Detailed information of TA system TA030655

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): unclassified/Polyketide_cyc2(toxin)
• Location: 2513816..2514528
• Replicon: chromosome
• Accession: NZ_AP014567
• Organism: Mycobacterium leprae Kyoto-2

Toxin (Protein)

• Gene name: novel[antitoxin]
• Uniprot ID: -
• Locus tag: JK2ML_RS11875
• Protein ID: WP_197492508.1
• Coordinates: 2513816..2514340 (-)
• Length: 175 a.a.

Antitoxin (Protein)

• Gene name: novel[toxin]
• Uniprot ID: Q9CBE3
• Locus tag: JK2ML_RS11880
• Protein ID: WP_010908719.1
• Coordinates: 2514358..2514528 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JK2ML_RS11855	2510257..2510493	+	237	WP_049769854.1	hypothetical protein	-
JK2ML_RS11860	2510808..2512281	+	1474	Protein_2084	carotenoid oxygenase family protein	-
JK2ML_RS11865	2512485..2512919	-	435	WP_010908717.1	hypothetical protein	-
JK2ML_RS11870	2512978..2513746	-	769	Protein_2086	VOC family protein	-
JK2ML_RS11875	2513816..2514340	-	525	WP_197492508.1	SRPBCC family protein	Toxin
JK2ML_RS11880	2514358..2514528	-	171	WP_010908719.1	antitoxin	Antitoxin
JK2ML_RS11885	2514634..2516644	-	2011	Protein_2089	hypothetical protein	-
JK2ML_RS11890	2516641..2517490	-	850	Protein_2090	hypothetical protein	-
JK2ML_RS11895	2517595..2518522	-	928	Protein_2091	MBL fold metallo-hydrolase	-
JK2ML_RS11900	2518716..2519459	-	744	WP_119608000.1	enoyl-CoA hydratase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polyketide cyclase/dehydrase (1-141)

Antitoxin

• Antitoxin Rv0909/MT0933 (2-50)

Sequences

Toxin

Protein

• Length: 175 a.a.
• Molecular weight: 18906.92 Da
• Isoelectric Point: 9.9666

>T30655 WP_197492508.1 NZ_AP014567:c2514340-2513816 [Mycobacterium leprae Kyoto-2]
MAKLTGSIDVPLPPNEAWMCASDLARFGEWLTIHRAWRSKLPEVVEKGTVIESYVEVKGMPNRIRWTVVRYKAPEAMTLN
GDGVGGVKVKLIAKVSPKDDGSVVSFDVHLGGSALFGPIGMIVAVALRTDIRESLQNFVTAFSRPEPGLIRSRALVVDQH
SRAVVEQRSASAGL

Nucleotide

• Length: 525 bp

>T30655 NZ_AP014567:c2514340-2513816 [Mycobacterium leprae Kyoto-2]
ATGGCGAAACTCACCGGATCTATCGACGTACCACTGCCACCGAATGAGGCCTGGATGTGTGCCTCCGATCTGGCCCGCTT
CGGTGAATGGCTAACGATTCACCGGGCGTGGCGCAGCAAGTTGCCTGAAGTGGTGGAGAAGGGCACGGTCATCGAGTCCT
ATGTCGAGGTCAAGGGCATGCCCAACCGGATCAGATGGACGGTTGTGCGTTACAAAGCGCCTGAGGCCATGACGCTCAAC
GGCGACGGTGTTGGCGGTGTCAAGGTCAAGCTGATTGCTAAGGTTTCGCCTAAGGACGACGGCTCTGTCGTCAGCTTTGA
TGTGCATCTGGGTGGCTCCGCGCTGTTTGGACCGATAGGGATGATCGTCGCCGTTGCGCTGCGAACTGATATCCGCGAAT
CGTTACAGAACTTTGTCACGGCGTTCTCGCGACCGGAACCTGGCCTGATTAGGAGTCGGGCGCTCGTCGTTGACCAGCAT
AGTAGGGCCGTCGTCGAACAGCGGTCAGCATCGGCCGGGCTGTGA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6182.93 Da
• Isoelectric Point: 4.4883

>AT30655 WP_010908719.1 NZ_AP014567:c2514528-2514358 [Mycobacterium leprae Kyoto-2]
VLDKAKDILVQNADKVETVLDKAGEFVDEKAKGKYTDTIYQVAEETKKAASFDTFG

Nucleotide

• Length: 171 bp

>AT30655 NZ_AP014567:c2514528-2514358 [Mycobacterium leprae Kyoto-2]
GTGCTGGACAAAGCAAAGGACATACTGGTGCAGAATGCTGACAAAGTCGAAACGGTGCTCGATAAGGCCGGCGAGTTCGT
CGACGAAAAGGCGAAAGGCAAGTACACTGACACTATCTATCAGGTAGCTGAAGAGACCAAGAAGGCCGCTAGCTTTGACA
CTTTTGGGTGA

Structures

Antitoxin

• AlphaFold DB: A0A197SER5