates: 336452..336793 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A060NFF9
• Locus tag: SRAA_RS01565
• Protein ID: WP_045530605.1
• Coordinates: 336213..336455 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SRAA_RS01530	331248..331547	-	300	WP_045530594.1	HigA family addiction module antidote protein	-
SRAA_RS01535	331561..331842	-	282	WP_045530596.1	type II toxin-antitoxin system RelE/ParE family toxin	-
SRAA_RS01540	332076..333155	+	1080	WP_171820207.1	porin	-
SRAA_RS11980	333544..334557	+	1014	WP_171820208.1	porin	-
SRAA_RS01550	334895..335143	+	249	WP_197538523.1	BrnA antitoxin family protein	-
SRAA_RS01555	335325..335603	-	279	WP_045530601.1	XRE family transcriptional regulator	-
SRAA_RS01560	335600..335947	-	348	WP_045530603.1	type II toxin-antitoxin system RelE/ParE family toxin	-
SRAA_RS01565	336213..336455	+	243	WP_045530605.1	PbsX family transcriptional regulator	Antitoxin
SRAA_RS01570	336452..336793	+	342	WP_144318680.1	endoribonuclease MazF	Toxin
SRAA_RS11985	336997..337713	+	717	WP_052467439.1	hypothetical protein	-
SRAA_RS01580	337798..339006	-	1209	WP_045530610.1	ATP-binding protein	-
SRAA_RS01585	339274..339564	+	291	WP_045533055.1	nucleotidyltransferase family protein	-
SRAA_RS01590	339561..339949	+	389	Protein_327	DUF86 domain-containing protein	-
SRAA_RS01595	339982..341154	-	1173	WP_171820209.1	O-antigen ligase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (5-112)

Antitoxin

• Antitoxin PemI-like (6-79)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12164.98 Da
• Isoelectric Point: 8.9403

>T30661 WP_144318680.1 NZ_AP014568:336452-336793 [Serpentinomonas raichei]
MMATYVPDEGDIVWLSFTPQSGHEQAGRRPAVVLSAKAYNQRSGLLVCVPITNQIKGYPFEVALGGDGATGAALADQVKS
LDWESRQAERKGRATPPELAEIKAKIKALLKLT

Nucleotide

• Length: 342 bp

>T30661 NZ_AP014568:336452-336793 [Serpentinomonas raichei]
CTGATGGCGACGTACGTGCCGGATGAGGGCGACATCGTCTGGTTGAGTTTTACGCCGCAAAGCGGACACGAGCAAGCCGG
TAGGCGACCGGCTGTGGTTCTGAGCGCCAAGGCCTACAACCAGCGCTCGGGCTTGCTGGTCTGCGTCCCGATCACCAACC
AGATCAAGGGCTATCCCTTTGAAGTGGCGCTCGGTGGCGACGGTGCCACCGGGGCCGCCCTGGCTGATCAGGTCAAGAGC
CTGGACTGGGAATCCCGCCAAGCCGAACGCAAGGGGCGGGCCACACCCCCAGAGCTCGCCGAGATCAAGGCCAAGATCAA
GGCCTTGCTCAAGCTTACCTGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8759.13 Da
• Isoelectric Point: 7.2023

>AT30661 WP_045530605.1 NZ_AP014568:336213-336455 [Serpentinomonas raichei]
MALQLIKKWGNSPAIRLPAAVMQAAHLRLEQAVDIRVENGRVVIDAAAPSYKLDDLLAGITAQNRHGEQDFGMPQGRELL

Nucleotide

• Length: 243 bp

>AT30661 NZ_AP014568:336213-336455 [Serpentinomonas raichei]
ATGGCACTGCAGCTAATCAAAAAATGGGGCAACAGCCCGGCCATACGCTTGCCCGCAGCGGTGATGCAGGCCGCACACCT
GAGGCTTGAGCAAGCAGTGGACATTCGCGTCGAGAACGGTCGCGTCGTCATTGATGCCGCAGCGCCGTCCTACAAGCTGG
ACGATCTGCTGGCAGGGATCACGGCCCAAAATCGGCACGGTGAGCAGGACTTCGGGATGCCGCAGGGCCGGGAGTTGCTC
TGA

Structures

Antitoxin

• AlphaFold DB: A0A060NFF9