Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-YafN
Location 430821..431349 Replicon chromosome
Accession NZ_AP014525
Organism Vibrio cholerae MS6

Toxin (Protein)


Gene name relE Uniprot ID A0A0X1KZS6
Locus tag MS6_RS15835 Protein ID WP_000221354.1
Coordinates 431062..431349 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID A0A0X1KZS8
Locus tag MS6_RS15830 Protein ID WP_001250179.1
Coordinates 430821..431072 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MS6_RS15790 426089..426403 + 315 WP_000071008.1 DNA-binding transcriptional regulator -
MS6_RS15795 426540..426974 + 435 WP_000256036.1 GNAT family N-acetyltransferase -
MS6_RS20445 427142..427297 + 156 WP_000751734.1 hypothetical protein -
MS6_RS15805 427422..428402 - 981 WP_041051966.1 IS5-like element ISVch5 family transposase -
MS6_RS15810 428470..428776 + 307 Protein_465 CatB-related O-acetyltransferase -
MS6_RS15815 428913..429605 + 693 WP_001047177.1 GNAT family N-acetyltransferase -
MS6_RS15820 429605..430006 + 402 WP_000351248.1 type II toxin-antitoxin system death-on-curing family toxin -
MS6_RS20245 429976..430119 + 144 WP_071908341.1 DUF3265 domain-containing protein -
MS6_RS15825 430194..430607 + 414 WP_000049417.1 VOC family protein -
MS6_RS15830 430821..431072 + 252 WP_001250179.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
MS6_RS15835 431062..431349 + 288 WP_000221354.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
MS6_RS15840 431477..431932 + 456 WP_001245327.1 GNAT family N-acetyltransferase -
MS6_RS15845 432254..432406 + 153 WP_001884520.1 DUF645 family protein -
MS6_RS15850 432602..433270 + 669 WP_000043871.1 hypothetical protein -
MS6_RS15855 433422..433709 + 288 WP_000426470.1 hypothetical protein -
MS6_RS15860 433850..434221 + 372 WP_001164080.1 nucleotide pyrophosphohydrolase -
MS6_RS20450 434288..434713 + 426 WP_001882332.1 DUF1778 domain-containing protein -
MS6_RS15870 434710..435243 + 534 WP_000118351.1 GNAT family N-acetyltransferase -
MS6_RS15875 435445..435702 + 258 WP_000861987.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
MS6_RS15880 435690..435992 + 303 WP_000229317.1 type II toxin-antitoxin system RelE/ParE family toxin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island qnrVC4 - 297729..445581 147852
- inside Integron qnrVC4 - 302809..445205 142396
- inside IScluster/Tn - - 423729..428402 4673


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(11-71)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 10991.96 Da        Isoelectric Point: 10.4934

>T30646 WP_000221354.1 NZ_AP014525:431062-431349 [Vibrio cholerae MS6]
MTYKLKFLPAAQKEWSKLAPTIQSQFKKKLKERLENPHVPSAKLRGYDAVYKIKLRTAGYRLAYEVIDDEIVVYVLAVGK
RDKDAVYKKLASRFG

Download         Length: 288 bp

>T30646 NZ_AP014525:431062-431349 [Vibrio cholerae MS6]
ATGACCTATAAGTTAAAGTTTCTGCCGGCTGCGCAAAAGGAATGGAGTAAGTTAGCTCCGACAATTCAGAGTCAGTTCAA
GAAGAAGTTAAAGGAACGATTAGAAAATCCGCACGTCCCTTCGGCTAAGCTTCGAGGATACGACGCTGTTTACAAGATTA
AACTTCGTACGGCGGGTTATCGTTTAGCTTATGAGGTTATTGACGATGAGATAGTTGTCTATGTCCTTGCTGTCGGTAAA
CGTGACAAAGATGCTGTCTATAAAAAACTGGCTTCACGCTTCGGTTAG

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9136.29 Da        Isoelectric Point: 4.0197

>AT30646 WP_001250179.1 NZ_AP014525:430821-431072 [Vibrio cholerae MS6]
MRQVLANCSASISELKKNPTALLNEADGSAIAILNHNKPAAYLVPAETYEYLIDMLDDYELSQIVDSRRADLAQAVEVNI
DDL

Download         Length: 252 bp

>AT30646 NZ_AP014525:430821-431072 [Vibrio cholerae MS6]
ATGAGACAAGTTTTAGCGAATTGCTCTGCAAGTATTTCAGAGTTAAAGAAGAACCCAACAGCTTTGTTGAATGAGGCTGA
TGGGTCTGCAATTGCAATTTTAAACCACAACAAGCCAGCGGCTTACCTTGTGCCAGCGGAAACGTATGAGTATCTCATCG
ATATGCTCGATGATTATGAGCTTTCCCAAATTGTCGATAGTCGCCGAGCTGACTTAGCACAAGCTGTGGAAGTAAATATT
GATGACCTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0X1KZS6

Antitoxin

Source ID Structure
AlphaFold DB A0A0X1KZS8

References